A mutant ofDunaliella parva CCAP 19/9 leaking large amounts of glycerol into the medium

Hard, Barbara C.; Gilmour, D. James

doi:10.1007/bf00026100

Cited by 5 publications

(6 citation statements)

References 15 publications

(14 reference statements)

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“…A mutant strain of D. parva CCAP 19/9 was shown to leak large amounts of glycerol into the medium under isotonic conditions without a noticeable decrease in growth rate (Hard and Gilmour, ). This mutant arose spontaneously in a light‐limited chemostat culture, and the nature of the mutated gene is unknown.…”

Section: Dunaliella and Other Eukaryotic Microalgae As Producers Of Gmentioning

confidence: 99%

Glycerol metabolism in hypersaline environments

Oren

2016

Environmental Microbiology

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Summary Glycerol is a key compound for the understanding of the microbiology of hypersaline environments. At the highest salt concentrations the main or even sole primary producer is the green unicellular alga Dunaliella, which uses photosynthetically produced glycerol as osmotic stabilizer and compatible solute. Glycerol can be expected to be a major carbon source available to the heterotrophic communities of Archaea and Bacteria in hypersaline ecosystems. Use of Dunaliella has even been explored for the commercial production of glycerol. This article reviews our current understanding of glycerol metabolism in Dunaliella and of the ways glycerol can be degraded by heterotrophic prokaryote communities under aerobic and under anaerobic conditions. Dunaliella‐derived glycerol may also be the key toward long‐term survival of heterotrophic prokaryotes in fluid inclusions within salt crystals.

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Section: Dunaliella and Other Eukaryotic Microalgae As Producers Of Gmentioning

confidence: 99%

Glycerol metabolism in hypersaline environments

Oren

2016

Environmental Microbiology

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“…However, so far only a few mutants of the genus Dunaliella have been identified and employed (Brown et al, 1987;Hard and Gilmour, 1991;Shaish et al, 1991;Jin et al, 2001). In this work, we report the isolation and characterization of zea1, a zeaxanthin-accumulating mutant of the halotolerant green alga Dunaliella salina.…”

Section: Introductionmentioning

confidence: 99%

A mutant of the green alga Dunaliella salina constitutively accumulates zeaxanthin under all growth conditions

Jin

Feth

Melis

2002

Biotech & Bioengineering

109

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A novel mutant (zea1) of the halotolerant unicellular green alga Dunaliella salina is impaired in the zeaxanthin epoxidation reaction, thereby lacking a number of the beta-branch xanthophylls. HPLC analysis revealed that the zea1 mutant lacks neoxanthin (N), violaxanthin (V) and antheraxanthin (A) but constitutively accumulates zeaxanthin (Z). Under low-light physiological growth conditions, the zea1 (6 mg Z per g dry weight or 8 x 10(-16) mol Z/cell) had a substantially higher Z content than the wild type (0.2 mg Z per g dry weight or 0.5 x 10(-16) mol Z/cell). Lack of N, V, and A did not affect photosynthesis or growth of the zea1 strain. Biochemical analyses suggested that Z constitutively and quantitatively substitutes for N, V, and A in the zea1 strain. This mutant is discussed in terms of its commercial value and potential utilization by the algal biotechnology industry for the production of zeaxanthin, a high-value bioproduct.

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“…This paper describes our investigation into the uptake of glucose, acetate and glycerol by Dunaliella parva to examine what limits growth in the dark. In addition to the wild4ype strain, a mutant of D. parva which leaks large amounts of glycerol through the cell membrane into the medium (Hard & Gilmour, 1991) was also studied to test the hypothesis that the leaky mutant may take up organic compounds more readily than the wild-type and may therefore be a better candidate for heterotrophic growth.…”

Section: ) and Poterioochromonasmentioning

confidence: 99%

“…The mutant which leaks large amounts of glycerol arose spontaneously in a light-limited chemostat (Hard & Gilmour, 1991). Both strains were grown in 250 ml conical flasks with 150 ml of an artificial basal seawater medium containing either 0-4 or I'5 M NaCl buffered by 20 mM TRIS at pH 7"8 plus the following (in raM): Mg, 44; Ca, 10; K, 10; NO3, 5; PO 4, 0"1; SO 4, 48; FeEDTA, 1'5 × 10-3; HCO3, 1"2 × 10 2; H3BO 3, 0"185; Mn, 7 x 10 3; Zn 8 x 10-4; Co, 2 x 10-5; Cu, 2 x 10 7.…”

Section: Algal Culture and Growth Conditionsmentioning

confidence: 99%

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The uptake of organic compounds byDunaliella parvaCCAP 19/9

Hard

Gilmour

1996

European Journal of Phycology

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The feasibility of growing the unicellular green microalga DunaJielJa parva beterotrophically was investigated. D. parva cells were shown to take up [14C]glycerol and [~4C]glucose; the rate of uptake was fastest under illuminated conditions (photon flux density of 5 10 #tool m -2 s -1 ), but a significant rate of uptake was maintained in the dark for at least 6 h. The uptake of both compounds was stopped when photosynthesis and respiration were inhibited by adding carbonylcyanide m-chlorophenyl hydrazone (CCCP) or Gram's iodine to the cells. Subjecting the cells to increasing salinity, which reduces the rate of photosynthesis and respiration, also reduced the rate of [14C]glycerol uptake. These results suggested that the uptake of glycerol and glucose was due to active transport. Both transport systems were saturable and they were shown to be independent systems, since there was no effect on [14C]glycerol uptake when excess cold glucose was added or vice versa. D. parva cells could also take up [14C]acetate, but it was not determined whether this was by active transport. Despite the presence of active transport systems for glycerol and glucose and evidence of acetate uptake, D. parva was not able to grow heterotrophically on any of these three potential substrates.

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A mutant ofDunaliella parva CCAP 19/9 leaking large amounts of glycerol into the medium

Cited by 5 publications

References 15 publications

Glycerol metabolism in hypersaline environments

Glycerol metabolism in hypersaline environments

A mutant of the green alga Dunaliella salina constitutively accumulates zeaxanthin under all growth conditions

The uptake of organic compounds byDunaliella parvaCCAP 19/9

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