Parental genomes mix in mule and human cell nuclei

Hepperger, Claudia; Mayer, Andreas; Merz, Julia; Vanderwall, Dirk K.; Dietzel, Steffen

doi:10.1007/s00412-008-0200-6

Cited by 8 publications

(5 citation statements)

References 54 publications

(59 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Contrary to what has been observed by Bártová and coworkers (2001), we found that in both resting and stimulated neutrophils the segregation of homologous chromosomes in the nuclear lobes was not significantly different from random expectations. These results are in agreement with those recently published on human granulocytes by Hepperger et al (2009).…”

Section: Nuclear Organizationsupporting

confidence: 95%

Nuclear architecture of resting and LPS-stimulated porcine neutrophils by 3D FISH

et al. 2009

View full text Add to dashboard Cite

Neutrophils are essential components of the innate immune system due to their ability to kill and phagocytose invading microbes. They possess a lobulated nucleus and are capable of extensive and complex changes in response to bacterial stimulation. The aim of our study was to investigate whether the 3D nuclear organization of porcine neutrophils was modified upon stimulation. The organization of centromeres, telomeres, and chromosome territories (chromosomes 2, 3, 7, 8, 12, 13, and 17) was studied on structurally preserved nuclei using 3D fluorescence in situ hybridization, confocal microscopy, and image analysis. By differential labeling of centromeres of acrocentric and metacentric/submetacentric chromosomes, we showed that centromeres associated to form chromocenters but did so preferentially between chromosomes with the same morphology. Upon activation, some of these chromocenters dispersed. Telomeres were also found to form clusters, but their number remained unchanged in lipopolysaccharide-stimulated neutrophils. The analysis of the position of chromocenters and telomere clusters showed a more internal location of the latter compared to the former. The analysis of chromosome territories revealed that homologs were distributed randomly among lobes whatever the cell's status. The volume of these territories was not proportional to chromosome length, and some chromosomes (chr 3, 12, 13, and 17) were more prone to decondensation when neutrophils were stimulated. Thus, our study demonstrated that activation of neutrophils resulted in several modifications of their nuclear architecture: a decrease in the number of non-acrocentric chromocenters and the decondensation of several chromosomes.

show abstract

Section: Nuclear Organizationsupporting

confidence: 95%

Nuclear architecture of resting and LPS-stimulated porcine neutrophils by 3D FISH

et al. 2009

View full text Add to dashboard Cite

show abstract

“…However, data available on the spatial organization of the genome concern principally the lymphocytes while little information is available for phagocytes, which also play a key role in this process. Some studies have analysed the 3D nuclear organization of chromosome territories in murine macrophages (Mayer et al 2005;Hepperger et al 2008) and in human (Hepperger et al 2009) and porcine (Yerle-Bouissou et al 2009) neutrophils. The relation between nuclear position and gene activity status has been investigated in human neutrophils (Bártová et al 2002), and chicken (Stadler et al 2004) and murine (Hepperger et al 2008) macrophages but mainly in the context of cell differentiation.…”

Section: Communicated By G Materamentioning

confidence: 98%

Transcriptomic and nuclear architecture of immune cells after LPS activation

et al. 2011

View full text Add to dashboard Cite

Changes in the nuclear positioning of specific genes, depending on their expression status, have been observed in a large diversity of physiological processes. However, gene position is poorly documented for immune cells which have been subjected to activation following bacterial infection. Using a pig model, we focused our study on monocyte-derived macrophages and neutrophils, as they are the first lines of defence against pathogens. We examined whether changes in gene expression due to LPS activation imply that genes have repositioned in the nuclear space. We first performed a transcriptomic analysis to identify the differentially expressed genes and then analysed the networks involved during lypopolysaccharide/interferon gamma activation in monocyte-derived macrophages. This allowed us to select four up-regulated (IL1β, IL8, CXCL10 and TNFα) and four down-regulated (VIM, LGALS3, TUBA3 and IGF2) genes. Their expression statuses were verified by quantitative real-time RT-PCR before studying their behaviour in the nuclear space during macrophage activation by means of 3D fluorescence in situ hybridization. No global correlation was found between gene activity and radial positioning. Only TNFα belonging to the highly transcribed MHC region on chromosome 7 became more peripherally localized in relation to the less decondensed state of its chromosome territory (CT) in activated macrophages. The analysis of gene positioning towards their CT revealed that IL8 increases significantly its tendency to be outside its CT during the activation process. In addition, the gene to CT edge distances increase for the three up-regulated genes (IL8, CXCL10 and TNFα) among the four analysed. Contrarily, the four down-regulated genes did not change their position. The analysis of gene behaviour towards their CT was extended to include neutrophils for three (TNFα, IL8 and IL1β) up- and two (IGF2 and TUBA3) down-regulated genes, and similar results were obtained. The analysis was completed by studying the four up-regulated genes in fibroblasts, not involved in immune response. Our data suggest that relocation in the nuclear space of genes that are differentially expressed in activated immune cells is gene and cell type specific but also closely linked to the entire up-regulation status of their chromosomal regions.

show abstract

“…Applying the same technique and similar 3-D analysis, Hepperger et al [48] failed to find symmetric distribution of FISH signals in bi-lobed nuclei of human granulocytes in a total of 3 specimens. Perhaps this failure was due to the small number of cases and the very low proportion of them with cells with bilateral symmetry, or these specimens were from cases with immunologic deficiency.…”

Section: Summary: Intranuclear Order Of Bilateral Symmetry Supports Cmentioning

confidence: 99%

Nuclear Segmentation, Condensation and Bilateral Symmetry in Polymorphonuclear Leukocytes Reflect Genomic Order and Favor Immunologic Function

Chaudhuri¹,

Walther²

2012

Acta Haematol

View full text Add to dashboard Cite

Segmentation, condensation and bilateral symmetry of the nuclei of polymorphonuclear leukocytes seem related to their function. Segmentation of the nuclei into two or more lobes and their condensation facilitate their passage (diapedesis) through the endothelial layer of blood vessels to the extravasal space and subsequent locomotion through the interstitial compartment of different tissues. Bilateral symmetry of these nuclei along with their association to the cytoskeletal fibers contribute to their efficiency in locomotion by alignment of the axis of nuclear symmetry to the axis of cellular polarity, which orients towards the direction of locomotion in response to cytokines and other stimuli. Observations of the cytogenetic facets of intranuclear order support these assumptions.

show abstract

Parental genomes mix in mule and human cell nuclei

Cited by 8 publications

References 54 publications

Nuclear architecture of resting and LPS-stimulated porcine neutrophils by 3D FISH

Nuclear architecture of resting and LPS-stimulated porcine neutrophils by 3D FISH

Transcriptomic and nuclear architecture of immune cells after LPS activation

Nuclear Segmentation, Condensation and Bilateral Symmetry in Polymorphonuclear Leukocytes Reflect Genomic Order and Favor Immunologic Function

Contact Info

Product

Resources

About