“…It complements computational modeling and holds the potential to elucidate the functional mechanism of neural systems at both network and cellular levels, since it allows neuroscientists to custom-design neural circuits biologically [ 3 , 4 , 5 , 6 , 7 ]. Neuronal networks mimicking brain circuits can be built because dissociated primary neurons can form functional synapses even on non-physiological surfaces [ 8 , 9 , 10 , 11 , 12 , 13 , 14 ]. In the past, 2D-layered neuron networks were predominantly fabricated because of the advantages of easy operation and observation; however, 2D models may be confounded by atypical cell–cell or cell–matrix interactions and cellular morphology.…”