A More Sophisticated Method of Determining the Fungicidal Effect of Water‐Insoluble Preparations with a Cell Harvester, Using Miconazole as an Example./Eine verbesserte Methode zur Bestimmung der Fungizidie von wasserunlöslichen Präparaten mit Hilfe eines Zellerntegerätes am Beispiel von Miconazol

Abstract

A method is presented which, for the first time, enables preparations to be tested costeffectively and on a large scale to determine their fungicidal effect. A possible residual effect of the preparation on the fungi during the incubation period is prevented by completely washing out any traces of preparations that are nearly insoluble in water from the fungal suspension by means of a cell harvester and using polyethylene glycol (PEG) 400 as the solvent. With its automated procedure, the method enables antimycotics to be tested in 8 or 12 concentrations with reproducible results. Despite the capital investment that is necessary, the new method is much more cost-effective than the original methods because the personnel involved per test compound can be considerably reduced.

Chemical composition, antioxidant and antimicrobial activities of essential oil of Thymus algeriensis wild-growing in Libya

“…The micromycetes were maintained on malt agar and the cultures stored at 4°C and sub-cultured once a month [25]. In order to investigate the antifungal activity of the essential oil, a modified microdilution technique was used [22][23][24]. The fungal spores were washed from the surface of agar plates with sterile 0.85% saline containing 0.1% Tween 80 (v/v).…”

“…The following Gram-negative bacteria were used: [22][23][24]. The bacterial suspensions were adjusted with sterile saline to a concentration of 1.0x10…”

Phenolics and antifungal activities analysis in industrial crop Jerusalem artichoke (Helianthus tuberosus L.) leaves

“…The spore suspension was adjusted with sterile saline solution to a concentration of approximately 1.0 × 10 5 in a final volume of 100 L per well. Microdilution tests (Hänel and Raether, 1988;Daouk et al, 1995;Wanger et al, 1995;Espinel-Ingroff et al, 2002;Favre et al, 2003;Prats et al, 2007) were performed in 96-well plates in order to determine the antifungal activity of the samples against phytopathogenic fungi E. turcicum and G. zeae. The samples were diluted in distilled water to give serial twofold dilutions that were added to each medium, resulting in concentrations ranging from 0.49 to 1000 g mL −1 .…”

Antimicrobial and cytotoxic activities of Alnus rugosa L. aerial parts and identification of the bioactive components

“…The micromycetes were maintained on malt agar and the cultures were stored at 4 • C and sub-cultured once a month. The antifungal assay was carried out by modified microdilution technique (Hanel and Raether, 1988;Espinel-Ingroff, 2001). The fungal spores were washed from the surface of agar plates with sterile 0.85% saline containing 0.1% Tween 80 (v/v).…”