A Mule Cloned from Fetal Cells by Nuclear Transfer

Woods, G.L.; White, Kenneth L.; Vanderwall, Dirk K.; Li, Guang Peng; Aston, Kenneth I.; Bunch, Thomas D.; Meerdo, Lora N.; Pate, Barry J.

doi:10.1126/science.1086743

Cited by 287 publications

(149 citation statements)

References 4 publications

(3 reference statements)

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“…Mule fibroblasts from a 45-day-old mule fetus were obtained as described elsewhere (Woods et al 2003). Equine blood samples were obtained during routine diagnostic measures and were used for the generation of metaphase spreads as well as the isolation of unstimulated lymphocytes.…”

Section: Equine Metaphase Spreads and Cell Nucleimentioning

confidence: 99%

Parental genomes mix in mule and human cell nuclei

et al. 2009

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Whether chromosome sets inherited from father and mother occupy separate spaces in the cell nucleus is a question first asked over 110 years ago. Recently, the nuclear organization of the genome has come increasingly into focus as an important level of epigenetic regulation. In this context, it is indispensable to know whether or not parental genomes are spatially separated. Genome separation had been demonstrated for plant hybrids and for the early mammalian embryo. Conclusive studies for somatic mammalian cell nuclei are lacking because homologous chromosomes from the two parents cannot be distinguished within a species. We circumvented this problem by investigating the three-dimensional distribution of chromosomes in mule lymphocytes and fibroblasts. Genomic DNA of horse and donkey was used as probes in fluorescence in situ hybridization under conditions where only tandem repetitive sequences were detected. We thus could determine the distribution of maternal and paternal chromosome sets in structurally preserved interphase nuclei for the first time. In addition, we investigated the distribution of several pairs of chromosomes in human bilobed granulocytes. Qualitative and quantitative image evaluation did not reveal any evidence for the separation of parental genomes. On the contrary, we observed mixing of maternal and paternal chromosome sets.

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Section: Equine Metaphase Spreads and Cell Nucleimentioning

confidence: 99%

Parental genomes mix in mule and human cell nuclei

et al. 2009

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“…Somatic cell nuclear transfer (SCNT) has been successfully utilized in generating cloned animals in various mammalian species, such as sheep, cattle, mouse, goat, pig, cat, rabbit, rat, mule, and horse (Campbell et al 1996, Wilmut et al 1997, Cibelli et al 1998, Kato et al 1998, Wakayama et al 1998, Baguisi et al 1999, Polejaeva et al 2000, Chesne et al 2002, Shin et al 2002, Galli et al 2003, Woods et al 2003, Zhou et al 2003. At present, the SCNT technique involves the adoptive transfer of a donor somatic nucleus to another individual's enucleated oocyte, termed here as 'allogeneic SCNT'.…”

Section: Introductionmentioning

confidence: 99%

Improved efficiency of bovine cloning by autologous somatic cell nuclear transfer

Yang¹,

Li²,

Ma³

et al. 2006

Reproduction

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Somatic cell nuclear transfer (SCNT) has been used for the cloning of various mammals. However, the rates of successful, healthy birth are generally poor. To improve cloning efficiency, we report the utilization of an 'autologous SCNT' cloning technique in which the somatic nucleus of a female bovine donor is transferred to its own enucleated oocyte recovered by ovum pick up, in contrast to the routine 'allogeneic SCNT' procedure using oocytes from unrelated females. Our results showed that embryos derived from autologous SCNT have significantly higher developmental competence than those derived from allogeneic SCNT, especially at the eight-cell (60 vs 44%), morula (45 vs 36%), and blastocyst (38 vs 23%) stages. The pregnancy and birth rates were also higher for the autologous (39 and 23%), compared to the allogeneic (22 and 6%) SCNT groups. Genome-wide histone3-lysine9 methylation profiles reveal that autologous SCNT embryos have less epigenetic defects than the allogeneic SCNT embryos. This study indicates that autologous SCNT can improve the efficiency of bovine cloning with less reprogramming deficiency.

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“…Sheep , mice (Wakayama et al 1998), cows (Cibelli et al 1998, Kato et al 1998, Wells et al 1999, goat (Baguisi et al 1999), rabbit (Chesne et al 2002), pig (Polejaeva et al 2000), cat (Shin et al 2002), mule (Woods et al 2003), rat (Zhou et al 2003) and horse (Galli et al 2003) have been cloned from several adult cell types, including mammary gland , cumulus (Kato et al 1998, Wakayama et al 1998, Wells et al 1999, Chesne et al 2002, Shin et al 2002, epithelial (Kato et al 1998) andB cells (Hochedlinger &Jaenisch 2002). Sheep , cows (Cibelli et al 1998, Zakhartchenko et al 1999, goats (Baguisi et al 1999), mule (Woods et al 2003), rat (Zhou et al 2003) and horse (Galli et al 2003) have been cloned from fibroblasts.…”

Section: Introductionmentioning

confidence: 99%

Apoptosis in rabbit embryos produced by fertilization or nuclear transfer with fibroblasts and cumulus cells

Liu

Yao²,

Jiang³

et al. 2005

Reproduction

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In this study, we investigated the development, the cell number of the blastocyst, and apoptosis in rabbit nuclear transfer (NT) embryos derived from adult fibroblasts and cumulus cells as compared with embryos derived from in vivo fertilization and in vitro culture. The developmental rate and the total cell number of the blastocyst were significantly lower in NT embryos than in fertilized embryos (FEs). The type of donor cells did not affect the embryonic developmental rate and the total cell number of blastocysts in NT groups. The present study investigated the onset and the frequency of apoptosis in NT embryos and FEs by using a terminal deoxynucleotidyl transferase-mediated dUTP nick and labeling (TUNEL) assay. The earliest positive TUNEL signals were detected at the eight-cell stage in NT embryos and at the morula stage in FEs. The apoptotic index of the total blastocysts, the inner cell mass and the trophoderm was greatly higher in the NT embryos than in FEs. Moreover, the apoptotic index of the blastocyst from fibroblasts was significantly higher than that of the blastocyst from cumulus cells.

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A Mule Cloned from Fetal Cells by Nuclear Transfer

Cited by 287 publications

References 4 publications

Parental genomes mix in mule and human cell nuclei

Parental genomes mix in mule and human cell nuclei

Improved efficiency of bovine cloning by autologous somatic cell nuclear transfer

Apoptosis in rabbit embryos produced by fertilization or nuclear transfer with fibroblasts and cumulus cells

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