The roles of enteroendocrine cell distribution and gustatory receptor expression in regulating peptide hormone secretion in the midgut of Bombyx mori larvae

Abstract

To regulate physiological homeostasis and behavior in Bombyx mori, more than 20 peptide hormones in the midgut of larvae are secreted upon detection of food substances at the lumen. Although it is logical to assume that the timings of peptide hormone secretions are regulated, little is known about the mechanisms. In this study, the distributions of enteroendocrine cells (EECs) producing five peptide hormones and EECs expressing gustatory receptors (Grs), as candidate receptors for luminal food substances and nutrients, were examined via immunostaining in B. mori larvae. Three patterns of peptide hormone distribution were observed. Tachykinin (Tk)‐ and K5‐producing EECs were located throughout the midgut; myosuppressin‐producing EECs were located in the middle‐to‐posterior midgut; and allatostatin C‐ and CCHamide‐2‐producing EECs were located in the anterior‐to‐middle midgut. BmGr4 was expressed in some Tk‐producing EECs in the anterior midgut, where food and its digestive products arrived 5 min after feeding began. Enzyme‐linked immunosorbent assay (ELISA) revealed secretion of Tk starting approximately 5 min after feeding began, suggesting that food sensing by BmGr4 may regulate Tk secretion. BmGr6 was expressed in a few Tk‐producing EECs in the middle‐to‐posterior midgut, although its significance was unclear. BmGr6 was also expressed in many myosuppressin‐producing EECs in the middle midgut, where food and its digestive products arrived 60 min after feeding began. ELISA revealed secretion of myosuppressin starting approximately 60 min after feeding began, suggesting that food sensing by BmGr6 may regulate myosuppressin secretion. Finally, BmGr9 was expressed in many BmK5‐producing EECs throughout the midgut, suggesting that BmGr9 may function as a sensor for the secretion of BmK5.

Insights into midgut cell types and their crucial role in antiviral immunity in the lepidopteran model Bombyx mori

“…In different insects, the peptides produced after cleavage are mainly involved in the regulation of food in the alimentary canal, from reduced ion transport to suppressed gut contractions ( 72 , 73 ). In B. mori tachykinin peptides (Tk) secretions are regulated by B. mori gustatory receptor ( BmGr4 ) expressed in some Tk-producing EEs in the anterior midgut when food and digestive products arrived after feeding began ( 60 ). The regulatory role of peptides secreted by EEs is evident in locusts and Drosophila .…”