A multi-step method for preparation of porcine small intestinal submucosa (SIS)

Luo, Jingcong; Chen, Wei; Chen, Xiaohe; Qin, Tingwu; Huang, Yong‐Can; Xie, Huiqi; Li, Xiuqun; Qian, Zhiyong; Yang, Zhi‐Ming

doi:10.1016/j.biomaterials.2010.09.017

Cited by 130 publications

(117 citation statements)

References 48 publications

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“…Porcine SIS was manufactured following the protocol of Luo's et al (2011). MSCs were labeled with 10 mmol/L bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) (Solarbio, Beijing, China), and then seeded on 1.5 cm Â 1.8 cm wetted SIS.…”

Section: Construction Of Neovaginamentioning

confidence: 99%

Bone marrow mesenchymal stem cells could acquire the phenotypes of epithelial cells and accelerate vaginal reconstruction combined with small intestinal submucosa

Liu

Zhang

et al. 2015

Cell Biology International

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Grafting material for vaginal reconstruction commonly includes the bowel, peritoneum, skin, and amniotic membrane. Bone marrow mesenchymal stem cells (MSCs) have the potential of multilineage differentiation into a variety of cells and have been widely explored in tissue engineering. In the current study, we examined whether MSCs could be differentiated to vaginal epithelial cells (VECs) upon co-culturing with VECs. We also examined whether Wnt/β-catenin signaling pathway is implicated in such differentiation. Co-culture of MSCs with VECs using a transwell insert system (with no direct contact) induced the expression of VECs marker AE1/AE3 in MSCs. MSCs combined with small intestinal submucosa (SIS) scaffold were implanted in place of the native vagina in rats to observe the implications for vaginal reconstruction in vivo. Anatomic repair of neovagina was assessed by histological staining for H/E and Masson's Trichrome. GSK-3β and β-catenin, main members of Wnt/β-catenin signaling pathway, in MSCs were increased upon co-culturing with VECs. Exposure of co-cultured MSCs to a Wnt/β-catenin signaling activator, lithium chloride (LiCl, 20 µM) increased phosphorylated GSK-3β and β-catenin and enhanced expression of AE1/AE3. In vivo-grafted cells displayed significant matrix infiltration and expressed epithelial markers in neovagina. These findings suggest that MSCs could acquire the phenotype of VECs when co-cultured with VECs, possibly via activation of Wnt/β-catenin signaling. MSCs provide an alternative cell source for potential use in vaginal tissue engineering.

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Section: Construction Of Neovaginamentioning

confidence: 99%

Bone marrow mesenchymal stem cells could acquire the phenotypes of epithelial cells and accelerate vaginal reconstruction combined with small intestinal submucosa

Liu

Zhang

et al. 2015

Cell Biology International

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“…32 In brief, a segment of fresh porcine jejunum was obtained from a local slaughterhouse. After removing the intestinal contents, the small intestine was cut into lengths of *10 cm each.…”

Section: Preparation Of Sismentioning

confidence: 99%

Reconstruction of Abdominal Wall Musculofascial Defects with Small Intestinal Submucosa Scaffolds Seeded with Tenocytes in Rats

Song

Peng

Liu

et al. 2013

Tissue Engineering Part A

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The repair of abdominal wall defects following surgery remains a difficult challenge. Although multiple methods have been described to restore the integrity of the abdominal wall, there is no clear consensus on the ideal material for reconstruction. This study explored the feasibility of in vivo reconstruction of a rat model of an abdominal wall defect with a composite scaffold of tenocytes and porcine small intestinal submucosa (SIS). In the current study, we created a 2 · 1.5 cm abdominal wall defect in the anterolateral abdominal wall of SpragueDawley rats, which were assigned into three groups: the cell-SIS construct group, the cell-free SIS scaffold group, and the abdominal wall defect group. Tenocytes were obtained from the tendons of rat limbs. After isolation and expansion, cells (2 · 10 7 /mL) were seeded onto the three-layer SIS scaffolds and cultured in vitro for 5 days. Cell-SIS constructs or cell-free constructs were implanted to repair the abdominal wall defects. The results showed that the tenocytes could grow on the SIS scaffold and secreted corresponding matrices. In addition, both scaffolds could repair the abdominal wall defects with no hernia recurrence. In comparison to the cell-free SIS scaffold, the composite scaffold exhibited increased vascular regeneration and mechanical strength. Furthermore, following increased time in vivo, the mechanical strength of the composite scaffold became stronger. The results indicate that the composite scaffold can provide increased mechanical strength that may be suitable for repairing abdominal wall defects.

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“…In contrast, chemical techniques appear more efficient, mainly involving the use of detergents, such as Triton X-100, Triton X-200, sodium dodecyl sulfate (SDS) and 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) [36]. Then there are enzymatic methods of decellularization which are based on the use of nucleases, proteases and calcium chelating agents [37]. Additionally, antibiotics and protease inhibitors are used respectively to eliminate the presence of bacteria and protect the native layer of collagen while proteases are being applied.…”

Section: Small Intestinal Submucosa (Sis)mentioning

confidence: 99%

Are agricultural and natural sources of bio-products important for modern regenerative medicine? A review

Nowacki

Nowacka

Kloskowski

et al. 2017

Ann Agric Environ Med.

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Introduction and objectives. As tissue engineering and regenerative medicine have continued to evolve within the field of biomedicine, the fundamental importance of bio-products has become increasingly apparent. This true not only in cases where they are derived directly from the natural environment, but also when animals and plants are specially bred and cultivated for their production. Objective. The study aims to present and assess the global influence and importance of selected bio-products in current regenerative medicine via a broad review of the existing literature. In particular, attention is paid to the matrices, substances and grafts created from plants and animals which could potentially be used in experimental and clinical regeneration, or in reconstructive procedures. Summary. Evolving trends in agriculture are likely to play a key role in the future development of a number of systemic and local medical procedures within tissue engineering and regenerative medicine. This is in addition to the use of bio-products derived from the natural environment which are found to deliver positive results in the treatment of prospective patients.

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A multi-step method for preparation of porcine small intestinal submucosa (SIS)

Cited by 130 publications

References 48 publications

Bone marrow mesenchymal stem cells could acquire the phenotypes of epithelial cells and accelerate vaginal reconstruction combined with small intestinal submucosa

Bone marrow mesenchymal stem cells could acquire the phenotypes of epithelial cells and accelerate vaginal reconstruction combined with small intestinal submucosa

Reconstruction of Abdominal Wall Musculofascial Defects with Small Intestinal Submucosa Scaffolds Seeded with Tenocytes in Rats

Are agricultural and natural sources of bio-products important for modern regenerative medicine? A review

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