A Molecular View on Biomaterials and Dental Stem Cells Interactions: Literature Review

Aguilar-Ayala, Fernando Javier; Aguilar‐Pérez, Fernando Javier; Nic-Can, Geovanny I.; Rojas-Herrera, Rafael; Chuc-Gamboa, Martha Gabriela; Aguilar-Pérez, David Alejandro; Rodas-Junco, Beatriz A.

doi:10.3390/app12125815

Cited by 4 publications

(3 citation statements)

References 110 publications

(143 reference statements)

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“…Several in vitro studies have shown that mechanical stimulation, such as that induced by collagen I hydrogel contraction, induces differentiation of mesenchymal stem/progenitor cells into osteoblasts [41,42], which also explains the high activity of ALP. At the same time, odontoblast differentiation under the influence of mechanical forces is shown in 3D scaffolds only for DPSCs [43][44][45].…”

Section: Discussionmentioning

confidence: 91%

Influence of Extracellular Matrix Components on the Differentiation of Periodontal Ligament Stem Cells in Collagen I Hydrogel

Ivanov,

Kuznetsova,

Popova

et al. 2023

Cells

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Regeneration of periodontal tissues requires an integrated approach to the restoration of the periodontal ligament, cementum, and alveolar bone surrounding the teeth. Current strategies in endogenous regenerative dentistry widely use biomaterials, in particular the decellularized extracellular matrix (dECM), to facilitate the recruitment of populations of resident cells into damaged tissues and stimulate their proliferation and differentiation. The purpose of our study was to evaluate the effect of the exogenous components of the extracellular matrix (hyaluronic acid, laminin, fibronectin) on the differentiation of periodontal ligament stem cells (PDLSCs) cultured with dECM (combinations of decellularized tooth matrices and periodontal ligament) in a 3D collagen I hydrogel. The immunohistochemical expression of various markers in PDLSCs was assessed quantitatively and semi-quantitatively on paraffin sections. The results showed that PDLSCs cultured under these conditions for 14 days exhibited phenotypic characteristics consistent with osteoblast-like and odontoblast-like cells. This potential has been demonstrated by the expression of osteogenic differentiation markers (OC, OPN, ALP) and odontogenic markers (DSPP). This phenomenon corresponds to the in vivo state of the periodontal ligament, in which cells at the interface between bone and cementum tend to differentiate into osteoblasts or cementoblasts. The addition of fibronectin to the dECM most effectively induces the differentiation of PDLSCs into osteoblast-like and odontoblast-like cells under 3D culture conditions. Therefore, this bioengineered construct has a high potential for future use in periodontal tissue regeneration.

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Section: Discussionmentioning

confidence: 91%

Influence of Extracellular Matrix Components on the Differentiation of Periodontal Ligament Stem Cells in Collagen I Hydrogel

Ivanov,

Kuznetsova,

Popova

et al. 2023

Cells

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“… 78 In recent years, the use of fibrin in combination with synthetic or natural polymers has been investigated as a potential solution to this issue. 79 – 81 Other potential solutions include the use of solid scaffolds and their chemical modifications.…”

Section: The Use Of Fibrin Hydrogels In Tissue Engineering: Challengesmentioning

confidence: 99%

Technological advances in fibrin for tissue engineering

et al. 2023

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Fibrin is a promising natural polymer that is widely used for diverse applications, such as hemostatic glue, carrier for drug and cell delivery, and matrix for tissue engineering. Despite the significant advances in the use of fibrin for bioengineering and biomedical applications, some of its characteristics must be improved for suitability for general use. For example, fibrin hydrogels tend to shrink and degrade quickly after polymerization, particularly when they contain embedded cells. In addition, their poor mechanical properties and batch-to-batch variability affect their handling, long-term stability, standardization, and reliability. One of the most widely used approaches to improve their properties has been modification of the structure and composition of fibrin hydrogels. In this review, recent advances in composite fibrin scaffolds, chemically modified fibrin hydrogels, interpenetrated polymer network (IPN) hydrogels composed of fibrin and other synthetic or natural polymers are critically reviewed, focusing on their use for tissue engineering.

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“…Also, the advances in research into the interactions between these biomaterials and the molecular components involved (mechanosensors and mechanotransduction) in DT-MSCs during their proliferation and differentiation were analyzed. The authors concluded that these kinds of biomaterials can contribute to research into regenerative medicine and the development of autologous stem cell therapies [8].…”

mentioning

confidence: 99%