A monoclonal antibody to OspA inhibits association of Borrelia burgdorferi with human endothelial cells

Abstract

Previously, it has been shown that polyclonal antibodies to Borrelia burgdorferi and some monoclonal antibodies (MAbs) to borrelia major surface proteins caused inhibition of adherence of the bacteria to cultured human umbilical vein endothelial (HUVE) cells. In this study, fragment antigen binding (Fab) molecules generated from the immunoglobulin G fraction of rabbit anti-recombinant OspA serum were found to inhibit the adherence of B. burgdorferi to HUVE cells by 73%. Subsequently, MAbs were generated for use in determining whether or how B. burgdorfiei outer surface proteins (Osps) A and/or B are involved in mediating attachment to, and/or invasion of, HUVE cells by B. burgdorferi. Twenty-two MAbs were generated to borrelial proteins with apparent molecular masses (determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis) of 19, 31 (OspA), 34 (OspB), and 35 kDa. Fab molecules from one anti-OspA MAb, 9B3D, demonstrated an inhibitory effect on bacterial association with HUVE cells. None of the other MAbs, including the other anti-OspA MAbs, showed an inhibitory effect on cell association of greater than 5%. This effect of Fab on August 5, 2020 by guest http://iai.asm.org/ Downloaded from on August 5, 2020 by guest http://iai.asm.org/ Downloaded from on August 5, 2020 by guest http://iai.asm.org/ Downloaded from INFECT. IMMUN.on August 5, 2020 by guest

Relationship between infectivity and OspC expression in Lyme disease Borrelia

“…Hughes et al [3] reported that an OspA and OspB-less mutant lost infectivity against hamster. Furthermore, an anti-OspA mAb inhibited adhesion of B. burgdorferi to the endothelial cell surface [4] and OspD was abundantly expressed on the virulent low-passage strain B31 in comparison with avirulent high-passage strain [5]. These findings indicate that three Osp molecules, OspA, OspB and OspD, play important roles in the adhesion to and/or penetration of host cells and are required for the initiation of infection.…”

Plasmid modifications in a tick‐borne pathogen, Borrelia burgdorferi, cocultured with tick cells

“…burgdorferi, and is the species of origin of IDES (Kurtti etal., 1993). More spirochetes per cell (up to fifteen to twenty, in 24 h; Kurtti, unpublished) bind to a higher percentage of cells in these two tick lines (90'Aor more: Kurtti eta/., 1993) than has been reported for other tick (Kurtti et a/., 1988b(Kurtti et a/., , 1993 or mammalian cells (Comstock et a/., 1993). Adhesion of Lyme disease borreliae to RAE25 and IDE8 tick cells in vitro is thus unlikely to represent a non-specific event (Benach eta/., 1991).…”

Molecular analysis of a 66-kDa protein associated with the outer membrane of Lyme disease Borrelia

“…genitally polymorphic [2,3]. They play an important role in the establishment of the infection [4] and the triggering of the anti-borrelial immune response [51. However, it is unclear whether heterogeneity of these proteins allows efficient cross-protection.…”