A multichannel nanosensor for instantaneous readout of cancer drug mechanisms

Rana, Subinoy; Le, Ngoc D. B.; Mout, Rubul; Saha, Krishnendu; Tonga, Gülen Yesilbag; Bain, Robert; Miranda, Oscar R.; Rotello, Caren M.; Rotello, Vincent M.

doi:10.1038/nnano.2014.285

Cited by 138 publications

(113 citation statements)

References 35 publications

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“…Recently, Rotello et al further advanced this GFP-AuNP conjugate sensor to profile chemotherapeutic mechanisms. 148 This sensor is composed of a benzyl head-group-terminated AuNP core and three different fluorescent proteins (EBFP2, EGFP, tdTomato). The drug-induced physicochemical changes on cancer cell surfaces can be “snified out” by the restored three-channel fluorescence.…”

Section: Aunp-based Fluorescent Assaysmentioning

confidence: 99%

Gold Nanoparticles for In Vitro Diagnostics

et al. 2015

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Section: Aunp-based Fluorescent Assaysmentioning

confidence: 99%

Gold Nanoparticles for In Vitro Diagnostics

et al. 2015

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“…25 A more powerful use of arrays is to target complex matrices, where all the analytes present may not be known. These applications include analyzing methods of drug action, 26 atmospheric analysis to protect against toxic gasses, 27 or sensing of disease for diagnosis and follow up. 28 A selective array can operate in a hypothesis-less mode, where samples are discriminated based on their complete, selective interaction, rather than on the basis of any single component.…”

Section: Hypothesis-free Universal Sensors?mentioning

confidence: 99%

Selectivity and Specificity: Pros and Cons in Sensing

2016

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Sensing using specific and selective receptors provides two very different but complementary strategies. This Sensor Issues article will discuss the merits and challenges of specific sensors, and selective sensors based on synthetic arrays. We will examine where each has been successfully applied to a sensing challenge, and then look at how a combined approach could take elements of both to provide new sensor platforms.

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“…Very recently, Rotello and coworkers have developed a high-throughput multi-channel sensor that classifies the mechanism of chemotherapeutic drugs in minutes. 49 This sensor consists of a single AuNP complexed with three different fluorescent proteins (FPs) that is used to sense drug-induced physicochemical changes on cell surfaces. In the presence of cells, differential displacement of the fluorophores with concomitant fluorogenesis provide a ratiometric output that is measurable from a single readout (Figure 10).…”

Section: Selective Sensingmentioning

confidence: 99%