A mutant strain of Chymomyza costata (Diptera: Drosophilidae) insensitive to diapause‐inducing action of photoperiod

Riihimaa, Ari; Kimura, Masahito T.

doi:10.1111/j.1365-3032.1988.tb01128.x

Cited by 68 publications

(48 citation statements)

References 3 publications

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“…Direct development was promoted under a constant temperature of 18°C and a long-day photoperiod (16 h light:8 h darkness), whereas diapause was induced by a constant temperature of 18°C and a short-day photoperiod (12 h light:12 h darkness). Under these conditions, all individuals responded reliably to the photoperiodic signal (24,26,55). Larvae were sampled at different phases of their nondiapause or diapause development based on our earlier observations (23,24,26).…”

Section: Methodsmentioning

confidence: 99%

Conceptual framework of the eco-physiological phases of insect diapause development justified by transcriptomic profiling

Košťál

Štětina

Poupardin

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

135

100

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Insects often overcome unfavorable seasons in a hormonally regulated state of diapause during which their activity ceases, development is arrested, metabolic rate is suppressed, and tolerance of environmental stress is bolstered. Diapausing insects pass through a stereotypic succession of eco-physiological phases termed “diapause development.” The phasing is varied in the literature, and the whole concept is sometimes criticized as being too artificial. Here we present the results of transcriptional profiling using custom microarrays representing 1,042 genes in the drosophilid fly, Chymomyza costata. Fully grown, third-instar larvae programmed for diapause by a photoperiodic (short-day) signal were assayed as they traversed the diapause developmental program. When analyzing the gradual dynamics in the transcriptomic profile, we could readily distinguish distinct diapause developmental phases associated with induction/initiation, maintenance, cold acclimation, and termination by cold or by photoperiodic signal. Accordingly, each phase is characterized by a specific pattern of gene expression, supporting the physiological relevance of the concept of diapause phasing. Further, we have dissected in greater detail the changes in transcript levels of elements of several signaling pathways considered critical for diapause regulation. The phase of diapause termination is associated with enhanced transcript levels in several positive elements stimulating direct development (the 20-hydroxyecdysone pathway: Ecr, Shd, Broad; the Wnt pathway: basket, c-jun) that are countered by up-regulation in some negative elements (the insulin-signaling pathway: Ilp8, PI3k, Akt; the target of rapamycin pathway: Tsc2 and 4EBP; the Wnt pathway: shaggy). We speculate such up-regulations may represent the early steps linked to termination of diapause programming.

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Section: Methodsmentioning

confidence: 99%

Conceptual framework of the eco-physiological phases of insect diapause development justified by transcriptomic profiling

Košťál

Štětina

Poupardin

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

135

100

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“…A non-photoperiodic diapause strain (npd) enters diapause when temperature decreases to 11°C, independently of photoperiod [169]. npd flies also exhibit circadian arrhythmicity in eclosion and oviposition, as well as in the circadian oscillation of per mRNA [170,171], suggesting that the circadian signalling pathway is disrupted in these flies.…”

Section: Circadian Signalling and Diapause: What Is The Link?mentioning

confidence: 99%

The hormonal and circadian basis for insect photoperiodic timing

2011

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Edited by Martha Merrow and Michael BrunnerKeywords: Insects Photoperiodism Diapause Endocrine axis Circadian clock Clock genes a b s t r a c t Daylength perception in temperate zones is a critical feature of insect life histories, and leads to developmental changes for resisting unfavourable seasons. The role of the neuroendocrine axis in the photoperiodic response of insects is discussed in relation to the key organs and molecules that are involved. We also discuss the controversial issue of the possible involvement of the circadian clock in photoperiodicity. Drosophila melanogaster has a shallow photoperiodic response that leads to reproductive arrest in adults, yet the unrivalled molecular genetic toolkit available for this model insect should allow the systematic molecular and neurobiological dissection of this complex phenotype.

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“…Such a striking difference in timeless expression between the two strains of C. costata appears interesting when related to npd-mutant's cir cadian and photoperiodic phenotypes. NPD strain flies are non-photoperiodic and behaviourally arrhythmic (Riihimaa & Kimura, 1988Kostal & Shimada, 2001) and it was confirmed by classical crossbreeding experi ments that both traits were caused by mutation in a single autosomal gene locus npd (Riihimaa & Kimura, 1989;Riihimaa, 1996;Lankinen & Riihimaa, 1992, 1997.…”

Section: Discussionmentioning

confidence: 96%

“…Two strains of Chymomyza costata were used for experiments: a wild-type (Sapporo) strain originally collected in Sapporo (43°N), Japan in 1983; a non-photoperiodic-diapause (npd)-mutant strain (NPD) which was isolated by Riihimaa & Kimura (1988) from wild-type flies collected in Tomakomai (42.3°N), Japan. Larvae, pupae and adults were cultured on an artificial diet of Lakovaara (1969) under a constant temperature of 18°C and a short-day (SD) photoperiod (10h light: 14h dark ness) that induces larval diapause in 100% of the population of Sapporo strain but no-diapause in the NPD strain.…”

Section: Insectsmentioning

confidence: 99%

“…The mature larvae of the wild-type (Sap poro) strain of the fly enter diapause in response to sub critically short days or low temperature (Riihimaa & Kimura, 1989;Kostal et al, 2000a). In 1983, Riihimaa & Kimura (1988) succeeded in selecting a mutant strain of flies which did not respond to a photoperiodic signal but were able to enter diapause at 11°C; the authors named the strain as NPD (Non-Photoperiodic-Diapause). Later, …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

TIMELESS: A link between fly's circadian and photoperiodic clocks?

Pavelka¹,

Shimada²,

Košťál³

2003

Eur. J. Entomol.

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Abstract. Potential involvement of circadian clock genes in so far unknown mechanism of photoperiodic time measurement is an important question of insect life-cycle regulation science. Here we report about the cloning of full-length cDNA of the structural homologue of the Drosophila's timeless gene in Chymomyza costata. Its expression was compared in two strains: a wild-type strain, responding to short days by entering larval diapause and a «pd-mutant strain, showing no photoperiodic response. The timeless mRNA transcripts were not detectable by Northern blot analysis in the fly heads of «pd-mutants, while they were detectable and showed typical daily oscillations in the wild-type strain. After disrupting the normal process of timeless transcription in the wild-type strain by injection of timeless double-strandRNA into early embryos of wild-type (RNAi method: Kennerdell & Carthew 1998, 2000, a certain proportion of the individuals adopted a «pd-mutant phenotype, showing no-diapause in response to short-daylength. Cloning of genomic DNA fragments revealed that «pd-mutants carry a different allele, timeless"1 "1, with a 13-bp insertion in an intron positioned within the 5'-leader sequence. Genetic linkage analysis showed that the 13-bp insertion (a marker for timeless"1 "1) and the absence of response to short days (a marker for «pd-phenotype) are strictly co-inherited in the F2 progeny of the reciprocal crosses between wild-type and «pd-mutant flies. Such results indicated that the locus «pd could code for the timeless gene in C. costata and its product might thus represent a molecular link between circadian and photoperiodic clock systems in this fly.

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A mutant strain of Chymomyza costata (Diptera: Drosophilidae) insensitive to diapause‐inducing action of photoperiod

Cited by 68 publications

References 3 publications

Conceptual framework of the eco-physiological phases of insect diapause development justified by transcriptomic profiling

Conceptual framework of the eco-physiological phases of insect diapause development justified by transcriptomic profiling

The hormonal and circadian basis for insect photoperiodic timing

TIMELESS: A link between fly's circadian and photoperiodic clocks?

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