A molecular forensic method for identifying species composition of processed marine mammal meats

Chang, Chia‐Hao; Yao, Chiou-Ju; Yu, Hon‐Tsen; Liao, Yun-Chih; Jang-Liaw, Nian-Hong; Tsai, Chi-Li; Shao, Kwang‐Tsao

doi:10.1016/j.jflm.2014.01.012

Cited by 6 publications

(2 citation statements)

References 23 publications

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“…For example, DNA extraction is still workable from samples that have undergone food processing or digestion (Chang et al . , , ; Galal‐Khallaf et al . ; Long et al .…”

Section: Introductionmentioning

confidence: 99%

DNA barcodes of the native ray‐finned fishes in Taiwan

Chang

Shao

Lin

et al. 2016

Molecular Ecology Resources

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Species identification based on the DNA sequence of a fragment of the cytochrome c oxidase subunit I gene in the mitochondrial genome, DNA barcoding, is widely applied to assist in sustainable exploitation of fish resources and the protection of fish biodiversity. The aim of this study was to establish a reliable barcoding reference database of the native ray-finned fishes in Taiwan. A total of 2993 individuals, belonging to 1245 species within 637 genera, 184 families and 29 orders of ray-finned fishes and representing approximately 40% of the recorded ray-finned fishes in Taiwan, were PCR amplified at the barcode region and bidirectionally sequenced. The mean length of the 2993 barcodes is 549 bp. Mean congeneric K2P distance (15.24%) is approximately 10-fold higher than the mean conspecific one (1.51%), but approximately 1.4-fold less than the mean genetic distance between families (20.80%). The Barcode Index Number (BIN) discordance report shows that 2993 specimens represent 1275 BINs and, among them, 86 BINs are singletons, 570 BINs are taxonomically concordant, and the other 619 BINs are taxonomically discordant. Barcode gap analysis also revealed that more than 90% of the collected fishes in this study can be discriminated by DNA barcoding. Overall, the barcoding reference database established by this study reveals the need for taxonomic revisions and voucher specimen rechecks, in addition to assisting in the management of Taiwan's fish resources and diversity.

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“…For example, DNA extraction is still workable from samples that have undergone food processing or digestion (Chang et al . , , ; Galal‐Khallaf et al . ; Long et al .…”

Section: Introductionmentioning

confidence: 99%

DNA barcodes of the native ray‐finned fishes in Taiwan

Chang

Shao

Lin

et al. 2016

Molecular Ecology Resources

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“…Despite the vast application of the DNA Barcode method for animal identification, focused on the Folmer region, this method is not universally efficient for cetaceans. This method has been efficient for identification of several cetacean species (Tsai et al, 2013;Chang et al, 2014;Cypriano-Souza et al, 2016). However, there have been cases in which the Folmer region was not useful for differentiating Delphinus delphis, D. capensis, Stenella coeruleoalba, S. frontalis, and Tursiops truncatus, all of which are species in the family Delphinidae, more specifically in the subfamily Delphininae (Amaral et al, 2007;Viricel and Rosel, 2012;Alfonsi et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Molecular identification of cetaceans from the West Atlantic using the E3-I5 region of COI

Falcão¹,

Campos

Freitas

et al. 2017

Genet. Mol. Res.

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Molecular identification is very useful in cases where morphology-based species identification is not possible. Examples for its application in cetaceans include the identification of carcasses of stranded animals in advanced state of decomposition and body parts that are illegally traded. One DNA region that is often used for molecular identification is the Folmer region of the mitochondrial gene cytochrome c oxidase subunit I (COI) (locus 48 to 705 bp). This locus has been used for the identification of several animal species, including whales and dolphins. The goal of the present study was to evaluate the usefulness of another region of COI, the E3-I5 (locus 685 to locus 1179; 495 bp) as a marker for identification of cetaceans from northeastern Canada and northeastern Brazil. The identification markers were successfully obtained for seven cetacean species after performing percent identity and Basic Local Alignment Search Tool analyses. The obtained markers are now publicly available and are useful for the identification of the endangered blue whale (Balaenoptera musculus), common minke whale (B. acutorostrata), vulnerable sperm whale (Physeter macrocephalus), harbor porpoise (Phocoena phocoena), common bottlenose dolphin (Tursiops truncatus), Guiana dolphin (Sotalia guianensis), and melon-headed whale (Peponocephala electra).

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