A multicenter study on the performance of a fully automated, walk‐away high‐throughput analyzer for pretransfusion testing in the US population

Roback, John D.; Barclay, Sheilagh; Moulds, Joann M.; Denomme, Gregory A.

doi:10.1111/trf.13053

Cited by 13 publications

(21 citation statements)

References 23 publications

(20 reference statements)

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“…As in Chang et al [8], Cid et al [13] and Garozzo et al [11], the most frequently identified antibodies were anti-D (24.1%), in direct relationship with antenatal prophylaxis against haemolytic disease of the newborn, and anti-E (16.7%). Anti-K represented only 7.4% in our study while a greater percentage was described in Roback et al [9] and Tayler et al [10]. The concordance rate between Bio-Rad and Grifols is 100%, between Bio-Rad and OCD 91%, between OCD and Grifols 87% and between all three methods 91%.…”

Section: Discussioncontrasting

confidence: 49%

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A comparison of three column agglutination tests for red blood cell alloantibody identification

2020

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Objective: Commercial kits of column tests for pre-transfusion testing have progressively replaced conventional tube tests in most laboratories. Aim of this study was to compare three commercial test cell panels for the identification of irregular red blood cell (RBC) alloantibodies. Overall, 44 samples with a positive indirect antiglobulin test (IAT) by routine testing were used for comparison of following panels: Ortho RESOLVE ® panelC (Ortho Clinical Diagnostics (OCD), Milan, Italy), ID-DiaPanel(-P) (Bio-Rad Laboratories, CA, USA) and Identisera Diana(P) (Grifols, Barcelona, Spain). Column agglutination techniques were used, with microtubes containing either microgel (Bio-Rad/Grifols) or glass bead microparticles (Ortho). Results: Alloantibody identification was possible in 38 samples, of which identical identification was shown in 33 samples by all methods. The remaining samples showed differences between certain methods, with the gel card system being superior to the glass card system for analyzing stored samples Considering that not all samples were evaluated in all three methods, the concordance rate reached 100% between Bio-Rad and Grifols, 90.5% between Bio-Rad and OCD, 86.5% between OCD and Grifols and 90.5% between all methods. Although differences in sensitivities were seen for specific antibodies, the three methods showed comparable performance for the identification of RBC alloantibodies.

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Section: Discussioncontrasting

confidence: 49%

“…However, only few studies have focused on differences in identification of those RBC alloantibodies. Chang et al [8], Roback et al [9] and Taylor et al [10] compared test cell panels form Bio-Rad and Grifols for RBC antibody identification. Garozzo et al [11] compared results with test cell panels from OCD with those from Immucor.…”

Section: Introductionmentioning

confidence: 99%

A comparison of three column agglutination tests for red blood cell alloantibody identification

2020

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“…Following this, 50 l 1% RBCs was added to microtubes and centrifuged for 9 min using a preset cycle for antibody identification. The results of reactions were generated automatically through artificial vision technology and evaluated using a scale of strength as follows: negative (-) or increasingly positive at 1ϩ, 2ϩ, 3ϩ, or 4ϩ (20,57). The experiment was performed under the supervision of trained and experienced medical technicians.…”

Section: Methodsmentioning

confidence: 99%

Autoantibody-Mediated Erythrophagocytosis Increases Tuberculosis Susceptibility in HIV Patients

