A multiparametric assay to compare the cytotoxicity of endodontic sealers with primary human osteoblasts

Scelza, Míriam Zaccaro; Linhares, Adriana; Silva, Licínio Esmeraldo da; Granjeiro, José Mauro; Alves, Gutemberg Gomes

doi:10.1111/j.1365-2591.2011.01941.x

Cited by 80 publications

(103 citation statements)

References 25 publications

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“…Our results revealed that both materials, AH Plus and MTA Fillapex, exhibited strong cytotoxic effects in freshly mixed conditions. This results is in agreement with previous studies that also observed severe cytotoxic effect of AH Plus and MTA Fillapex immediately after mixing and in the first days after mixing (4,7,10). After one week and in the succeeding weeks, AH Plus became non-cytotoxic, but MTA Fillapex toxicity did not decrease over time.…”

Section: Resultssupporting

confidence: 83%

“…However, concerning its cytototixicity and biocompatibility, controversial results were disclosed (4)(5)(6)(7)(8). While some studies demonstrated suitable biocompatibility (5) and bioactivity (6), other studies demonstrated poor biocompatibility and cytotoxicity (4,7,8). The biocompatibility of endodontic sealers is an important factor in choosing the best material, because endodontic sealers are often placed in intimate contact with the periapical tissues for an extended period.…”

Section: Introductionmentioning

confidence: 99%

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Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA Fillapex

et al. 2016

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The aim of the present study was to verify the long-term cytotoxic effects of the MTA Fillapex and to compare them with AH Plus. Dissolution rate and pH were also evaluated. Human osteoblast cells were incubated with elutes of fresh specimens from AH Plus and MTA Fillapex, and with elutes of the same specimens for 4 successive weeks. Elute's pH was evaluated at each time point. A multiparametric cell viability assay was performed. For dissolution rate, ISO methodology was used. The results were analyzed by one-way analysis of variance, complemented with the Tukey post-test (p<0.05). No significant difference was found among the materials when fresh mixed (p>0.05). After 1 week, AH Plus became non-cytotoxic on all three evaluated parameters. Conversely, MTA Fillapex remained severely and mildly cytotoxic over the entire experimental period (p<0.05). The dissolution rate of AH Plus was significantly lower than MTA Fillapex at all time points (p>0.05). The pH of AH Plus was significantly lower than MTA Fillapex at the second and third week (p<0.05). In the other tested time points no statistical difference was observed.In conclusion, MTA Fillapex remained cytotoxic after 4 weeks and its cytotoxicity may be related to the high dissolution rate of this material.

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Section: Resultssupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA Fillapex

et al. 2016

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“…(Machado and Oliveira 2014). In this present study, cell viability was determined by crystal violet assay, which is a test based on the ability of DNA from viable cells to capture the pigment (gentian violet), and neutral red assay (lysosomal function) (De Deus et al 2009;Scelza et al 2012). Both the tests (assays of some cellular functions) showed cell viability.…”

Section: Discussionmentioning

confidence: 99%

Viability of Human Gingival Fibroblast (FGH) Treated with Ethanolic "Aroeira" Extract (Myracrodruon urundeuva Allemão)

Machado¹,

Sartori²,

Damante³

et al. 2016

Braz. arch. biol. technol.

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“…Osteoblasts (15), mouse fibroblasts (16) and human fibroblasts (1) are some of the cell types usually employed in such investigations. Human fibroblasts were selected based on their relevance to clinical conditions.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Cytotoxicity of White MTA, MTA Fillapex® and Portland Cement on Human Periodontal Ligament Fibroblasts

et al. 2013

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The aim of this study was to compare the in vitro cytotoxicity of white mineral trioxide aggregate (MTA), MTA Fillapex ® and Portland cement (PC) on human cultured periodontal ligament fibroblasts. Periodontal ligament fibroblast culture was established and the cells were used for cytotoxic tests after the fourth passage. Cell density was set at 1.25 x10 4 cells/well in 96-well plates. Endodontic material extracts were prepared by placing sealer/ cement specimens (5x3mm) in 1mL of culture medium for 72 h. The extracts were then serially two-fold diluted and inserted into the cell-seeded wells for 24, 48 and 72 h. MTT assay was employed for analysis of cell viability. Cell supernatants were tested for nitric oxide using the Griess reagent system. MTA presented cytotoxic effect in undiluted extracts at 24 and 72 h. MTA Fillapex ® presented the highest cytotoxic levels with important cell viability reduction for pure extracts and at ½ and ¼ dilutions. In this study, PC did not induce alterations in fibroblast viability. Nitric oxide was detected in extract-treated cell supernatants and also in the extracts only, suggesting presence of nitrite in the soluble content of the tested materials. In the present study, MTA Fillapex displayed the highest cytotoxic effect on periodontal ligament fibroblasts followed by white MTA and PC.

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A multiparametric assay to compare the cytotoxicity of endodontic sealers with primary human osteoblasts

Abstract: All materials had high cytotoxic levels for human primary cells, mostly on a time-dependent basis, as shown by three different cell viability tests.

Cited by 80 publications

References 25 publications

Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA Fillapex

Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA Fillapex

Viability of Human Gingival Fibroblast (FGH) Treated with Ethanolic "Aroeira" Extract (Myracrodruon urundeuva Allemão)

In Vitro Cytotoxicity of White MTA, MTA Fillapex® and Portland Cement on Human Periodontal Ligament Fibroblasts

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