A monoclonal antibody to DIII E protein allowing the differentiation of West Nile virus from other flaviviruses by a lateral flow assay

Rebollo, Belén; Pérez, Teresa; Camuñas, Ana; Pérez‐Ramírez, Elisa; Llorente, Francisco Rubio; Sánchez-Seco, María Paz; Jiménez‐Clavero, Miguel Ángel; Venteo, Ángel

doi:10.1016/j.jviromet.2018.06.016

Cited by 5 publications

(2 citation statements)

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“…Therefore, after the invasion of a pathogen microorganism, the antibodies produced can be used as biomarkers for LFIA technology to determine whether it is infected. For example, its use in the diagnosis of pathogenic microbial infections such as Coccidioides ( Azeem et al, 2022 ), Chlamydia trachomatis ( Gwyn et al, 2019 ), and West Nile virus ( Rebollo et al, 2018 ) has had many applications, so LFIA techniques based on SARS-CoV-2 antibodies for the diagnosis of COVID-19 have been widely followed.…”

Section: Lfia Technique For Sars-cov-2 Antibody Detectionmentioning

confidence: 99%

Rapid detection of SARS-CoV-2: The gradual boom of lateral flow immunoassay

Zhu

Zhou

et al. 2023

Front. Bioeng. Biotechnol.

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Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is still in an epidemic situation, which poses a serious threat to the safety of people and property. Rapid diagnosis and isolation of infected individuals are one of the important methods to control virus transmission. Existing lateral flow immunoassay techniques have the advantages of rapid, sensitive, and easy operation, and some new options have emerged with the continuous development of nanotechnology. Such as lateral flow immunoassay test strips based on colorimetric-fluorescent dual-mode and gold nanoparticles, Surface Enhanced Raman Scattering, etc., these technologies have played an important role in the rapid diagnosis of COVID-19. In this paper, we summarize the current research progress of lateral flow immunoassay in the field of Severe Acute Respiratory Syndrome Coronavirus 2 infection diagnosis, analyze the performance of Severe Acute Respiratory Syndrome Coronavirus 2 lateral flow immunoassay products, review the advantages and limitations of different detection methods and markers, and then explore the competitive CRISPR-based nucleic acid chromatography detection method. This method combines the advantages of gene editing and lateral flow immunoassay and can achieve rapid and highly sensitive lateral flow immunoassay detection of target nucleic acids, which is expected to be the most representative method for community and clinical point-of-care testing. We hope that researchers will be inspired by this review and strive to solve the problems in the design of highly sensitive targets, the selection of detection methods, and the enhancement of CRISPR technology, to truly achieve rapid, sensitive, convenient, and specific detection of novel coronaviruses, thus promoting the development of novel coronavirus diagnosis and contributing our modest contribution to the world’s fight against epidemics.

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Section: Lfia Technique For Sars-cov-2 Antibody Detectionmentioning

confidence: 99%

Rapid detection of SARS-CoV-2: The gradual boom of lateral flow immunoassay

Zhu

Zhou

et al. 2023

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…After 10 minutes, in the presence of WNV, a red line is formed at the TL, informing the test positivity. This test was evaluated and successfully detected different WNV strains with LOD of 105 TCID50/mL and showed no cross-reactivity with other flaviviruses [ 174 ].…”

Section: Viral Infections Determination Using Lfa Sensing Platformsmentioning

confidence: 99%