Dai

Cai

Wang

et al. 2020

mBio

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Macrophage dysfunction is associated with increased tuberculosis (TB) susceptibility in patients with human immunodeficiency virus (HIV) infection. However, the mechanisms underlying how HIV infection impairs macrophage function are unclear. Here, we found that levels of autoantibodies against red blood cells (RBCs) were significantly elevated in patients with HIV as determined by direct antiglobulin test (DAT). DAT positivity was significantly associated with TB incidence in both univariate and multivariate analyses (odds ratio [OR] = 11.96 [confidence interval {CI}, 4.68 to 30.93] and 12.65 [3.33 to 52.75], respectively). Ex vivo analysis showed that autoantibodies against RBCs enhanced erythrophagocytosis and thus significantly impaired macrophage bactericidal function against intracellular Mycobacterium tuberculosis. Mechanistically, autoantibody-mediated erythrophagocytosis increased heme oxygenase-1 (HO-1) expression, which inhibited M. tuberculosis-induced autophagy in macrophages. Silencing ATG5, a key component for autophagy, completely abrogated the effect of erythrophagocytosis on macrophage bactericidal activity against M. tuberculosis. In conclusion, we have demonstrated that HIV infection increases autoantibody-mediated erythrophagocytosis. This process impairs macrophage bactericidal activity against M. tuberculosis by inhibiting HO-1-associated autophagy. These findings reveal a novel mechanism as to how HIV infection increases TB susceptibility. IMPORTANCE HIV infection significantly increases TB susceptibility due to CD4 T-cell loss and macrophage dysfunction. Although it is relatively clear that CD4 T-cell loss represents a direct effect of HIV infection, the mechanism underlying how HIV infection dampens macrophage function is unknown. Here, we show that HIV infection enhances autoantibody-mediated erythrophagocytosis, which dampens macrophage bactericidal activity against TB by inhibiting HO-1-associated autophagy. Our findings reveal a novel mechanism explaining how HIV infection increases susceptibility to TB. We propose that DAT could be a potential measure to identify HIV patients who are at high TB risk and who would be suitable for anti-TB chemotherapy preventive treatment.

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“…As the scalable evolution of the Erytra® blood typing analyser (Chang et al ., ; Roback et al ., ), the new Erytra Eflexis® (Grifols) is a fully automated, benchtop, compact analyser for pre‐transfusion compatibility testing using the 8‐column DG Gel® card technology (Taylor et al ., ).…”

mentioning

confidence: 99%

Multicentre evaluation of Erytra Eflexis®, a benchtop fully automated analyser with a compact design for routine use in blood transfusion laboratory

Elliott

Carrascosa

Souchet

et al. 2019

Transfusion Medicine

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Objectives: Evaluation of the compact benchtop Erytra Eflexis ® automated analyser was performed at three health centres representing a range of routine transfusion workload.Background: Automation instruments with the simplicity and flexibility adequate for small-to mid-sized blood transfusion services are an unmet need. Methods: Performance in pre-transfusion testing (2109 ABO/D, 382 Rh/K phenotype, 2001 antibody screening, 113 antibody identification, 151 DAT, 88 extended phenotype; 655 cross matching) in comparison to Erytra ® as reference device was assessed. Throughput [time to first result (TTFR), final turn-around time (TAT), processing rate] was calculated; usability and adaptability in laboratory practice under routine and with emergency samples were surveyed.Results: Agreement between systems was 99·8% (11/5499 test discrepancies, all due to weak/doubtful positive reactions). Erytra Eflexis produced six true positives (two Rh/D, two B positives, two screening), four false positives (three screening and one cross matching) and one false negative (screening). Processing of eight routine samples with the Erytra Eflexis for ABO/Rh(D) and screening took 34-38 min and 32-37 min, respectively, independent of the simultaneous processing of a STAT sample, whether or not the incubator for STAT was reserved. In this scenario, a STAT sample requested within 2 min after the routine load was processed in 14-26 min. Processing rate tended to stabilise and optimise in the larger workloads, particularly in ABO/Rh(D)/K cards (16·7, 18 and 19·5 results/h for 10, 15 and 24 specimens, respectively). Conclusion:Erytra Eflexis analyser was found to be reliable and suitable for pre-transfusion routine tests performed in a small-/medium-sized blood transfusion laboratory.

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A multicenter study on the performance of a fully automated, walk‐away high‐throughput analyzer for pretransfusion testing in the US population

Abstract: Grifols' Erytra analyzer showed reliable efficacy compared with equivalent FDA-licensed reagents and FDA-cleared instruments.

Cited by 13 publications

References 23 publications

A comparison of three column agglutination tests for red blood cell alloantibody identification

A comparison of three column agglutination tests for red blood cell alloantibody identification

Autoantibody-Mediated Erythrophagocytosis Increases Tuberculosis Susceptibility in HIV Patients

Multicentre evaluation of Erytra Eflexis®, a benchtop fully automated analyser with a compact design for routine use in blood transfusion laboratory

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