A morphological and chromosomal study of blastocysts developing from morphologically suboptimal human pre-embryos compared with control blastocysts

Hardarson, Thorir

doi:10.1093/humrep/deg092

Cited by 96 publications

(58 citation statements)

References 37 publications

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“…Sjoblom et al [17] illustrated that when they cultured embryos that were classified as unsuitable for freezing or transfer on day 3, they still obtained a number of good quality blastocysts. Hardarson et al [18] also reported that good quality blastocysts can develop from embryos classified as suboptimal. Furthermore, Guerif et al [19] revealed that morphological assessment at days 1 and 2 represents a fairly low prediction of embryo viability.…”

Section: Discussionmentioning

confidence: 99%

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The clinical outcomes of day 3 4-cell embryos after extended in vitro culture

Zhao

Lian

et al. 2014

J Assist Reprod Genet

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Objective To evaluate the development potential and clinical significance of day 3 4-cell embryos after extended in vitro culture. Methods This study was a retrospective cohort study for patients with infertility treatment between January 2011 and July 2013. Patients undergoing blastocyst culture in controlled ovarian hyperstimulation cycles using surplus embryos were analyzed in the study. A total of 764 women undergoing blastocyst culture with 1,522 surplus 4-cell embryos on day 3 were analyzed. An additional 2,391 patients with embryos undergoing blastocyst culture during the same period with embryos having more blastomeres were chosen as control. Results After extended culture, 253 embryos from 183 cycles in the study group which developed to blastocysts were frozen, and 118 embryos were warmed in subsequent frozen embryo transfer cycles. Implantation rates, clinical pregnancy rates (PRs) and ongoing PRs were 33.3 %, 38.4 % and 31.4 %, respectively, which were similar to those of the control group. The singleton birth weights of newborns using these blastocysts showed no significant difference to that seen in the control group. Conclusion Surplus 4-cell embryos on day 3 displayed lower blastulation rates. However, once a blastocyst is obtained, it has equivalent clinical outcomes. Embryos that are developmentally lagging on day 3 can be observed in extended culture to increase the cumulative chances of a successful pregnancy.

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Section: Discussionmentioning

confidence: 99%

“…Many studies have shown that the chromosomal status of embryo may affect the embryo cleavage rate and morphology [18,23,24]. Most of the researchers used fluorescence in situ hybridization (FISH) techniques and found a higher incidence of chromosomal abnormality of cleavage-stage embryos with poor morphology.…”

Section: Discussionmentioning

confidence: 99%

The clinical outcomes of day 3 4-cell embryos after extended in vitro culture

Zhao

Lian

et al. 2014

J Assist Reprod Genet

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“…Earlier studies have suggested a potential link between morphology and aneuploidy [7][8][9]. The quality of the blastocyst has been associated with the results of comprehensive chromosome screening of trophectoderm biopsies [10].…”

Section: Discussionmentioning

confidence: 99%

Chromosomal Status Assessed by CGH after a Day-3 Biopsy, in Embryos Classified by Standard Scoring Methodology at Pronuclear, Cleavage and Blastocyst Stage

Anagnostopoulou¹

2017

EMIJ

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Background: The standard scoring methodology, combined with blastocyst culture remains a valuable tool for embryo selection. Nevertheless, the embryo assessment based exclusively on morphological traits does not provide information about the chromosomal status of the embryo. Objective:The purpose of the present study has been to investigate the association between embryo morphology and chromosomal ploidy. Materials and methods:A total of 196 embryos resulting of 30 PGS cycles were biopsied on day 3 and tested for aneuploidies by comparative hybridization array. The embryos were classified into groups according to their morphology at zygote, cleavage and blastocyst stage.Results: Significantly statistical differences in embryo ploidy were observed when optimal day 3 morphology embryos were compared to medium morphology embryos (30.63% versus 17.28% respectively). A significantly higher proportion of euploid embryos were found in the group that achieved the blastocyst stage than in the arrested embryos group (42.57% versus 7.36%). Conclusion:The results provide evidence that embryo morphology and aneuploidy are linked both at day 3 and blastocyst stage. However the chromosomal status of the embryo may not be predicted relying exclusively on morphological assessment.

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“…This assumption is partially based on the fact that at 4-8 cell pre-embryo stage is just beginning to make use of its own genomic expression (10,11) and aneuploid or suboptimal pre-embryos might not be detected. In spite of this, differential morphology of human 1-cell pronuclear oocytes in terms of nuclei morphology and the cytoplasmic movements have been strongly correlated with implantation and development to the blastocyst stage (1,12).…”

Section: Introductionmentioning

confidence: 99%

The impact of pronuclear orientation to select chromosomally normal embryos

Borges

Rossi

Farah

et al. 2005

J Assist Reprod Genet

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Purpose : To find an effective method to select embryos from ICSI with better chromosomal status when preimplantation-genetic-diagnosis (PGD) is not applied. Methods : Several morpholological evaluations were done in same embryos. Embryos from ICSI were classified on day 1 according to pronuclear-nucleoli arrangement. On day 3, classification was done according to number, fragmentation, size and shape of cells. In some patients, embryos exhibiting good quality on day 3 (at least six regular blastomeres with cell fragmentation lower than 20%) were also submitted to PGD, irrespective to pronuclearnucleoli morphology. Results : Forty-two per cent of normally fertilized embryos showed pronuclear-nucleoli-goodmorphology; from those, 86% were also classified as good quality on day 3. Good-quality embryos submitted to PGD have shown lower chromosomal abnormality rates when also classified as pronuclear-nucleoli-good-morphology. Conclusions : Pronuclear-nucleoli morphology seems to be correlated with PGD results. This criterion may prove useful for pre-select embryos with normal chromosomal package when PGD is not applied.

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A morphological and chromosomal study of blastocysts developing from morphologically suboptimal human pre-embryos compared with control blastocysts

Abstract: The study suggests that even if the IVF laboratory is on average making the correct decision about the potential of a pre-embryo, surplus pre-embryos that might become chromosomally normal blastocysts are still being discarded.

Cited by 96 publications

References 37 publications

The clinical outcomes of day 3 4-cell embryos after extended in vitro culture

The clinical outcomes of day 3 4-cell embryos after extended in vitro culture

Chromosomal Status Assessed by CGH after a Day-3 Biopsy, in Embryos Classified by Standard Scoring Methodology at Pronuclear, Cleavage and Blastocyst Stage

The impact of pronuclear orientation to select chromosomally normal embryos

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