A molecularly imprinted sensor based on an electrochemiluminescent membrane for ultratrace doxycycline determination

Abstract

A new molecularly imprinted sensor was developed based on an electroluminescent molecularly imprinted polymer (MIP) membrane and used for doxycycline determination. The MIP was prepared by electropolymerization of pyrogallol doped with alizarin red. An electrochemiluminescence (ECL) signal was produced by the oxidation of the poly-pyrogallol polymer and reaction with alizarin red. The luminescence intensity was enhanced by doxycycline molecules which were re-adsorbed in cavities in MIP due to the energy transfer of the doxycycline oxidized intermediate to alizarin red. The changes of ECL intensities were linear with the concentrations of doxycycline in the range of 2 × 10(-10) to 5 × 10(-8) mol L(-1). The detection limit was 5.17 × 10(-11) mol L(-1). This method was utilized to determine doxycycline residuals in fish muscles with satisfactory results.

Electrochemical Behavior and Voltammetric Determination of Chloramphenicol and Doxycycline Using a Glassy Carbon Electrode Modified with Single‐walled Carbon Nanohorns

“…Analogous to CAP, doxycycline (DOX), one of the broad-spectrum antibiotics categorized as tetracycline, showcases excellent ability to a range of bacteria and widely used to cure human and animals. However, studies have shown that the improper use of DOX produced a residuum in animal-derived food, which would damage the human health and induce resistance of many microorganisms to antibiotics [7,8]. Thus, the innovation of reliable method of high efficiency, convenience, sensitivity for the detection of CAP and DOX residuals is of great importance.…”

“…To date, some techniques have been employed for the identification of CAP and DOX, including high performance liquid chromatography (HPLC) [9,10], capillary electrophoresis [11], enzyme-linked immunosorbent assay [12], fluorescence [13,14], chemiluminescence resonance energy transfer [15], electrochemiluminescence [7,16] and electrochemistry [6,8,[17][18][19][20][21]. Among them, electrochemical method offers the advantages of simplicity, sensitivity and low cost in comparison with other methods.…”

A dual-emission nano-rod MOF equipped with carbon dots for visual detection of doxycycline and sensitive sensing of MnO4−

“…However, the residue of doxycycline in water-based environments and everyday foods has several side effects, such as hepatotoxicity, yellow teeth, gastrointestinal symptoms and mentation changes, especially in children; in addition, doxycycline use can lead to antibiotic resistance. 2,3 Numerous test methods have been developed to detect tetracycline antibiotics: electrochemical analysis, 4,5 UV absorbance spectroscopy (UV-vis), 6,7 liquid chromatography, [8][9][10] ow-injection analysis, 11,12 and uorescence spectrophotometry. 13,14 Fluorescence spectrophotometry stands out among these methods because it is sensitive, fast and cost-effective.…”

Fluorometric determination of doxycycline based on the use of carbon quantum dots incorporated into a molecularly imprinted polymer

“…Another challenge with CQD-based biosensors is the lack of selectivity, which makes it difficult to detect trace amounts of a specific analyte in complicated matrices. In order to improve the specificity, it is of great interest to combine molecularly imprinted polymers (MIPs) with CQDs [15][16][17]. The MIPs are prepared by the polymerization of the functional monomer, cross-linker, and initiator in the presence of a template.…”