A multichannel electrophoresis microchip platform for rapid chiral selector screening

Abstract

This study presents a four-channel electrophoresis chip platform, featuring double-cross hydrostatic sample injection, for rapid chiral selector screening. This platform needs only five electrodes to drive microchip electrophoresis in four separate channels for screening four chiral selectors at a time. To demonstrate the performance of this screening platform, eight neutral CDs and their derivatives as chiral selectors were screened towards two FITC-labeled chiral compounds. The screening could be accomplished in less than 2 min. Dimethyl-beta-CD and hydroxypropyl-alpha-CD was demonstrated to be the appropriate selectors for FITC-norfenefrine and FITC-baclofen, respectively. The established platform is easy to operate and suitable for rapid screening process, which is expected to be a potential platform for high-throughput screening of chiral selectors.

Chiral separations by CE employing CDs

“…The development of microchip technology was another interesting possibility to use CDs as chiral selector for the resolution of some enantiomers 98–111. The use of this electromigration technique is quite interesting, offering several possibilities for improving the analytical separation.…”

Progress in microchip enantioseparations

“…For enantioselective separations this approach was at first demonstrated by Gao et al [22,23]. Single channel, two channel and four channel microfluidic chips (Figs.…”

Advances in enantioselective separations using electromigration capillary techniques

“…In 2007 and the first half of 2008, four studies were published on that topic (Table 8). The group of Lin 147 presented a glass microchip fabricated by standard photolitography and wet chemical etching with four separate channels, which was used for the fast screening of eight neutral CDs as chiral SOs for enantioseparation of FITC‐labeled norfenefrine and baclofen in less than 2 min. The chip was composed of four identical units, each having independent sample injection and separation subunit.…”

“… (A) Photograph of the four‐channel microchip, (B) schematic of the partial channel structure and (C) fluorescence images of the double‐cross sample injection. Reprinted from 147, with permission. …”