A Molecular Cytogenetic Map of Scallop (Patinopecten yessoensis)

Yang, Zujing; Li, Xuan; Liao, Huan; Hu, Liping; Cheng, Peng; Wang, Shenhai; Huang, Xiaoting; Bao, Zhenmin

doi:10.1007/s10126-019-09918-6

Cited by 6 publications

(6 citation statements)

References 36 publications

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“…In many plant species, the FISH mapping of BAC clones containing amplified fragment length polymorphism (AFLP) or microsatellite genetic markers accomplished the recognition of chromosomes as well as the integration of linkage groups and chromosomes (Kim et al, 2002(Kim et al, , 2005Tang et al, 2009;Yurkevich et al, 2021). Similar research was also reported in the scallop species of P. yessoensis, and the 19 LGs were successfully assigned to 19 pairs of chromosomes by SNP-anchored fosmid clones, which achieving chromosome recognition and integration of genetic and cytogenetic map (Yang et al, 2019). In addition, the distributions of microsatellite markers in some plant species were observed that they were specific and correlated with certain chromosomal organizations (e.g., euchromatin, heterochromatin, and centromeres) (Cuadrado et al, 2008).…”

Section: Discussionmentioning

confidence: 68%

“…In white campion Silene latifolia, 12 pairs of chromosomes were distinguished by the simultaneous hybridization of five BAC clones and three repetitive sequences on mitotic chromosomes (Lengerova et al, 2004). Furthermore, based on FISH, the integration of genetic and cytogenetic maps was carried out in many organisms, such as Bombyx mori (Yoshido et al, 2005), C. farreri (Feng et al, 2014), Scophthalmus maximus (Taboada et al, 2014), P. yessoensis (Yang et al, 2019), and some plants (Wang et al, 2006;Xiong et al, 2010;Dong et al, 2018;Yurkevich et al, 2021), which assisted the chromosome identification and evolution researches in the abovementioned species.…”

Section: Introductionmentioning

confidence: 99%

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Chromosome Identification and Cytogenetic Map Construction of Zhikong Scallop (Chlamys farreri) Based on Fluorescence in situ Hybridization

Jiang

Xing

et al. 2021

Front. Mar. Sci.

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Zhikong scallop (Chlamys farreri) is a bivalve species with broad economic and biological value, and an essential species of aquaculture in North China. Recently, efforts have been made to improve knowledge of genome, genetics, and cytogenetics, which is devoted to develop the molecular breeding project for the scallop. In this study, we constructed a cytogenetic map and identified all chromosomes of C. farreri using fluorescence in situ hybridization (FISH). A total of 100 Bacterial Artificial Chromosome (BAC) clones and 27 fosmid clones, including 58 microsatellite marker-anchored BAC clones, 4 genes-anchored BAC clones, 38 random BAC clones, 22 repetitive sequences-anchored fosmid clones, and 5 gene-anchored fosmid clones, were tested as probes, and 69 of them produced specific and stable signal on one pair of chromosomes. Then, multiple co-hybridizations were conducted to distinguish all the submetacentric and subtelocentric chromosomes with similar morphology by the abovementioned chromosome-specific markers. On this basis, a cytogenetic map of C. farreri containing 69 clones was constructed by co-hybridization and karyotype analysis. The markers covered all 19 pairs of chromosomes, and the average number of markers on each chromosome was 3.6. The cytogenetic map provides a platform for genetic and genomic analysis of C. farreri, which facilitates the molecular breeding project of C. farreri and promotes the comparative studies of chromosome evolution in scallops and even bivalves.

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Section: Discussionmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Chromosome Identification and Cytogenetic Map Construction of Zhikong Scallop (Chlamys farreri) Based on Fluorescence in situ Hybridization

Jiang

Xing

et al. 2021

Front. Mar. Sci.

