The uptake of organic compounds by<i>Dunaliella parva</i>CCAP 19/9

Hard, Barbara C.; Gilmour, D. James

doi:10.1080/09670269600651411

Cited by 14 publications

(3 citation statements)

References 22 publications

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“…( 1971 ) found that transport of glycerol by the bacteria, Pseudomonas aeruginosa, involves a binding protein responsible for recognition of glycerol. Additionally, studies with Dunaliella parva (Hard & Gilmour, 1996 ) showed that the uptake of glycerol is active, they confirmed the energy dependence of the glycerol transport system by its response to external salinity. These inconsistent results suggest that further investigations of glycerol transport in microalgae are warranted.…”

Section: Discussionmentioning

confidence: 80%

Osmotrophy of dissolved organic carbon by coccolithophores in darkness

2021

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Summary The evolutionary and ecological story of coccolithophores poses questions about their heterotrophy, surviving darkness after the end‐Cretaceous asteroid impact as well as survival in the deep ocean twilight zone. Uptake of dissolved organic carbon might be an alternative nutritional strategy for supply of energy and carbon molecules. Using long‐term batch culture experiments, we examined coccolithophore growth and maintenance on organic compounds in darkness. Radiolabelled experiments were performed to study the uptake kinetics. Pulse–chase experiments were used to examine the uptake into unassimilated, exchangeable pools vs assimilated, nonexchangeable pools. We found that coccolithophores were able to survive and maintain their metabolism for up to 30 d in darkness, accomplishing about one cell division. The concentration dependence for uptake was similar to the concentration dependence for growth in Cruciplacolithus neohelis, suggesting that it was taking up carbon compounds and immediately incorporating them into biomass. We recorded net incorporation of radioactivity into the particulate inorganic fraction. We conclude that osmotrophy provides nutritional flexibility and supports long‐term survival in light intensities well below threshold for photosynthesis. The incorporation of dissolved organic matter into particulate inorganic carbon, raises fundamental questions about the role of the alkalinity pump and the alkalinity balance in the sea.

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Section: Discussionmentioning

confidence: 80%

Osmotrophy of dissolved organic carbon by coccolithophores in darkness

2021

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“…as reported by Calixto et al (2016). While, it was reported that Dunaliella parva is able to grow heterotrophically utilizing glucose as an organic C-substrate for its growth (Hard & Gilmour, 1996).…”

Section: Resultsmentioning

confidence: 56%

Enhancement of Growth, Lipid, and Carbohydrate Production of the Egyptian isolate Dunaliella salina SA20 Using Mozzarella Cheese Whey as a Growth Supplement

Mohammad

Tawfeek²,

Eltanahy³

et al. 2022

Egypt. J. Bot.

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Bardawil Lake, Sinai, Egypt) in wastewater is a more cost-effective cultivation method.Wastewater, such as mozzarella cheese whey (MCW), is produced in massive quantities as a dairy industry byproduct, and growing D. salina in such waste could be used safely in biotechnological applications. Applying different organic MCW ratios to inorganic Loeblich nutrient medium (20, 50, 75% v/v MCW/Loeblich) as growth supplement allowed mixotrophic nutrition, while growth in completely inorganic medium (100% Loeblich medium) allowed for autotrophic nutrition. The optimum growth performance of D. salina cells was observed in 20% MCW and was indicated by measured values of chlorophyll a (10.51mg/L), dry weight (405mg/L), cell count (6.92 x 10 6 cell/ml), and optical density (1.12). Using 20% MCW, different pH values were applied to explore the pH effect on nutrient availability, and pH 7.5 was found to be optimal for growth. Additionally, a growth curve experiment comparing the mix-and autotrophic conditions presented higher values of the relative mean and maximum growth rates in mixotrophic conditions (0.19, 0.21 day −1 , respectively) than in the autotrophic conditions (0.16, 0.18 day −1 , respectively).

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“…Dunaliella salina CCAP 19/30 was supplied by the Culture Collection of Algae and Protozoa (CCAP), Oban, UK. The growth medium for Dunaliella, developed by Hard and Gilmour (1996), was used. Approximately 200 mL of freshly subcultured D. salina cells was added to the reactor until an initial optical density of 0.068 at 595 nm was achieved.…”

Section: Growth Of Dunaliella Salinamentioning

confidence: 99%

Enhancement of Dunaliella salina growth by using wavelength shifting dyes

2019

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The availability of light is one of the key driving factors for photosynthesis. Chlorophyll and accessory pigments absorb light at some of the wavelengths present in the solar spectrum. If all other requirements are available in abundance, introducing more light to the system at wavelengths that can be absorbed will increase the growth rate of microalgae and the final biomass achieved up to the level of photosaturation. Therefore, the effect of shifting the spectrum of light available such that the photons unutilized in photosynthesis are converted to those that can be utilised was investigated. Spectral shifts converting UV light to blue light and green light to red light were carried out using films produced with Coumarin and Solvent Orange respectively. Dunaliella salina (CCAP 19-30) was grown in an airlift photobioreactor (ALB) which was coated with the wavelength shifting films. Dunaliella final biomass in the ALB, as determined by the optical density, were shown to increase by 36.9% for UV to blue light conversion and by 18.8% for green to red light conversion when using a coated ALB compared with an uncoated one.

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The uptake of organic compounds byDunaliella parvaCCAP 19/9

Cited by 14 publications

References 22 publications

Osmotrophy of dissolved organic carbon by coccolithophores in darkness

Osmotrophy of dissolved organic carbon by coccolithophores in darkness

Enhancement of Growth, Lipid, and Carbohydrate Production of the Egyptian isolate Dunaliella salina SA20 Using Mozzarella Cheese Whey as a Growth Supplement

Enhancement of Dunaliella salina growth by using wavelength shifting dyes

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