Molecular identification of cetaceans from the West Atlantic using the E3-I5 region of COI

Falcão, Luara Hanna Oliveira; Campos, A. S.; Freitas, João Eduardo Pereira de; Furtado-Neto, M.A.A.; Faria, Vicente Vieira

doi:10.4238/gmr16029607

Cited by 4 publications

(4 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BLAST is an efficient method of determining similarities and dissimilarities of sequences that are available in an online database and can be used to confirm species when morphological appearances are not useful [39,40]. The percent identity of the D-loop sample from our unknown whale agreed with data from the cranium morphology, as it had a high value of similarity to the D-loop sequence of B. acutorostrata but not B. omurai.…”

Section: Discussionmentioning

confidence: 61%

First Stranding Event of a Common Minke Whale (Balaenoptera acutorostrata Lacépède, 1804) Reported in the Gulf of Thailand

et al. 2023

View full text Add to dashboard Cite

On 5 September 2022, a dead baleen whale was found stranded at Laem Phak Bia, Phetchaburi, the Gulf of Thailand, Thailand but could not be identified because it was in an advanced stage of decomposition. It was first suspected to be Omura’s whale (Balaenoptera Omurai), as that is a common species in the Gulf of Thailand. However, the cranium morphology was different from B. omurai and more similar to the common minke whale (Balaenoptera acutorostrata) from the North Pacific Ocean, which has never been reported in Thai territorial waters. The mitochondrial DNA control region (D-loop) was then used to identify the species through the Basic Local Alignment Search Tool (BLAST) available at the National Center for Biotechnology Information (NCBI) GenBank, which resulted in a high percent identity, 96.49 to 98.84, with B. acutorostrata. A Bayesian phylogenetic tree was further used to confirm the species, which grouped with B. acutorostrata from the North Pacific Ocean. This study provides evidence of the first stranding event of B. acutorostrata in the Gulf of Thailand. It is new information that extends previous knowledge on the distribution of the common minke whale and raises the need for more active surveys of cetaceans in the South China Sea going forward.

show abstract

Section: Discussionmentioning

confidence: 61%

First Stranding Event of a Common Minke Whale (Balaenoptera acutorostrata Lacépède, 1804) Reported in the Gulf of Thailand

et al. 2023

View full text Add to dashboard Cite

show abstract

“…An examination of the mtDNA gene sequence data obtained from pairs of closely-related cetacean populations, subspecies and species found that the determined subspecies-level was different from the actual subspecies pairs ( Rosel et al, 2017 ). On the other hand, several studies demonstrated that the COX-I or DNA barcoding technique allowed for accurate identification of some species such as Blue whale ( Balaenoptera musculus ), Pygmy, Dwarf and Sperm whales ( Physeter macrocephalus ), common bottlenose dolphin ( Tursiops truncates ), Fraser’s dolphin, Risso’s dolphin, Striped dolphin, and Cuvier’s beaked whale ( Alfonsi et al, 2013 ; Amaral, Sequeira & Coelho, 2007 ; Falcao et al, 2017 ; Silva et al, 2021 ; Viricel & Rosel, 2012 ). COX-I has been used in the identification of stranded rare species or deep-living species from highly degraded carcasses that could otherwise not be used to identify animals via their external morphology ( Thompson et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Feasibility of melting fingerprint obtained from ISSR-HRM curves for marine mammal species identification

