Development of a fluorescent immnunochromatographic assay for the procalcitonin detection of clinical patients in China

Wang, Haiying; Wei, Hong; Chen, Shaopei; Dzakah, Emmanuel Enoch; Kang, Keren; Wang, Jihua; Wang, Jufang

doi:10.1016/j.cca.2015.01.024

Cited by 15 publications

(7 citation statements)

References 25 publications

(26 reference statements)

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“…Fluorescent microspheres (FMs), which have a stable configuration and a high fluorescence intensity, are multicolored and safe. Due to the lack of sensitivity when applying AuNPs, FMs are used mainly for the detection of several compounds, such as aflatoxin M1 (AFM1) (98), tumor necrosis factor alpha (TNFα) [200], procalcitonin [201], 4(5)-methylimidazole [202], E.coli [203]. concentrations caused more interaction between E.coli and anti-E.coli antibody.…”

Section: Other Fluorescent Materialsmentioning

confidence: 99%

Lateral flow assays: Principles, designs and labels

Bahadır

Sezgintürk

2016

TrAC Trends in Analytical Chemistry

481

315

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Section: Other Fluorescent Materialsmentioning

confidence: 99%

Lateral flow assays: Principles, designs and labels

Bahadır

Sezgintürk

2016

TrAC Trends in Analytical Chemistry

481

315

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“…Several methods have been reported for PCT determination, mainly based in the selective recognition provided by immunological interactions. These methodologies include immunoturbidimetric assays [9], chemiluminescent immunoassays [10][11][12][13][14][15][16], immunochromatographic assays [17][18][19][20][21][22], surface plasmon resonance biosensors [23][24][25], fluorescence immunosensors [26][27][28][29][30][31][32], ellipsometry immunosensors [33], colorimetric immunoassays [34][35][36], and electrochemical immunoassays [37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56]. Even differen...…”

Section: Introductionmentioning

confidence: 99%

Magnetic Bead-Based Electrochemical Immunoassays On-Drop and On-Chip for Procalcitonin Determination: Disposable Tools for Clinical Sepsis Diagnosis

et al. 2020

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Procalcitonin (PCT) is a known protein biomarker clinically used for the early stages of sepsis diagnosis and therapy guidance. For its reliable determination, sandwich format magnetic bead-based immunoassays with two different electrochemical detection approaches are described: (i) disposable screen-printed carbon electrodes (SPE-C, on-drop detection); (ii) electro-kinetically driven microfluidic chips with integrated Au electrodes (EMC-Au, on-chip detection). Both approaches exhibited enough sensitivity (limit of detection (LOD) of 0.1 and 0.04 ng mL−1 for SPE-C and EMC-Au, respectively; cutoff 0.5 ng mL−1), an adequate working range for the clinically relevant concentrations (0.5–1000 and 0.1–20 ng mL−1 for SPE-C and EMC-Au, respectively), and good precision (RSD < 9%), using low sample volumes (25 µL) with total assay times less than 20 min. The suitability of both approaches was successfully demonstrated by the analysis of human serum and plasma samples, for which good recoveries were obtained (89–120%). Furthermore, the EMC-Au approach enabled the easy automation of the process, constituting a reliable alternative diagnostic tool for on-site/bed-site clinical analysis.

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“…Procalcitonin (PCT) is a small protein composed of 116 amino acids with a molecular weight of 13 kDa, 4 which is produced by C cells of the thyroid in healthy individuals. Normally, the PCT concentration in human serum from healthy individuals is very low (,0.01 ng/mL) and is undetectable via common methods.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a novel homogeneous nanoparticle-based assay for sensitive procalcitonin detection of ultra low-volume serum samples

Chen¹,

Liu²,

Li³

et al. 2018

IJN

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PurposeSepsis is a potentially fatal systemic body infection with a significant mortality rate worldwide. Although C-reactive protein (CRP), interleukin-6 (IL-6), and procalcitonin (PCT) might be biomarkers for sepsis diagnosis, PCT is more sensitive and specific than CRP or IL-6. We aimed to establish an efficient immunoassay that precisely detects PCT in human serum for the early diagnosis of sepsis.Materials and methodsWe developed a novel amplified luminescent proximity homogeneous assay (AlphaLISA) for the quantitative detection of PCT in serum. In this assay, a pair of antibodies was used to capture PCT in serum and to form sandwich complexes after incubating for 15 minutes at 37°C.ResultsPCT concentrations were determined within a linear range of 0.016–100 ng/mL. The limit of detection was 18.6 pg/mL. The results demonstrate that the reproducibility, recovery, and specificity of this assay for PCT meet the requirements of clinical detection. The coefficient of determination (R2) between this method and commercially available enzyme-linked fluorescent assay (ELFA) kits was estimated to be 0.93045 in clinical serum testing.ConclusionThe novel assay for PCT detection was robust with high sensitivity and a broad dynamic range. Compared with conventional heterogeneous detection methods such as ELISA, this assay measured the concentration of the homogeneous form of PCT and provided results that are more accurate within a shorter detection time. We expect that this novel method will be useful for the early screening and prognosis evaluation of patients with sepsis.

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Development of a fluorescent immnunochromatographic assay for the procalcitonin detection of clinical patients in China

Cited by 15 publications

References 25 publications

Lateral flow assays: Principles, designs and labels

Lateral flow assays: Principles, designs and labels

Magnetic Bead-Based Electrochemical Immunoassays On-Drop and On-Chip for Procalcitonin Determination: Disposable Tools for Clinical Sepsis Diagnosis

Establishment of a novel homogeneous nanoparticle-based assay for sensitive procalcitonin detection of ultra low-volume serum samples

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