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“…It was possible to obtain chromosomal markers for all the chromosomes of Senegalese sole. Chromosomal markers have been used for various applications; for example, the integration of cytogenetic and genomic data in different species of cichlids allowed the detection of large conserved synthetic regions [ 43 ], or the consolidation of the genetic, physical and cytogenetic maps, in the scallop species Patinopecten yassinoensis [ 44 ]. In the specific case of flatfish, it could be an additional tool to solve the controversial taxonomy of the group.…”

Section: Discussionmentioning

confidence: 99%

A Comprehensive Integrated Genetic Map of the Complete Karyotype of Solea senegalensis (Kaup 1858)

Merlo

Portela-Bens

Rodríguez

et al. 2020

Genes

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Solea senegalensis aquaculture production has experienced a great increase in the last decade and, consequently, the genome knowledge of the species is gaining attention. In this sense, obtaining a high-density genome mapping of the species could offer clues to the aquaculture improvement in those aspects not resolved so far. In the present article, a review and new processed data have allowed to obtain a high-density BAC-based cytogenetic map of S. senegalensis beside the analysis of the sequences of such BAC clones to achieve integrative data. A total of 93 BAC clones were used to localize the chromosome complement of the species and 588 genes were annotated, thus almost reaching the 2.5% of the S. senegalensis genome sequences. As a result, important data about its genome organization and evolution were obtained, such as the lesser gene density of the large metacentric pair compared with the other metacentric chromosomes, which supports the theory of a sex proto-chromosome pair. In addition, chromosomes with a high number of linked genes that are conserved, even in distant species, were detected. This kind of result widens the knowledge of this species’ chromosome dynamics and evolution.

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“…FISH with chromosome-specific probes can provide abundant and reliable markers for cytogenetic researches (Stein et al, 2001;Yang et al, 2019), including cytogenetic map (Pinkel et al, 1986;Phillips et al, 2006b), chromosome identification (Stein et al, 2001;Yang et al, 2019), chromosome rearrangement (Phillips et al, 2003;Phillips et al, 2013;Bielski et al, 2020), and chromosome evolution across species (Phillips et al, 2001;Ross et al, 2009;do Vale Martins et al, 2021). Chromosome-specific FISH probes have been developed by several methods, such as DOP-PCR after microdissection or flow sorting (Telenius et al, 1992;Vandewoestyne et al, 2009), and screening BACs corresponding to specific linkage groups from a constructed library (Wang et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Development and applications of chromosome-specific cytogenetic BAC-FISH probes in Larimichthys crocea

Zhang

Xie²,

Liu³

et al. 2022

Front. Mar. Sci.

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Large yellow croaker Larimichthys crocea (Richardson) is an important member in family Sciaenidae, and one of most productive mariculture fishes in China. Fluorescence in situ hybridization is a useful tool for cytogenetics and genomics research. Here, we demonstrated that bacterial artificial chromosome (BAC) clones could be used to identify individual chromosomes in large yellow croaker, and also to study chromosome evolution in the related species. By using BAC paired-end sequencing and sequence alignment, 435 BAC clones were anchored to 24 pseudochromosomes of large yellow croaker genome. Among them, 72 BAC clones with low repeat content were selected and passed PCR test, and then grouped by chromosome for FISH test. As a results, there were 67 BAC clones, 2 to 3 BAC clones per chromosome, generated specific and stable signal at expected position. Then, a dual-color FISH probe cocktail composed of 48 of these BAC clones was formulated and used to hybridize metaphase chromosome spreads, resulting in distinct signal patterns on each chromosome pair, which help to distinguish all chromosomes in the metaphase spreads of large yellow croaker. The chromosome-specific BAC-FISH probes were also applied to a close relative of large yellow croaker, Collichthys lucidus, demonstrating that its Y chromosome originated from the fusion of Chr.1 and Chr.7. Thus, our study provides the first set of chromosome-specific FISH probes in family Sciaenidae, which will play an important role in cytogenetics and genomics research in the family.

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A Molecular Cytogenetic Map of Scallop (Patinopecten yessoensis)

Cited by 6 publications

References 36 publications

Chromosome Identification and Cytogenetic Map Construction of Zhikong Scallop (Chlamys farreri) Based on Fluorescence in situ Hybridization

Chromosome Identification and Cytogenetic Map Construction of Zhikong Scallop (Chlamys farreri) Based on Fluorescence in situ Hybridization

A Comprehensive Integrated Genetic Map of the Complete Karyotype of Solea senegalensis (Kaup 1858)

Development and applications of chromosome-specific cytogenetic BAC-FISH probes in Larimichthys crocea

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