A multi-biosensor based on immobilized Photosystem II on screen-printed electrodes for the detection of herbicides in river water

Touloupakis, Eleftherios; Giannoudi, L.; Piletsky, Sergey A.; Guzzella, Licia; Pozzoni, F.; Giardi, Maria Teresa

doi:10.1016/j.bios.2004.08.035

Cited by 75 publications

(54 citation statements)

References 22 publications

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“…It is therefore necessary that effective herbicides are used to reduce unwanted competition. Linuron, prometryn, and metribuzin are inhibitors of photosynthesis and are generally used at pre-emergence or sometimes at post-emergence (Touloupakis et al 2005). When these herbicides are used at pre-emergence, they are absorbed through the plant shoots while they are still underground and kill or injure the shoots before they emerge from the soil.…”

Section: Introductionmentioning

confidence: 99%

Weed control in lentil (Lens culinaris) in eastern Turkey

Elkoca

Kantar

Zengin

2005

New Zealand Journal of Crop and Horticultural Science

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This study investigated the effect of 12 herbicide applications and hand weeding (once, twice, and repeated) in comparison with a weedy control on seed and total crop biomass yield of lentil (Lens culinaris) in 2000 and 2001 under the dry conditions of Erzurum (29°55'N and 41° 16'E at an altitude of 1850 m), Turkey. In both growing seasons, data were collected on the density and dry weight of weed species, density of lentil, seed yield, total crop biomass yield, and harvest index. Weed control applications significantly decreased density and dry weight of weeds and increased total crop biomass and seed yields by 49.2% (linuron plus prometryn) and 74.9% (prometryn plus fluazifop-pbutyl), compared with the unweeded control. Preemergence plus post-emergence combinations, however, gave no clear advantages over single applications of linuron, prometryn, and metribuzin. Metribuzin reduced plant density in wetter conditions of the second year. In conclusion, linuron and prometryn alone were effective in controlling weeds on lentils without apparent toxic effects. Hand weeding once was equally effective.

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Section: Introductionmentioning

confidence: 99%

Weed control in lentil (Lens culinaris) in eastern Turkey

Elkoca

Kantar

Zengin

2005

New Zealand Journal of Crop and Horticultural Science

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“…The deposited film is clearly seen and molecularly smooth. In the SEM observation, the deposited film was found to be composed of the thylakoid membrane/PEI bilayers and the thickness of (thylakoid membrane/PEI) 10 . Thus, the QCM frequency change of 35000 was converted to a thickness value of 630 nm with the above equation.…”

Section: Surface Characterization Of Immobilized Thylakoidmentioning

confidence: 98%

“…Membrane Films Figure 2 shows a SEM of (thylakoid membrane/PEI) 10 film deposited onto a gold-coated resonator electrode. The deposited film is clearly seen and molecularly smooth.…”

Section: Surface Characterization Of Immobilized Thylakoidmentioning

confidence: 99%

“…For example, biosensors for the detection of herbicides via phytotoxicity were constructed using isolated photosynthetic reaction center protein or thylakoid membrane from chloroplast as biosensing elements [7,8]. A photosystem II-based multibiosensor offered a sensitive detection method for herbicides and prescreening analyses of natural waters [9,10].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Photoactivity Characteristics of a Biodevice Using Primary Photosynthetic Reaction Centers

et al. 2005

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Films with alternating layers of thylakoid membrane from the cyanobacterium Spirulina platensis and the positively charged polyion poly(ethylenimine) (PEI) were prepared by means of alternate electrostatic layer-by-layer assembly. The thylakoid membrane/PEI bilayers were functionally and structurally characterized by visible spectra, electrochemical methods and SEM techniques, respectively. The films deposited onto gold electrodes were molecularly smooth and consistent with thylakoid membrane/PEI bilayers as observed by SEM. The photoactivity of the thylakoid membrane/PEI assembly was dependent on thickness of the film that increased with the number of bilayers. There was an obvious difference in the current responses of 1-and 5-bilayer thylakoid membrane/PEI films under illumination. The photosynthetic electron transfer process in the thylakoid membrane/PEI single-bilayer film was demonstrated by an inhibition of the photoactivity by herbicide. Biodevices for the detection of phytotoxicity were constructed using the isolated thylakoid membrane from chloroplast as biosensing elements by means of electrostatic layer-by-layer assembly.

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“…For this reason, the use of some triazine pesticides has been banned in some countries or their permitted levels in drinking water is very low, so that analytical procedures for quantitative determination of several triazines, as well as their degradation products, at low levels are often requested. In this sense, several analytical techniques have been developed, like HPLC (Katsumata et al, 2006), CG-MS, capillary electrophoresis (Frías et al, 2004), solid-phase micro-extraction coupling with GC, LC, ion mobility spectrometry (Garcia Galan et al, 2010;Mohammadi et al, 2009;Sanchez Ortega et al, 2009;Quintana et al, 2001) and with HPLC (Zhou et al, 230 2009;See et al, 2010), immunosensors (Bahnd et al, 2005) and multi-biosensor based on immobilized Photosystem II (Touloupakisa et al, 2005;Dong et al, 2009), micellar electrokinetic chromatography (Zhang et al, 2008), tandem techniques (Beale et al, 2009;Tsang et al,2009;Lacina et al, 2010) cyclic voltammetry (Fuchiwaki et al, 2009;Zapardiel et al, 2000) and differential-pulse polarography (Ignjatovic et al, 1993;Kubo et al, 2008;Vaz et al, 1996) on solid electrodes, photosynthetic electron transport (PET) electrochemical biosensors (Campàs, et al, 2008;Preuss & Hall, 1995), PET colorimetric detection (Brewster & Lightfield, 1993;Shao et al, 2002) and adsorptive stripping voltammetry in dispersed media (Pedredo et al, 1995). In the last years, the environmental pollution by pesticides has become in a serious problem especially in aquatic ecosystems, due to their heavy use in agriculture and to their persistence.…”

Section: Introductionmentioning

confidence: 99%