A Molecular and Cellular Context-Dependent Role for Ir76b in Detection of Amino Acid Taste

Ganguly, Anindya; Pang, Lisa Y.; Duong, Vi-Khoi; Lee, Angelina; Schoniger, Hanni; Varady, Erika; Dahanukar, Anupama

doi:10.1016/j.celrep.2016.12.071

Cited by 156 publications

(193 citation statements)

References 44 publications

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“…Thus, both classes of receptors are likely to contribute to responses of Gr / Ir -expressing neurons in the LSO and VCSO, but whether they interact functionally or act independently remains to be determined. In the LSO, expression of sweet Grs and Ir76b overlaps in pharyngeal sweet GRNs, as observed in tarsi as well (Ganguly et al, 2017). In the pharynx, we also found co-expression of ppk28 with Ir genes, which has not been described for external GRNs.…”

Section: Discussionmentioning

confidence: 90%

“…The following fly lines were used: MJ94-GAL4 was a gift from L. Griffith at Brandeis University, Gr-GAL4 (Ling et al, 2014, Weiss et al, 2011), Gr66a-GAL4 (BDSC#28801), Ir-GAL4 (Koh et al, 2014), Ir11a-GAL4 (BDSC#41742), Ir100a-GAL4 (BDSC#41743), Ir76b-GAL4 (BDSC#41730), Ir25a-GAL4 (BDSC#41728), ppk28-GAL4 (Cameron et al, 2010), Gr43a-LexA (Miyamoto and Amrein, 2014), Gr32a-LexA (Fan et al, 2013), Ir76b-LexA (Ganguly et al, 2017), ppk28-LexA (Thistle et al, 2012) , UAS-Kir2.1 (Baines et al, 2001), UAS-VR1 E600K (Marella et al, 2006), poxn ΔM22-B5 (Boll and Noll, 2002), poxn 70 (Awasaki and Kimura, 1997), UAS-mCD8-GFP (Weiss et al, 2011), and LexAop2-6XmCherry-HA (BDSC#52271, 52272). For experiments using poxn mutants, we confirmed the poxn mutant background in all sorted flies by observing the transformed long and bent mechanosensory hairs in the labellum, as well as the fused three tarsal segments in the legs.…”

Section: Methodsmentioning

confidence: 99%

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Molecular and Cellular Organization of Taste Neurons in Adult Drosophila Pharynx

Chen

Dahanukar

2017

Cell Reports

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SUMMARY The Drosophila pharyngeal taste organs are poorly characterized despite their location at important sites for monitoring food quality. Functional analysis of pharyngeal neurons has been hindered by the paucity of molecular tools to manipulate them, as well as their relative inaccessibility for neurophysiological investigations. Here, we generate receptor-to-neuron maps of all three pharyngeal taste organs by performing a comprehensive chemoreceptor-GAL4/LexA expression analysis. The organization of pharyngeal neurons reveals similarities and distinctions in receptor repertoires and neuronal groupings compared to external taste neurons. We validate the mapping results by pinpointing a single pharyngeal neuron required for feeding avoidance of L-canavanine. Inducible activation of pharyngeal taste neurons reveals functional differences between external and internal taste neurons and functional subdivision within pharyngeal sweet neurons. Our results provide road maps of pharyngeal taste organs in an insect model system for probing the role of these understudied neurons in controlling feeding behaviors.

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Section: Discussionmentioning

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Molecular and Cellular Organization of Taste Neurons in Adult Drosophila Pharynx

Chen

Dahanukar

2017

Cell Reports

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“…The division of bitter responses may derive primarily from the segregation of Gr receptors, which showed a similar division into S-a and S-b classes. There is evidence that amino acid responses depend on certain IR receptors (Croset et al , 2016; Ganguly et al , 2017), which may be distributed among sensilla independently of Gr receptors; it will be interesting to carry out an extensive analysis of electrophysiological responses to amino acids in the labella of IR mutants. In any case, the differences among individual sensilla in their response profiles to different amino acids suggests that they may also vary in the amino acid receptors they contain.…”

