A molybdenum disulfide/gold nanorod composite-based electrochemical immunosensor for sensitive and quantitative detection of microcystin-LR in environmental samples

Zhang, Yanli; Chen, Meng; Li, Haiyan; Yan, Fuqing; Pang, Pengfei; Wang, Hongbin; Wu, Zhan; Yang, Wenrong

doi:10.1016/j.snb.2017.01.030

Cited by 59 publications

(24 citation statements)

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“…The progress achieved in the field of nanotechnology has strongly influenced the development of immunosensors with improved analytical performance. Various nanomaterials have been explored in the construction of microcystin immunosensors, including metal nanoparticles , graphene , carbon nanotubes , metal nanorods and carbon nanofibers . These nanostructures improve the electron transfer efficiency and increase the surface area available for the immobilization of biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Direct Electrochemical Nano‐immunosensor for Microcystin‐LR in Seawater

et al. 2018

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Microcystins are potent hepatotoxins produced by cyanobacteria, which proliferate in wastewaters with high nutrient content. Due to their high toxicity and potential risk to human health, even at low concentrations, the development of a sensitive and rapid method for the monitoring of microcystin‐LR (MC‐LR) in water samples is of great importance. In this context, a new direct electrochemical nano‐immunosensor for MC‐LR detection using the liquid crystal (E)‐1‐decyl‐4‐[(4‐decyloxyphenyl)diazenyl]pyridinium bromide (Br‐Py) as a redox probe and gold nanoparticles stabilized in bovine serum albumin (AuNP‐BSA) is described herein. The microcystin‐LR antibody (anti‐MC‐LR) was covalently immobilized using N‐(3‐dimethylaminopropyl)‐N‐ethylcarbodiimide hydrochloride (EDC) and N‐hydroxysuccinimide (NHS) on an AuNP‐BSA/BrPy film. The proposed sensor response is based on the inhibition of the Br‐Py electrochemical signal after the specific interaction of MC‐LR with immobilized anti‐MC‐LR on the electrode surface. The electrochemical behavior of the immunosensor was studied by square‐wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS). Under optimized conditions, using SWV and an incubation time of 15 min, the immunosensor exhibits a linear response to MC‐LR concentrations of 0.05 to 500.0 ng mL−1 with a detection limit of 0.05 ng mL−1. The anti‐MC‐LR/AuNP‐BSA/Br‐Py/GCE was successfully applied in the determination of MC‐LR in spiked seawater samples.

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Section: Introductionmentioning

confidence: 99%

Direct Electrochemical Nano‐immunosensor for Microcystin‐LR in Seawater

et al. 2018

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“…The maximum tolerance limit of MC-LR concentration is 1 µg L −1 in different water sources by the WHO provisional guideline. Electrochemical biosensors, including 2D nanomaterial-based amperometric immunosensors, impedimetric aptasensors, and PEC aptasensors, have been extensively employed to detect MCs/MC-LR [143][144][145][146][147][148][149][150][151][152][153][154][155][156][157][158][159]. Li et al have fabricated an electrochemical immunosensor based on GO-AuNP nanocomposites for MC-LR detection in water samples though layer-by-layer alternate electrodeposition of GO and chloroauric acid (HAuCl 4 ) on the GCE surface for 20 cycles [147].…”

Section: Microcystinsmentioning

confidence: 99%

Two-Dimensional Layered Nanomaterial-Based Electrochemical Biosensors for Detecting Microbial Toxins

Jian

et al. 2019

Toxins

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Toxin detection is an important issue in numerous fields, such as agriculture/food safety, environmental monitoring, and homeland security. During the past two decades, nanotechnology has been extensively used to develop various biosensors for achieving fast, sensitive, selective and on-site analysis of toxins. In particular, the two dimensional layered (2D) nanomaterials (such as graphene and transition metal dichalcogenides (TMDs)) and their nanocomposites have been employed as label and/or biosensing transducers to construct electrochemical biosensors for cost-effective detection of toxins with high sensitivity and specificity. This is because the 2D nanomaterials have good electrical conductivity and a large surface area with plenty of active groups for conjugating 2D nanomaterials with the antibodies and/or aptamers of the targeted toxins. Herein, we summarize recent developments in the application of 2D nanomaterial-based electrochemical biosensors for detecting toxins with a particular focus on microbial toxins including bacterial toxins, fungal toxins and algal toxins. The integration of 2D nanomaterials with some existing antibody/aptamer technologies into electrochemical biosensors has led to an unprecedented impact on improving the assaying performance of microbial toxins, and has shown great promise in public health and environmental protection.

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“…However, in electrochemical immunosensors, the antibodies are commonly immobilized on the surface of the electrode by physical absorption, which might lead to non-reversible protein denaturation. 18 This detection limit is also simpler and cheaper.…”

Section: Anti-mc-lr/qd Nanohybrids For Mc-lr Detectionmentioning

confidence: 99%

“…However, in electrochemical immunoassays, antibodies are commonly immobilized on the electrode surface by physical adsorption, which may lead to irreversible protein denaturation, then inhibit the interaction between the antibody and the analytical device. 18 Therefore, it is necessary to develop a method that is sensitive, reliable, and easy to operate.…”

Section: Introductionmentioning

confidence: 99%