Lateral flow assays (LFA) as an alternative medical diagnosis method for detection of virus species: The intertwine of nanotechnology with sensing strategies

Sadeghi, Poorya; Sohrabi, Hessamaddin; Hejazi, Maryam; Jahanban-Esfahlan, Ali; Baradaran, Behzad; Tohidast, Maryam; Majidi, Mir Reza; Mokhtarzadeh, Ahad; Tavangar, Seyed Mohammad; Guárdia, Miguel de la

doi:10.1016/j.trac.2021.116460

Cited by 61 publications

(36 citation statements)

References 172 publications

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“…It depends on fluid migration or flow method (Ben Aissa et al., 2017; O'Farrell, 2009). LFAs (Sadeghi et al., 2021) have currently been applied, to some extent, in quantitative and qualitative screening in nonlaboratory environments, which have received enormous interest as a result of their low costs, short analysis periods, friendly user formats, low interferences, and easy operation by nonspecialized staff (Bahadır & Sezgintürk, 2016). This method has been developed based on biochemical interactions between antigen (Ag) and Ab or hybridization between DNA probe and target.…”

Section: Introductionmentioning

confidence: 99%

State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

Sohrabi

Majidi

Khaki

et al. 2022

Comp Rev Food Sci Food Safe

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Diverse chemicals and some physical phenomena recently introduced in nanotechnology have enabled scientists to develop useful devices in the field of food sciences. Concerning such developments, detecting foodborne pathogenic bacteria is now an important issue. These kinds of bacteria species have demonstrated severe health effects after consuming foods and high mortality related to acute cases. The most leading path of intoxication and infection has been through food matrices. Hence, quick recognition of foodborne bacteria agents at low concentrations has been required in current diagnostics. Lateral flow assays (LFAs) are one of the urgent and prevalently applied quick recognition methods that have been settled for recognizing diverse types of analytes. Thus, the present review has stressed on latest developments in LFAs‐based platforms to detect various foodborne pathogenic bacteria such as Salmonella, Listeria, Escherichia coli, Brucella, Shigella, Staphylococcus aureus, Clostridium botulinum, and Vibrio cholera. Proper prominence has been given on exactly how the labels, detection elements, or procedures have affected recent developments in the evaluation of diverse bacteria using LFAs. Additionally, the modifications in assays specificity and sensitivity consistent with applied food processing techniques have been discussed. Finally, a conclusion has been drawn for highlighting the main challenges confronted through this method and offered a view and insight of thoughts for its further development in the future.

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Section: Introductionmentioning

confidence: 99%

State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

Sohrabi

Majidi

Khaki

et al. 2022

Comp Rev Food Sci Food Safe

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“…In a previous study, ultimate sensitivity was demonstrated for the Au/Pt-based LFA in detecting influenza virus A using LNSM and considering antigen–antibody affinity and lot-to-lot differences [ 6 , 13 ]. LNSM should have the highest LFA sensitivity as it involves direct monitoring of conventional metal particles.…”

Section: Discussionmentioning

confidence: 99%

Highly Sensitive and Quantitative Diagnosis of SARS-CoV-2 Using a Gold/Platinum Particle-Based Lateral Flow Assay and a Desktop Scanning Electron Microscope

et al. 2022

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The gold standard test for identifying SARS-CoV-2, the causative agent of COVID-19, is polymerase chain reaction (PCR). Despite their limited sensitivity, SARS-CoV-2 antigen rapid diagnostic tests are vital tools in the fight against viral spread. Owing to its simplicity and low cost, the lateral flow assay (LFA) is the most extensively used point-of-care diagnostic test. Here, we report a newly designed LFA-NanoSuit method (LNSM) that works in conjunction with desktop scanning electron microscopy (SEM) to detect SARS-CoV-2. LNSM requires no standard SEM treatment, avoids cellulose and residual buffer deformation, and enables the capture of high-resolution images of antibody-labeled gold/platinum particles reacting with SARS-CoV-2 antigens. To assess its applicability, we compared clinical SARS-CoV-2 samples via visual detection of LFA, LSNM detection of LFA, and real-time reverse transcription-PCR (qRT-PCR). Compared to qRT-PCR, LNSM showed 86.7% sensitivity (26/30; 95% confidence interval (CI): 69.28–96.24%) and 93.3% specificity (14/15; 95% CI: 68.05–99.83%) for SARS-CoV-2. In samples with a relatively low SARS-CoV-2 RNA copy number (30 < Ct ≤ 40), the sensitivity of LNSM was greater (73.3%) than that of visual detection (0%). A simple, sensitive, and quantitative LNSM can be used to diagnose SARS-CoV-2.

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“…The LFA technique for the early diagnosis has great potential for preventing, monitoring, and controlling COVID-19, especially in the post-pandemic era. However, only a few comprehensive reviews have focused on LFA ( Habli et al, 2021 ; Hsiao et al, 2021 ; Hsieh et al, 2021 ; Jia et al, 2021 ; Sadeghi et al, 2021 ; Yadav et al, 2021 ; Zhou et al, 2021 ). In this work, LFA that could be used for large-scale screening of COVID-19 at home, school, and under various non-laboratory scenarios owing to its simplicity, convenience, rapidity, and cost-efficiency is discussed.…”

Section: Introductionmentioning

confidence: 99%

Recent Progress on Rapid Lateral Flow Assay-Based Early Diagnosis of COVID-19

Zhang

Chai

et al. 2022

Front. Bioeng. Biotechnol.

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The outbreak of the coronavirus disease 2019 (COVID-19) has resulted in enormous losses worldwide. Through effective control measures and vaccination, prevention and curbing have proven significantly effective; however, the disease has still not been eliminated. Therefore, it is necessary to develop a simple, convenient, and rapid detection strategy for controlling disease recurrence and transmission. Taking advantage of their low-cost and simple operation, point-of-care test (POCT) kits for COVID-19 based on the lateral flow assay (LFA) chemistry have become one of the most convenient and widely used screening tools for pathogens in hospitals and at home. In this review, we introduce essential features of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, compare existing detection methods, and focus on the principles, merits and limitations of the LFAs based on viral nucleic acids, antigens, and corresponding antibodies. A systematic comparison was realized through summarization and analyses, providing a comprehensive demonstration of the LFA technology and insights into preventing and curbing the COVID-19 pandemic.

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Lateral flow assays (LFA) as an alternative medical diagnosis method for detection of virus species: The intertwine of nanotechnology with sensing strategies

Cited by 61 publications

References 172 publications

State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

Highly Sensitive and Quantitative Diagnosis of SARS-CoV-2 Using a Gold/Platinum Particle-Based Lateral Flow Assay and a Desktop Scanning Electron Microscope

Recent Progress on Rapid Lateral Flow Assay-Based Early Diagnosis of COVID-19

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