Chiral separations by CE employing CDs

Fanali, Salvatore

doi:10.1002/elps.200900056

Cited by 120 publications

(80 citation statements)

References 114 publications

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“…Several reviews regarding chiral CE have appeared recently [9][10][11][12][13][14][15]. Enantioseparation can be achieved by adding many kinds of chiral selectors such as copper amino acid complexes [16], antibiotics [17,18] [19,20].…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a chiral capillary electrophoresis method for assay and enantiomeric purity control of pramipexole

Deng

Hai

Vervoort

et al. 2011

J of Separation Science

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A rapid method for the enantioseparation of pramipexole and its R-enantiomer has been developed by capillary electrophoresis. The influence of chemical and instrumental parameters was investigated including the type and concentration of chiral selectors, buffer composition and pH, co-ions, applied voltage, capillary length and temperature. Optimal separation conditions were obtained using a 50 mM phosphate buffer (pH 2.8) containing 25 mM carboxymethyl-β-cyclodextrin on a fused-silica capillary. Online UV detection was performed at 262 nm. A voltage of 25 kV was applied, and the capillary temperature was kept at 25°C. Hydrodynamic injection was performed at 3.45 kPa for 5.0 s. The separation of enantiomers was achieved in <6.5 min. The method was further validated in terms of stability of solutions, selectivity, linearity (both pramipexole and R-enantiomer, R(2) >0.995), LOD and LOQ (0.91 and 2.94 μg/mL, respectively), repeatability (RSD<1.5%) and accuracy (pramipexole, 100.4%; R-enantiomer, 100.5%). The proposed method was then applied to two kinds of pramipexole dihydrochloride monohydrate commercially available tablets, immediate release tablets (1.50 and 0.125 mg) and sustained release tablets (0.52 mg), to quantify the main component in the tablets. The amount of distomer could be quantified in bulk sample materials.

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Section: Introductionmentioning

confidence: 99%

Development and validation of a chiral capillary electrophoresis method for assay and enantiomeric purity control of pramipexole

Deng

Hai

Vervoort

et al. 2011

J of Separation Science

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show abstract

“…Based on the number of publications, CDs are by far the most often applied chiral selectors in CE as summarized recently in several reviews [34][35][36][37][38] and book chapters [39,40]. This is due to advantages such as UV transparency as well as the fact that many derivatives are commercially available and that they can be used in aqueous and nonaqueous background electrolytes.…”

Section: Chiral Separations Mechanisms In Capillary Electrophoresismentioning

confidence: 99%

Chiral electromigration techniques in pharmaceutical and biomedical analysis

Scriba

2011

Bioanal Rev

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Capillary electromigration techniques are often considered ideal methods for enantioseparations due to their high separation selectivity and flexibility. Thus, numerous methods employing a chiral selector as pseudostationary phase in a background electrolyte have been developed and applied for the chiral analysis of drugs in bulk ware, pharmaceutical formulations and biological matrices. Furthermore, electromigration techniques have been combined with spectroscopic methods such as nuclear magnetic resonance in order to understand the complexation of analytes by chiral selectors. The present review focuses on recent developments and applications of chiral electromigration techniques in pharmaceutical and biomedical analysis including examples illustrating analyte-selector complex formation or mechanistic studies which have been published between January 2009 and July 2011. Selector-mediated chiral separations clearly dominate while no applications of capillary electrochromatography to pharmaceutical or biomedical analysis have been reported during this period of time.

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“…low signal-tonoise ratio and data-sampling rate. In CE, the cyclodextrins (CDs) and their derivatives are undoubtedly the favourites as chiral selectors [31][32][33]. In this work, highly sulphated b-CD (HS-b-CD) is the chiral selector of choice; however, it has its problems which will be discussed later.…”

Section: Introductionmentioning

confidence: 99%

Determination of trace amount of enantiomeric impurity in therapeutic nicotine derivative using capillary electrophoresis with new imaging technology detection

Dahab

Smith

2011

J of Separation Science

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One of the many attempts to stop the danger of tobacco smoking is the development of an anti-smoking vaccine using nicotine butyric acid (NBA) linked to a carrier protein to produce anti-nicotine antibodies. NBA is a chiral molecule and there is a need to obtain a high degree of enantiomeric purity. The aim of this work is to develop a novel method for the enantioseparation of NBA and the determination of trace amounts of enantiomeric impurity required by regulatory authorities. This was achieved successfully using high-performance capillary electrophoresis combined with label-free intrinsic imaging as new imaging technology. A 50 μm id fused-silica capillary was used with UV detection at λ(214 ) nm and label-free intrinsic imaging. The background electrolyte consisted of highly sulphated β-cyclodextrin 10% m/V as a chiral selector in 75 mM phosphoric acid-triethylamine at pH 7.0. Baseline separation and detection of 0.1% and possibly less of the unwanted impurity (R-enantiomer) were achieved. Also, the detection limits were calculated for both enantiomers. The use of label-free intrinsic imaging has improved the sensitivity, enabling us to detect trace amounts of enantiomeric impurities.

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Chiral separations by CE employing CDs

Cited by 120 publications

References 114 publications

Development and validation of a chiral capillary electrophoresis method for assay and enantiomeric purity control of pramipexole

Development and validation of a chiral capillary electrophoresis method for assay and enantiomeric purity control of pramipexole

Chiral electromigration techniques in pharmaceutical and biomedical analysis

Determination of trace amount of enantiomeric impurity in therapeutic nicotine derivative using capillary electrophoresis with new imaging technology detection

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