A molecular genetic research of the Triticum sinskajae A. Filat. et Kurk. by RAPD analysis and by comparing the nucleotide sequences of the variable intergenic region of the petN-trnC-GCA chloroplast genome and intron of the histone H3.2 gene

Kuluev, A. R.; Matnijazov, Rustam T.; Kuluev, B. R.; Чемерис, А. В.

doi:10.17816/ecogen16153-59

Cited by 7 publications

(8 citation statements)

References 12 publications

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“…sinskajae is a little-studied diploid wheat, which was previously thought to be formed as a result of a spontaneous mutation of T. monococcum (Filatenko and Kurkiev 2000). Previously, using classical molecular genetic analysis (Kuluev et al 2018), as well as by comparing the nucleotide sequences of plastid genomes (Kuluev et al 2020), we obtained data indicating the existence of a separate species of diploid wheat -T. sinskajae. On the other hand, Sinskaya wheat exhibits complete genetic compatibility with T. monococcum and T. boeoticum (Kurkiev and Filatenko 2000).…”

Section: Discussionmentioning

confidence: 99%

“…These two wheats evolved for a long time in reproductive isolation, but possibly within the same range. Of course, T. sinskajae separated from the general line of diploid wheats or from T. monococcum later than other einkorns, but nevertheless managed to accumulate quite a signi cant number of mutations both in the nuclear (Goncharov et al 2007, Watanabe 2017, Kuluev et al 2018) and in the chloroplast genomes. When deciding on the species identity of T. sinskajae, it may be useful to analyze the similarity of plastid genomes in representatives of the genus Aegilops, in which, despite very close morphological similarity, many species have been identi ed.…”

Section: Discussionmentioning

confidence: 99%

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Sequencing and analysis of complete plastid genomes of diploid wheats Tritcum sinskajae and Triticum monococcum k-20970

Azat,

Bulat,

Alexey

2023

Preprint

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Diploid wheat with genome A Triticum sinskajae A. Filat. et Kurk. was discovered in the early 70s. last century during the reproduction of samples of Triticum monococcum L. line k-20970. Despite the fact that T. sinskajae was initially proposed to be identified as a separate wheat species, in further studies it was considered as a spontaneous mutant of T. monococcum. A number of studies have shown that T. sinskajae is genetically different from different T. monococcum lineages, but how different it is from the T. monococcum k-20970 lineage remained unknown. Based on this, the purpose of this study was to sequence and compare the chloroplast genomes of T. sinskajae and T. monococcum line k-20970, as well as to clarify the phylogenetic relationships between diploid wheats based on the results of analysis of the nucleotide sequences of their chloroplast genomes. During this study, we were the first to sequence and annotate the complete chloroplast genomes of T. sinskajae k-48993 (Dagestan), T. monococcum k-20970 (Turkey), T. boeoticum k-58674 (Armenia) on a Genolab M sequencer (GeneMind, China). For comparative analysis, chloroplast genome sequences of T. monococcum subsp. monococcum (LC005977.1) and T. urartu (KJ614411.1) were taken from GenBank. According to the results of the analysis, it turned out that T. sinskajae (k-48993) and T. monococcum k-20970, although they were closer to each other, differed in 10 changes in the chloroplast genome, including 3 substitutions, 1 transversion, 1 deletion, 4 insertions and 4bp inversion of AGAA with TTCT in the rbcL-psal intergenic region. The identified differences in the chloroplast genomes of T. sinskajae (k-48993) and T. monococcum (k-20970) allow us to conclude that they are two very close, but still different species of diploid wheat. A comparison of the chloroplast genomes of diploid wheats showed a common origin of the plasmons of three closely related einkorns - T. sinskajae, T. monococcum and T. boeoticum, while T. urartu is located a little further from them.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Sequencing and analysis of complete plastid genomes of diploid wheats Tritcum sinskajae and Triticum monococcum k-20970

Azat,

Bulat,

Alexey

2023

Preprint

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“…Such primers enable to amplification of DNA segments that are located quite close to each other in the inter-microsatellite region. ISSR-PCR markers have a dominant inheritance pattern (Selezneva and Akhmedov, 2016;Bardukov et al, 2014;Kuluev et al, 2018).…”