Kriangwanich

Buddhachat

Poommouang

et al. 2021

PeerJ

View full text Add to dashboard Cite

Currently, species identification of stranded marine mammals mostly relies on morphological features, which has inherent challenges. The use of genetic information for marine mammal species identification remains limited, therefore, new approaches that can contribute to a better monitoring of stranded species are needed. In that context, the ISSR-HRM method we have proposed offers a new approach for marine mammal species identification. Consequently, new approaches need to be developed to identify individuals at the species level. Eight primers of the ISSR markers were chosen for HRM analysis resulting in ranges of accuracy of 56.78–75.50% and 52.14–75.93% in terms of precision, while a degree of sensitivity of more than 80% was recorded when each single primer was used. The ISSR-HRM primer combinations revealed a success rate of 100% in terms of discrimination for all marine mammals included in this study. Furthermore, ISSR-HRM analysis was successfully employed in determining marine mammal discrimination among varying marine mammal species. Thus, ISSR-HRM analysis could serve as an effective alternative tool in the species identification process. This option would offer researchers a heightened level of convenience in terms of its performance and success rate. It would also offer field practice to veterinarians, biologists and other field-related people a greater degree of ease with which they could interpret results when effectively classifying stranded marine mammals. However, further studies with more samples and with a broader geographical scope will be required involving distinct populations to account for the high degree of intraspecific variability in cetaceans and to demonstrate the range of applications of this approach.

show abstract

“…Since 2012, three new studies evaluated the using of DNA barcoding to identify cetacean species along the coasts of South America. Falcão et al (2017) evaluated the usefulness of an additional region of the COI gene -the E3-I5 region (loci 685 to 1179; 495 bp) -as a marker for identification of cetaceans from northeastern Canada and northeastern Brazil. The study encompassed seven species, including samples of four species collected in Brazilian waters: Physeter macrocephalus, Peponocephala electra, Sotalia guianensis, and Tursiops truncatus.…”

Section: Barcoding In Latin American Aquatic Mammalsmentioning

confidence: 99%

Latin American aquatic mammals: an overview of 12 years focusing on molecular techniques applied to conservation

Castro

Caballero

Cunha

et al. 2023

Lat. Am. J. Aquat. Mamm.

View full text Add to dashboard Cite

Ecological information useful for conservation purposes have benefitted from recent and rapid advancements in genetic techniques, revealing unknown aspects of behavior, natural history, population structure and demography of several aquatic mammal species, many of them with conservation concerns. Molecular markers have been used to define management units, to settle taxonomic uncertainties, to control illegal wildlife trade, among others, providing valuable information to decision-making to conserve and manage aquatic mammals. We review genetic studies applied to conservation-related issues involving natural populations of more than 40 species of aquatic mammals in Latin America, covering four taxonomic groups. The main goal was to assess which genetic approaches have been used and to identify gaps in genetic research relating to geographic areas and species. We reviewed studies published in peer-reviewed journals between 2011 and 2022, and found that most were focused on population structure, phylogeography, gene flow and dispersal movements. The review revealed that researchers need to increase and improve the knowledge in those species which face major conservation concern. Scarce findings were related to forensics and its application to wildlife trade. In the era of next-generation-sequencing techniques, just a few studies used genomics as a tool for monitoring gene diversity, an important goal to help us predict how species will cope with climate change events. Looking to the future we suggest which species, geographic areas and genetic studies should be prioritized in a scenario of climate change and increased human threats (e.g., fishery bycatch, habitat degradation, etc.) and the urgent need for conservation actions. Finally, we highlight the benefits of the collaborative works and the necessity of generating a conservation genetic network, with an open agenda to discuss the local and regional problematics. All in all, we strongly emphasize the generation of critical information towards the effective conservation and management of aquatic mammals in Latin America.

show abstract

Molecular identification of cetaceans from the West Atlantic using the E3-I5 region of COI

Cited by 4 publications

References 32 publications

First Stranding Event of a Common Minke Whale (Balaenoptera acutorostrata Lacépède, 1804) Reported in the Gulf of Thailand

First Stranding Event of a Common Minke Whale (Balaenoptera acutorostrata Lacépède, 1804) Reported in the Gulf of Thailand

Feasibility of melting fingerprint obtained from ISSR-HRM curves for marine mammal species identification

Latin American aquatic mammals: an overview of 12 years focusing on molecular techniques applied to conservation

Contact Info

Product

Resources

About