A Monoclonal Anti-Neuroblastoma Antibody That Discriminates Between Human Nonhematopoietic and Hematopoietic Cell Types

Abstract

A murine IgG2a monoclonal antibody, termed 6-19, was characterized in terms of its ability to bind to human cell lines and tissues. The hybridoma was selected for antibody binding to multiple human neuroblastoma cultured cell lines but not to peripheral blood mononuclear cells. 6-19 binds to the cell surface of all cultured human nonhematopoietic tumor cell lines tested, to cultured human fibroblasts and endothelial cells, and to nonhematopoietic tumors of many types. It does not bind detectably to any hematopoietic cells, leukemia cells, or lymphomas. In the presence of complement, 6-19 is very cytotoxic to cultured human neuroblastoma cells but not to bone marrow granulocyte-macrophage colony-forming cells. The 6-19 monoclonal antibody may prove useful in the identification or destruction of tumor and stromal cells in bone marrow.

A comparative review of the immunobiology of murine neuroblastoma and human neuroblastoma

“…40 Such antibodies would have further diagnostic or therapeutic application if issues regarding receptor specificity and cross-reactivity with neural tissue and normal adrenal tissue were resolved.Mo-Hellstrom et al first used in vitro assays in the study of host antitumor immune response; and with noclonal antibodies of murine origin have been raised to a glycolipid antigen on human NB cells 41,42 but not the colony inhibition assay, they found that lymphocytes harvested from tumor-bearing patients were able on bone marrow cells. 43 Additional murine monoclonal antibodies have been raised to cytoplasmic an-to inhibit in vitro tumor growth. 30,31 This was defined as a tumor specific response subject to blocking by tigens, 44 to human NB cell surface glycoproteins, 45 and specifically to the disialogangliosides GD2 and GD3.…”

A comparative review of the immunobiology of murine neuroblastoma and human neuroblastoma

“…40 Such antibodies would have further diagnostic or therapeutic application if issues regarding receptor specificity and cross-reactivity with neural tissue and normal adrenal tissue were resolved.MoHellstrom et al first used in vitro assays in the study of host antitumor immune response; and with noclonal antibodies of murine origin have been raised to a glycolipid antigen on human NB cells 41,42 but not the colony inhibition assay, they found that lymphocytes harvested from tumor-bearing patients were able on bone marrow cells. 43 Additional murine monoclonal antibodies have been raised to cytoplasmic anto inhibit in vitro tumor growth. 30,31 This was defined as a tumor specific response subject to blocking by tigens, 44 to human NB cell surface glycoproteins, 45 and specifically to the disialogangliosides GD2 and GD3.…”