A myocardial infarct border-zone-on-a-chip demonstrates distinct regulation of cardiac tissue function by an oxygen gradient

Rexius‐Hall, Megan L.; Khalil, Natalie; Escopete, Sean; Li, Xin; Hu, Jiayi; Yuan, Hongyan; Parker, Sarah J.; McCain, Megan L.

doi:10.1126/sciadv.abn7097

Cited by 6 publications

(9 citation statements)

References 84 publications

(96 reference statements)

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“…The constructed hypoxic microenvironment model is not accurate enough, thus affecting the experimental results. In vitro bone-on-a-chip platforms are expected to realize accurate reconstruction of the oxygen concentration and gradient observed in vivo [ 81 ].…”

Section: Construction Of Hypoxic Microenvironment Using Microfluidic ...mentioning

confidence: 99%

Construction of Bone Hypoxic Microenvironment Based on Bone-on-a-Chip Platforms

Zhao

Yang

et al. 2023

IJMS

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The normal physiological activities and functions of bone cells cannot be separated from the balance of the oxygenation level, and the physiological activities of bone cells are different under different oxygenation levels. At present, in vitro cell cultures are generally performed in a normoxic environment, and the partial pressure of oxygen of a conventional incubator is generally set at 141 mmHg (18.6%, close to the 20.1% oxygen in ambient air). This value is higher than the mean value of the oxygen partial pressure in human bone tissue. Additionally, the further away from the endosteal sinusoids, the lower the oxygen content. It follows that the construction of a hypoxic microenvironment is the key point of in vitro experimental investigation. However, current methods of cellular research cannot realize precise control of oxygenation levels at the microscale, and the development of microfluidic platforms can overcome the inherent limitations of these methods. In addition to discussing the characteristics of the hypoxic microenvironment in bone tissue, this review will discuss various methods of constructing oxygen gradients in vitro and measuring oxygen tension from the microscale based on microfluidic technology. This integration of advantages and disadvantages to perfect the experimental study will help us to study the physiological responses of cells under more physiological-relevant conditions and provide a new strategy for future research on various in vitro cell biomedicines.

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Section: Construction Of Hypoxic Microenvironment Using Microfluidic ...mentioning

confidence: 99%

Construction of Bone Hypoxic Microenvironment Based on Bone-on-a-Chip Platforms

Zhao

Yang

et al. 2023

IJMS

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“…130 To investigate the effects of different oxygen concentration gradients on cardiomyocytes, Rexius-Hall and his colleagues designed a myocardial infarct border-zone-on-a-chip. 131 They demonstrated that different oxygen gradients had an impact on calcium wave conduction velocity, systolic stress, and gene expression in cardiac tissue. These alterations resembled the characteristics of arrhythmia onset.…”

Section: Investigating the Development Of Cardiovascular Disease Usin...mentioning

confidence: 99%

Advances in organ‐on‐a‐chip for the treatment of cardiovascular diseases

Liu,

Wang

2023

MedComm – Biomaterials and Applications

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Cardiovascular disease (CVD) is currently a serious and growing public health problem. In tackling this challenge, organ‐on‐chip (OoC) technology, combined with cell culture and microfluidics, presents a powerful approach for constructing sophisticated tissue models in vitro that can simulate the physiological and pathological microenvironments of human organs. Nowadays, OoC technology has emerged as a pivotal tool in advancing our understanding of CVD pathogenesis, facilitating tissue regeneration studies, conducting efficient drug screening, and assessing therapeutic effects. Moreover, it offers a diverse array of study platforms for preclinical research, fostering innovative approaches towards combating CVD and improving patient outcomes. In this review, we first present the key advantages of OoC technology, including its highly relevant physiological microenvironment, incorporation of integrated functions, and the possibility of the construction of multiorgan‐on‐a‐chip through microfluidic linkage. Then, we summarized the role of OoC in the construction of disease pathological models, which provides a new channel for the exploration of disease pathological mechanisms. Moreover, we discuss the application of this technology in cardiac regeneration and drug screening. Finally, we discuss the challenges of tissue models constructed based on OoC technology and the prospects of this innovative approach.

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“…Reproduced under the terms of the Creative Commons Attribution‐NonCommercial license (CC BY‐NC). [ 126 ] Copyright 2022, The Authors, published by American Association for the Advancement of Science. c) The image of an assembled 15‐chamber HoC.…”

Section: Life Support System Inside Hoc Devicesmentioning

confidence: 99%

“…An easy yet effective approach to control oxygen concentration in cell culture chambers was reported by an oxygen permeable microfluidic device made out of PDMS (Figure 5b). [ 126 ] Cells were cultured under a 0–20% linear oxygen gradient via one channel continuously perfused with air and the other with nitrogen. Oxygen can also be consumed by pyrogallol, [ 127 ] dissolved ascorbate, [ 128 ] or Na 2 SO 3 [ 129 ] to recreate hypoxic (<10%) to anaerobic systems, or kept at atmospheric levels (21%), or increased to improve oxygenation of microtissues.…”

Section: Life Support System Inside Hoc Devicesmentioning

confidence: 99%

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Engineering Cardiac Tissue for Advanced Heart‐On‐A‐Chip Platforms

Chen,

Liu,

Han

et al. 2023

Adv Healthcare Materials

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Cardiovascular disease is a major cause of mortality worldwide, and current preclinical models including traditional animal models and 2D cell culture models have limitations in replicating human native heart physiology and response to drugs. Heart‐on‐a‐chip (HoC) technology offers a promising solution by combining the advantages of cardiac tissue engineering and microfluidics to create in vitro 3D cardiac models, which can mimic key aspects of human microphysiological systems and provide controllable microenvironments. Herein, recent advances in HoC technologies are introduced, including engineered cardiac microtissue construction in vitro, microfluidic chip fabrication, microenvironmental stimulation, and real‐time feedback systems. The development of cardiac tissue engineering methods is focused for 3D microtissue preparation, advanced strategies for HoC fabrication, and current applications of these platforms. Major challenges in HoC fabrication are discussed and the perspective on the potential for these platforms is provided to advance research and clinical applications.

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A myocardial infarct border-zone-on-a-chip demonstrates distinct regulation of cardiac tissue function by an oxygen gradient

Cited by 6 publications

References 84 publications

Construction of Bone Hypoxic Microenvironment Based on Bone-on-a-Chip Platforms

Construction of Bone Hypoxic Microenvironment Based on Bone-on-a-Chip Platforms

Advances in organ‐on‐a‐chip for the treatment of cardiovascular diseases

Engineering Cardiac Tissue for Advanced Heart‐On‐A‐Chip Platforms

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