Development and applications of chromosome-specific cytogenetic BAC-FISH probes in Larimichthys crocea

Zhang, Jianpeng; Xie, Jingyi; Liu, Bo; Wang, Zhiyong; Wang, Yilei; Jiang, Yonghua; Zhang, Jing; Cai, Mingyi

doi:10.3389/fmars.2022.1078110

Cited by 2 publications

(5 citation statements)

References 59 publications

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“…As expected, the peak ratio of Sr/Ca in each marked group exceeded the detection baseline (1.16 mmol/mol) after 7 days of immersion, indicating a 100% marking success rate. The high values of Sr/Ca ratio within 0.1mm from the core of otolith were found in most individuals, such as Larimichthys crocea, which may be caused by the maternal effect of the parental body [37].…”

Section: Quantitative Analysis Of Otolith Markingmentioning

confidence: 93%

Evaluation of the Effectiveness of Marking Juvenile Takifugu obscurus Otoliths with Strontium

Zhang

Feng

et al. 2022

Fishes

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Strontium marking technology is commonly used for extensive marking in stock enhancement and releasing activities. In order to explore the feasibility of this technology for juvenile Takifugu obscurus, five different concentrations of strontium (0, 12, 18, 36, and 72 mg/L) were set up by strontium chloride hexahydrate (SrCl2·6H2O) and T. obscurus were immersed for 7 days. Then, T. obscurus were reared in non-additive water for 25 days. The results showed that the survival rate of all groups was 100%, except the 72 mg/L group, after 7 days of immersion. Moreover, the growths of all the marked groups were better than that of the control group. There was no significant difference between the control and marked groups, except for the 18 mg/L group, indicating that the appropriate concentration of strontium might have positive effect for T. obscurus. The strontium to calcium (Sr/Ca) ratios of otoliths in the marked groups increased with increasing concentration and time, which were higher than the baseline, respectively. Then, the Sr/Ca ratios returned to the original level, but the peak value was still retained, suggesting that the success rate of the strontium marking reached 100%. Notably, the residual strontium content of 18 mg/L group was insufficient for causing negative effects for T. obscurus after 25 days. Therefore, in consideration of the growth, survival, and effect of strontium marking on otolith, 18 mg/L is an appropriate concentration for strontium marking of juvenile T. obscurus. This study confirmed the feasibility of strontium marking for T. obscurus and provided a new approach to rationally and scientifically evaluate the stock enhancement and releasing efficiency of T. obscurus.

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Section: Quantitative Analysis Of Otolith Markingmentioning

confidence: 93%

Evaluation of the Effectiveness of Marking Juvenile Takifugu obscurus Otoliths with Strontium

Zhang

Feng

et al. 2022

Fishes

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“…The construction of the H. discus hannai BAC library followed previously published methods (Luo et al, 2006;Zhang et al, 2022). Firstly, High molecular genomic DNA was extracted from the adductor muscle of H. discus hannai and digested with HindIII restriction enzyme into 110 -220 kb.…”

Section: Construction and Quality Evaluation Of Bac Librarymentioning

confidence: 99%

“…The methodology was based on previous studies (Zhang et al, 2022). A total of 1044 BAC clones were selected randomly from the BAC library of H. discus hannai.…”

Section: Paired End Sequencing and In Silico Mapping Of Bacsmentioning

confidence: 99%

“…Refer to previous methods for validating candidate clones (Zhang et al, 2022). PCR method was used to verify the correspondence between BAC clones and pseudochromosomes.…”

Section: Validation Of Candidate Chromosomespecific Bac Clonesmentioning

confidence: 99%

“…The exposed slide was visualized using an Olympus epifluorescence microscope equipped with a CCD camera, capturing gray-scale images for each color channel through a digital image capture system (Olympus DP 80). Background subtraction and image feature intensification were carried out following the previous studies (Zhang et al, 2022). And the chromosome pairing and alignment were performed in Adobe photoshop CC 2017.…”

Section: Validation Of Candidate Chromosomespecific Bac Clonesmentioning

confidence: 99%

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Development and applications of chromosome-specific BAC-FISH probes in Pacific abalone (Haliotis discus hannai)

Zhang,

Wang,

Huang

et al. 2023

Front. Mar. Sci.

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Pacific abalone (Haliotis discus hannai) is an economically important marine shellfish for aquaculture and is distributed throughout eastern Asia. Although a lot of genetic breeding work has been carried out, chromosome identification in abalone is still a challenging task. Here, we developed a set of BACs to be chromosome-specific probes in Pacific abalone, and to study chromosome evolution in the related species. Through BAC paired-end sequencing and sequence alignment, we were able to in silico anchor 168 BACs onto 18 pseudochromosomes of Pacific abalone genome. After selecting 42 BACs that contained DNA inserts with minimal repetitive sequences, we validated them through PCR and Fluorescence in situ hybridization (FISH) test. As a result, We obtained specific FISH signals for 26 clones on the chromosomes of Pacific abalone with at least one BAC mapped per chromosome. We also applied the chromosome-specific BAC-FISH probes to a close relative of Pacific abalone, Xishi abalone (H. gigantea), which revealed that chromosome 13 and 15 between the two species underwent a chromosomes rearrangement event. This study provides the first set of chromosome-specific probes for the family Haliotidae, which can serve as an important tool for future cytogenetics and genomics research.

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Development and applications of chromosome-specific cytogenetic BAC-FISH probes in Larimichthys crocea

Cited by 2 publications

References 59 publications

Evaluation of the Effectiveness of Marking Juvenile Takifugu obscurus Otoliths with Strontium

Evaluation of the Effectiveness of Marking Juvenile Takifugu obscurus Otoliths with Strontium

Development and applications of chromosome-specific BAC-FISH probes in Pacific abalone (Haliotis discus hannai)

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