A molecular insight into the role of inflammation in the behavior and pathogenesis of odontogenic cysts

Abstract

Background:Although, odontogenickeratocyst (OKC) and dentigerous cyst (DC) are considered asdevelopmental cysts inflammation has been seen in its connective tissue wall. Inflammation is seen to alter the epithelial lining of both OKC and DC butwhether it plays in altering the behavior of these cysts is not fully understood.Aim:The present study is conducted with the help of molecular marker proliferating cellular nuclear antigen (PCNA) to assess the proliferative activity of OKC and DC and to further evaluate and correlate the effect of inflammation on the proliferative activity and hence on biological behavior of these cysts.Materials and Methods:Immunohistochemicalstaining was performed using anti-PCNA antibody in 10 cases each of classical OKC, inflamed OKC, classical DC, and inflamed DC. The resulting data was tabulated on Microsoft excel and subjected to statistical analysis using two-way analysis of variance test, t test and post-hoc test followed by Bonferroni test with the application of statistical package for social sciences (SPSS) (SPSS, Chicago, IL, USA) and Epi-Info 6.04 d, Atlanta, Georgia (USA).Results:Total mean PCNA expression is statistically higher in inflamed OKC than classical OKC and inflamed DC also showed significantly higher PCNA expression than classical DC (P < 0.001). Correlation between inflammation and PCNA expression was not statistically significant (P ≥ 0.05).Conclusion:Inflammation is responsible for change in behavior of neoplastic epithelium of OKC, whereas in dentigerous it is responsible for changes in epithelial lining and hence should be treated meticulously.

Variability in the Epithelial Expression of Ki67 and Polarization Characteristics of Collagen in Odontogenic Cysts and Tumours

“…We found overall low expression of Ki67 in DC but the presence of inflammation correlated with higher expression of Ki67. A similar result was found by Singh HP et al [9] using PCNA. Although a dentigerous cyst is categorized as a developmental cyst, studies have shown the initial inflammatory response as the initiator of a dentigerous cyst.…”

“…Gadbail et al [3], and Singh et al [9] have also reported a higher suprabasal cell proliferation rate of the Odontogenic Keratocyst. Studies have found that OKC shows higher expression of the anti-apoptotic molecule bcl2 throughout the thickness of the lining epithelium.…”

The Prevalence of EBV and KSHV in Odontogenic Lesions

“…In spite of being classified as developmental lesions, the majority of odontogenic keratocysts and dentigerous cysts are characterised by the presence of chronic inflammatory infiltrate in the connective tissues. 23 Furthermore, the inflammatory reaction is also observed in ameloblastomas. 1 In this study, we used a primer designed to detect EBV-DNA by targeting EBNA-1 segment.…”

Single-Cell RNA Sequencing Reveals CXCLs Enriched Fibroblasts Within Odontogenic Keratocysts

“… 6 Compared to classical OKC and dentigerous cyst (DC), inflamed OKC and inflamed DC showed significantly higher proliferating cell nuclear antigen expression. 7 The thicker lining epithelia was also found in OKC with focal inflammation sites than those without inflammation. 8 Therefore, inflammation might be closely related with the focal proliferation of epithelia.…”