Section: Discussionmentioning

confidence: 99%

“…We note that responses in the CAFÉ assay are likely to be influenced not only by the labellar sensilla, but by other sensilla as well, including pharyngeal sensilla and perhaps leg sensilla. It would also be interesting to explore the behavioral response to tryptophan as a function of the internal state of the animal; the internal state has been found to influence the behavioral response to other amino acids (Toshima et al , 2012; Ganguly et al , 2017). We note finally that behavioral responses to complex mixtures of amino acids may be stronger than responses to individual amino acids.…”

Section: Discussionmentioning

confidence: 99%

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Physiological responses of theDrosophilalabellum to amino acids

Park

Carlson

2017

Journal of Neurogenetics

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We have systematically studied the physiological responses elicited by amino acids from the principal taste organ of the Drosophila head. Although the detection and coding of sugars and bitter compounds have been examined extensively in this organism, little attention has been paid to the physiology of amino acid taste. We find that one class of sensilla, the S sensilla, yield the strongest responses to amino acids, although these responses were much weaker than the most robust responses to sugar or bitter compounds. S sensilla are heterogeneous in their amino acid responses, and amino acids differ in the responses they elicit from individual sensilla. Tryptophan elicited relatively strong responses from S sensilla, and these responses were eliminated when bitter-sensing neurons were ablated. Although tryptophan yielded little if any response in a behavioral paradigm, phenylalanine elicited a relatively strong response in the same paradigm and had a different physiological profile, supporting the notion that different amino acids are differentially encoded by the repertoire of taste neurons.

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The roles of enteroendocrine cell distribution and gustatory receptor expression in regulating peptide hormone secretion in the midgut of Bombyx mori larvae

Toyam,

Yamagishi,

Sato

2023

Arch Insect Biochem Physiol

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To regulate physiological homeostasis and behavior in Bombyx mori, more than 20 peptide hormones in the midgut of larvae are secreted upon detection of food substances at the lumen. Although it is logical to assume that the timings of peptide hormone secretions are regulated, little is known about the mechanisms. In this study, the distributions of enteroendocrine cells (EECs) producing five peptide hormones and EECs expressing gustatory receptors (Grs), as candidate receptors for luminal food substances and nutrients, were examined via immunostaining in B. mori larvae. Three patterns of peptide hormone distribution were observed. Tachykinin (Tk)‐ and K5‐producing EECs were located throughout the midgut; myosuppressin‐producing EECs were located in the middle‐to‐posterior midgut; and allatostatin C‐ and CCHamide‐2‐producing EECs were located in the anterior‐to‐middle midgut. BmGr4 was expressed in some Tk‐producing EECs in the anterior midgut, where food and its digestive products arrived 5 min after feeding began. Enzyme‐linked immunosorbent assay (ELISA) revealed secretion of Tk starting approximately 5 min after feeding began, suggesting that food sensing by BmGr4 may regulate Tk secretion. BmGr6 was expressed in a few Tk‐producing EECs in the middle‐to‐posterior midgut, although its significance was unclear. BmGr6 was also expressed in many myosuppressin‐producing EECs in the middle midgut, where food and its digestive products arrived 60 min after feeding began. ELISA revealed secretion of myosuppressin starting approximately 60 min after feeding began, suggesting that food sensing by BmGr6 may regulate myosuppressin secretion. Finally, BmGr9 was expressed in many BmK5‐producing EECs throughout the midgut, suggesting that BmGr9 may function as a sensor for the secretion of BmK5.

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A Molecular and Cellular Context-Dependent Role for Ir76b in Detection of Amino Acid Taste

Cited by 156 publications

References 44 publications

Molecular and Cellular Organization of Taste Neurons in Adult Drosophila Pharynx

Molecular and Cellular Organization of Taste Neurons in Adult Drosophila Pharynx

Physiological responses of theDrosophilalabellum to amino acids

The roles of enteroendocrine cell distribution and gustatory receptor expression in regulating peptide hormone secretion in the midgut of Bombyx mori larvae

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