A multi‐repeat adhesin of the phytopathogen, <i>Pectobacterium atrosepticum</i>, is secreted by a Type I pathway and is subject to complex regulation involving a non‐canonical diguanylate cyclase

Pérez-Mendoza, Daniel; Coulthurst, Sarah J.; Humphris, Sonia; Campbell, Emma; Welch, Martin; Toth, Ian K.; Salmond, George P. C.

doi:10.1111/j.1365-2958.2011.07849.x

Cited by 59 publications

(51 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The LapD/LapG proteolysis regulatory system is conserved in a wide variety of bacteria, some of which are pathogenic (67,303). Interestingly, in the phytopathogen Pectobacterium atrosepticum, the expression of the LapA adhesin is regulated by a DGC and a PDE encoded downstream of the operon (115). These enzymes are not conserved in P. fluorescens, which suggests that they respond to different environmental signals.…”

Section: Regulation Of Biofilmsmentioning

confidence: 99%

“…4A and 5A; Table 4). However, subsequently, more relaxed residue conservation requirements were observed (115). It is possible that the RYGGEEF active site motif found in the PleD, WspR, and HmsT proteins does indeed require strict conservation of all residues surrounding the catalytic Glu81 residue (38,94).…”

Section: Proteins With Ggdef and Eal Or Hd-gyp Domains Arranged In Tamentioning

confidence: 99%

“…In contrast, an RxGGDEF motif with a catalytic Asp81 residue may accommodate several different hydrophobic residues in the "x" position. In addition, it apparently retains some DGC activity even when the first Gly is replaced with Ala or Ser (115).…”

Section: Proteins With Ggdef and Eal Or Hd-gyp Domains Arranged In Tamentioning

confidence: 99%

“…Ca 2ϩ distorts the distances within the cluster, which explains its strong inhibitory effect. The a Activity data are as described previously (38,94,115). The RxGGDEF motif appears to tolerate a large variety of residues in the second (x) position, whereas the work reported in reference 94 suggests that the GGEEF motif is active only in the RYGGEEF variant, which is found in ϳ1/3 of RxGGEEF contexts.…”

Section: Figmentioning

confidence: 99%

See 3 more Smart Citations

Cyclic di-GMP: the First 25 Years of a Universal Bacterial Second Messenger

Römling

Galperin

Gomelsky

2013

Microbiol Mol Biol Rev

1,437

1,638

View full text Add to dashboard Cite

SUMMARY Twenty-five years have passed since the discovery of cyclic dimeric (3′→5′) GMP (cyclic di-GMP or c-di-GMP). From the relative obscurity of an allosteric activator of a bacterial cellulose synthase, c-di-GMP has emerged as one of the most common and important bacterial second messengers. Cyclic di-GMP has been shown to regulate biofilm formation, motility, virulence, the cell cycle, differentiation, and other processes. Most c-di-GMP-dependent signaling pathways control the ability of bacteria to interact with abiotic surfaces or with other bacterial and eukaryotic cells. Cyclic di-GMP plays key roles in lifestyle changes of many bacteria, including transition from the motile to the sessile state, which aids in the establishment of multicellular biofilm communities, and from the virulent state in acute infections to the less virulent but more resilient state characteristic of chronic infectious diseases. From a practical standpoint, modulating c-di-GMP signaling pathways in bacteria could represent a new way of controlling formation and dispersal of biofilms in medical and industrial settings. Cyclic di-GMP participates in interkingdom signaling. It is recognized by mammalian immune systems as a uniquely bacterial molecule and therefore is considered a promising vaccine adjuvant. The purpose of this review is not to overview the whole body of data in the burgeoning field of c-di-GMP-dependent signaling. Instead, we provide a historic perspective on the development of the field, emphasize common trends, and illustrate them with the best available examples. We also identify unresolved questions and highlight new directions in c-di-GMP research that will give us a deeper understanding of this truly universal bacterial second messenger.

show abstract

Section: Regulation Of Biofilmsmentioning

confidence: 99%

Section: Proteins With Ggdef and Eal Or Hd-gyp Domains Arranged In Tamentioning

confidence: 99%

Section: Proteins With Ggdef and Eal Or Hd-gyp Domains Arranged In Tamentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

See 2 more Smart Citations

Cyclic di-GMP: the First 25 Years of a Universal Bacterial Second Messenger

Römling

Galperin

Gomelsky

2013

Microbiol Mol Biol Rev

1,437

1,638

View full text Add to dashboard Cite

show abstract

“…The resulting protein is not greatly modified in activity, but it is smaller, more basic, and the modified form acts as a defense elicitor in plants (144). Recently, Pérez-Mendoza et al (122) showed that a second T1SS, which is regulated by a diguanylate cyclase and secretes a multi-repeat adhesin, is also important for Pectobacterium virulence. Figure 1: Pectobacterium.…”

Section: Type I Secretion System: Metalloproteases and Adhesinsmentioning

confidence: 99%

The Role of Secretion Systems and Small Molecules in Soft-Rot Enterobacteriaceae Pathogenicity

Charkowski¹,

Blanco

Condemine

et al. 2012

Annu. Rev. Phytopathol.

208

235

View full text Add to dashboard Cite

Soft-rot Enterobacteriaceae (SRE), which belong to the genera Pectobacterium and Dickeya, consist mainly of broad host-range pathogens that cause wilt, rot, and blackleg diseases on a wide range of plants. They are found in plants, insects, soil, and water in agricultural regions worldwide. SRE encode all six known protein secretion systems present in gram-negative bacteria, and these systems are involved in attacking host plants and competing bacteria. They also produce and detect multiple types of small molecules to coordinate pathogenesis, modify the plant environment, attack competing microbes, and perhaps to attract insect vectors. This review integrates new information about the role protein secretion and detection and production of ions and small molecules play in soft-rot pathogenicity.

show abstract

Quantification of Cyclic Dinucleotides by Reversed-Phase LC-MS/MS

Burhenne

Kaever

2013

Cyclic Nucleotide Signaling in Plants

View full text Add to dashboard Cite

Cyclic dinucleotides such as bis-(3',5')-cyclic dimeric adenosine monophosphate (c-di-AMP) and bis-(3',5')-cyclic dimeric guanosine monophosphate (c-di-GMP) represent important second messengers in bacteria. Although their synthesis has not been described in plants so far, they may be involved in the regulation of bacterial phytopathogen-plant interactions as well as rhizobium plant symbiosis. Here, we describe a sensitive and specific quantification method for c-di-AMP and c-di-GMP by HPLC-coupled tandem mass spectrometry. Additional linear dinucleotide metabolites and mononucleotides, as well as cyclic mononucleotides, can be simultaneously determined by this method.

show abstract

A multi‐repeat adhesin of the phytopathogen, Pectobacterium atrosepticum, is secreted by a Type I pathway and is subject to complex regulation involving a non‐canonical diguanylate cyclase

Cited by 59 publications

References 63 publications

Cyclic di-GMP: the First 25 Years of a Universal Bacterial Second Messenger

Cyclic di-GMP: the First 25 Years of a Universal Bacterial Second Messenger

The Role of Secretion Systems and Small Molecules in Soft-Rot Enterobacteriaceae Pathogenicity

Quantification of Cyclic Dinucleotides by Reversed-Phase LC-MS/MS

Contact Info

Product

Resources

About