Section: Population-genetic Analysis Of Speciesmentioning

confidence: 99%

Genetic Diversity of Common Wild Wheat Species in Armenia Using ISSR Markers

Badalyan,

Aloyan,

Dilanyan

et al. 2023

OnLine Journal of Biological Sciences

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Armenia is one of the primary genealogical centers of numerous crops, the wild relatives of many of which are still found in Armenia today. Population limits of three out of 4 species of wild relatives of endemic wheat in Armenia (T. araraticum Jakubz, T. urartu Tumanian ex Gandilyan, T. boeoticum Boiss.) have been reduced in recent decades as a result of various ecological and economic functions, they are facing the danger of extinction and are registered in the Red Book of Plants of the Republic of Armenia. In order to identify these species as selection raw material, genetic characterization of populations was carried out according to ISSR-PCR markers, as well as decoding genetic formulas and barcodes. As a result, according to the coefficients of the genetic pattern, the studied wild types of wheat are characterized as basic or typical gene pools with the least quantity and frequency of rare alleles. The populations are stable and in conditions of efficient number of isolates, the gene pool is capable of self-reproduction. The obtained results as genetic markers will provide an opportunity to further clarify the origin of the species, evolution, patterns of inheritance of useful economic traits, cell genetic characteristics of the species, natural resistance to adverse environmental factors and diseases, etc.

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“…is the youngest taxon discovered in the accession K-20970 of cultivated einkorn wheat in the second half of 20th century (Filatenko, Kurkiev, 1975). In Russia, T. sinskajae is represented by a single accession K-48993, in many studies (Goncharov et al, 2007;Kuluev et al, 2018). The authors stated that this species was a natural naked mutant of hulled diploid T. monococcum.…”

Section: Introductionmentioning

confidence: 99%

“…To develop early, freethreshing and semi-dwarf einkorn lines, Watanabe (2017) utilised two different genes determining free threshing of einkorn wheat, one sog (soft glume) of T. sinskajae and another sos (soft spike) of T. monococcum. Kuluev et al (2018) found differences between T. sinskajae and T. monococcum in the variable region of the histone gene H3.2, and the random amplified polymorphic DNA (RAPD) analysis showed the presence of unique polymorphic loci in T. sinskajae.…”

Section: Introductionmentioning

confidence: 99%

Characterisation of synthetic hexaploid wheat (BBAuAuAmAm) and its potential use in wheat breeding

Daskalova

Doneva

Stanoeva

et al. 2022

Zemdirbyste-Agriculture

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Free-threshing einkorn wheat (Triticum sinskajae A. Filat. et Kurk.) is an excellent source of resistance against wheat diseases and grain quality parameters. A new synthetic hexaploid amphiploid SHW45 (T. durum 198-2 × Triticum sinskajae acc. 18397) was studied in C 2-7 generations and characterised for field growing. Some traits such as a plant height and spike features including fertility and spike fragility revealed a large phenotypic variation. A strong selection for resistance to spikelet dispersal and a number of seeds per selfed spike led to the creation of several lines. They manifested a variety of resistance to powdery mildew at the seedling stage. Three amphiploid lines with diverse phenotypes and a complete resistance to spike brittleness were crossed as females to the wheat cultivar 'Sadovska ranozreika-4'. Viable hybrid seeds are obtained only when the synthetic form is a female parent. F 1 hybrids of amphiploid line 45-10 bore an average of 9 seeds per isolated spike, which was a significantly better yield than that of other hybrids. The lines showed homogeneity in high molecular weight (HMW) glutenins, as subunit pairs Ax2*+Ay1 and Bx17+By18 are inherited from the T. sinskajae, and T. durum form 198-2, respectively. In total, 14 F 2 , 22 BC 1 , and 141 F 2 -BC 1 seeds from the crosses with two bread wheat cultivars were planted in the field. The synthetic amphiploid lines might be promising genetic resources for resistance to powdery mildew and grain quality in wheat breeding.

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A molecular genetic research of the Triticum sinskajae A. Filat. et Kurk. by RAPD analysis and by comparing the nucleotide sequences of the variable intergenic region of the petN-trnC-GCA chloroplast genome and intron of the histone H3.2 gene

Cited by 7 publications

References 12 publications

Sequencing and analysis of complete plastid genomes of diploid wheats Tritcum sinskajae and Triticum monococcum k-20970

Sequencing and analysis of complete plastid genomes of diploid wheats Tritcum sinskajae and Triticum monococcum k-20970

Genetic Diversity of Common Wild Wheat Species in Armenia Using ISSR Markers

Characterisation of synthetic hexaploid wheat (BBAuAuAmAm) and its potential use in wheat breeding

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