A multiplex PCR-based DNA assay for the detection of paraoxonase gene cluster polymorphisms

Motti, Corradino; Dessı̀, Mariarita; Gnasso, Agostino; Irace, Concetta; Indigeno, Paola; Angelucci, Clotilde B.; Bernardini, Sergio; Fucci, Giorgio; Federici, Giorgio; Cortese, Claudio

doi:10.1016/s0021-9150(00)00765-6

Cited by 36 publications

(25 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Genomic DNA was extracted from peripheral blood lymphocytes, and primers for multiplex PCR amplification of PON1 55 and 192 were used according to Motti et al (2001).…”

Section: Methodsmentioning

confidence: 99%

PON1 55 and 192 Gene Polymorphisms in Type 2 Diabetes Mellitus Patients in a Turkish Population

et al. 2010

View full text Add to dashboard Cite

Diabetes mellitus is a multifactorial metabolic disease, caused by the complete or relative absence of insulin hormone, which results in the deterioration of carbohydrate, protein, and lipid metabolism. The PON1 55 and 192 polymorphisms have been reported to be associated with type 2 diabetes and its complications. In this study, the involvement of the PON1 55 and 192 polymorphisms and paraoxonase enzyme activity in diabetic complications was assessed. The MM and QQ genotypes were the most frequent in complications of type 2 diabetes in both of the polymorphisms. PON enzyme activity was lower in the type 2 diabetes group with respect to the control group. Regarding both genotypes and enzyme activity, correlations were found between the PON1 55 and 192 genotypes and diabetic complications. This study thus helps to outline a genotype-phenotype relation for the PON1 gene in a Turkish population.

show abstract

“…Genomic DNA was extracted from peripheral blood lymphocytes, and primers for multiplex PCR amplification of PON1 55 and 192 were used according to Motti et al (2001).…”

Section: Methodsmentioning

confidence: 99%

PON1 55 and 192 Gene Polymorphisms in Type 2 Diabetes Mellitus Patients in a Turkish Population

et al. 2010

View full text Add to dashboard Cite

show abstract

“…The Q192R and L55M polymorphisms of the PON1 gene were simultaneously studied by the protocol described previously by Motti et al [14] with some modifications. In brief, a multiplex polymerase chain reaction (PCR) method using specific primers corresponding to the PON1 nucleotide sequence was carried out, and the PCR products were digested with HinfI and then electrophoresed on 4% agarose gels stained with ethidium bromide.…”

Section: Genotype Analysismentioning

confidence: 99%

Effect of cigarette smoking on paraoxonase 1 activity according to PON1 L55M and PON1 Q192R gene polymorphisms

Mouhamed

Ezzaher

Mechri

et al. 2011

Environ Health Prev Med

View full text Add to dashboard Cite

Objective This study aims to investigate the effect of cigarette smoking on paraoxonase 1 (PON1) activity according to PON1 L55M and PON1 Q192R gene polymorphisms. Materials and methods Our sample included 300 voluntary subjects: 138 nonsmokers and 162 current smokers aged 38.47 ± 21.91 and 35.55 ± 16.03 years, respectively. PON1 activity was determined by kinetic methods. L55M and Q192R gene polymorphisms of PON1 were determined by multiplex polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP). Results We found in smokers a significant decrease of PON1 activity before and after adjustment. We noted a significant association between smoking status and lower PON1 activity [odds ratio (OR) = 3.03, confidence interval 95% = 1.5-5.9, p = 0.001]. In smokers, there was significant association between PON1 activity and PON1 L55M polymorphisms (p = 0.01). Also, the 55MM genotype presented the lowest paraoxonase activity, while the 55LL genotype showed the highest one. After adjustment for confounding variables, smokers with PON1 L55M polymorphism had the highest risk for lower PON1 activity; however, PON1 Q192R genotype might protect smokers from decrease in PON1 activity. We found significant interaction between the effect of cigarette smoking and both PON1 L55M and PON1 Q192R polymorphisms on lower PON1 activity. Conclusions Cigarette smoking was significantly associated with decrease in PON1 activity. Moreover, PON1 L55M polymorphism predisposes smokers to decreased PON1 activity in contrast to PON1 Q192R genotype.

show abstract

“…DNA was extracted from peripheral blood cells using the Ultraclean Bloodspin kit (MoBio Laboratories, Carlsbad, CA, USA). PON-1 genotyping was carried out by a multiplex PCR assay (Motti et al, 2001). Amplification of 111 and 144 pb fragments was performed using a standard PCR for PON-1-Q192R and PON-1-L55M, respectively.…”

Section: Methodsmentioning

confidence: 99%

Effect of walnut-enriched meat on the relationship between VCAM, ICAM, and LTB4 levels and PON-1 activity in ApoA4 360 and PON-1 allele carriers at increased cardiovascular risk

et al. 2011

View full text Add to dashboard Cite

Background/objective: Cardiovascular risk depends largely on paraoxonase (PON-1) and apolipoprotein A4 (APOA4) gene polymorphisms. To compare the effects of consumption of walnut-enriched meat versus low-fat meat (LM) on selected soluble adhesion molecules and leukotrienes (LTB4). Subjects/Methods: In all 22 subjects at increased cardiovascular risk were taken. It is a non-blinded, cross-over, placebocontrolled study. Two 5-week experimental periods separated by 4-6 week wash-out interval. Participants consumed walnutenriched meat during one period and LM during the other. Diet characteristics, HDLc, Apo A1, paraoxonase, sVCAM-1, sICAM-1 and LTB4 were analysed. PON-1 55, PON-1 192 and APOA4 360 polymorphism effects were also assessed. Results: Individuals consuming walnut-enriched meat displayed higher paraoxonase activity (Po0.001), lower levels of sICAM and aVCAM (P ¼ 0.046, P ¼ 0.012, respectively) and leukotriene B4 (P ¼ 0.044), and lower paraoxonase-1/HDLc and paraoxonase-1/Apo A1 ratios (both, Po0.001) than those consuming LM. Paraoxonase levels correlated negatively with those of sICAM (r ¼ À0.471, Po0.01). Significant decreases (at least Po0.05) were observed in sICAM concentrations in PON-1 55LM þ MM, PON-1 QQ192 and APOA4-2 carriers while decreases in sVCAM in QR þ RR and APOA4-1 carriers were observed. Paraoxonase-1/HDLc and paraoxonase-1/Apo A1 ratios were significantly influenced by paraoxonase polymorphisms. Conclusions: Walnut-enriched meat appears as a functional meat as consumed in the framework of a mix diet lowered the concentration of some selected inflammatory chemoattractant biomarkers. This effect was largely influenced by PON-1 and Apo A4-360 polymorphisms.

show abstract

A multiplex PCR-based DNA assay for the detection of paraoxonase gene cluster polymorphisms

Cited by 36 publications

References 24 publications

PON1 55 and 192 Gene Polymorphisms in Type 2 Diabetes Mellitus Patients in a Turkish Population

PON1 55 and 192 Gene Polymorphisms in Type 2 Diabetes Mellitus Patients in a Turkish Population

Effect of cigarette smoking on paraoxonase 1 activity according to PON1 L55M and PON1 Q192R gene polymorphisms

Effect of walnut-enriched meat on the relationship between VCAM, ICAM, and LTB4 levels and PON-1 activity in ApoA4 360 and PON-1 allele carriers at increased cardiovascular risk

Contact Info

Product

Resources

About