A modified single solution method for the determination of phosphate in natural waters

Murphy, Jennifer E.; Riley, J.P.

doi:10.1016/s0003-2670(00)88444-5

Cited by 18,100 publications

(9,582 citation statements)

References 6 publications

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“…Then, 20 mL of 3. 5 M HCl was added, and these samples were shaking at 22°C for 16 h. After centrifugation, concentrations of TP in the supernatant were measured by the methods in literature (Murphy and Riley 1962). To determine concentrations of P i and P o , 0.2 g of powdered samples were extracted with 20 mL 1 M HCl, for 16 h on an end-over-end shaker, then centrifuged for 20 min.…”

Section: Aquatic Macrophytes and Algae Materials Collectionmentioning

confidence: 99%

Characterization of phosphorus forms in lake macrophytes and algae by solution 31P nuclear magnetic resonance spectroscopy

Feng

Zhu

et al. 2015

Environ Sci Pollut Res

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Debris from aquatic macrophytes and algae are important recycling sources of phosphorus (P), which can result in continuing blooms of algae by recycling bioavailable P in the eutrophic lakes. However, knowledge of forms of P in aquatic macrophytes and algae and their contribution to internal loads of P in lakes is limited. Without such knowledge, it is difficult to develop appropriate strategies to remediate and or restore aquatic ecosystems that have become eutrophic. Therefore, in this work, P was extracted from six types of aquatic macrophytes and algae collected from Tai Lake of China and characterized by use of solution 31 P-nuclear magnetic resonance (NMR) spectroscopy. When extracted by 0.5 M NaOH-25 mM EDTA, extraction recovery of total P(TP) and organic P(P o ) exceeded 90 %. Concentrations of P o in algae and aquatic macrophytes were 5552 mg kg −1 and 1005 mg kg −1 and accounted for 56.0 and 47.2 % of TP, respectively. When P o , including condensed P, was characterized by solution 31 P-NMR P o in algae included orthophosphate monoesters (79.8 %), pyrophosphate (18.2 %), and orthophosphate diester (2.0 %), and P o in aquatic macrophytes included orthophosphate monoesters (90.3 %), pyrophosphate (4.2 %), and orthophosphate diester (5.5 %). Additionally, orthophosphate monoesters in algal debris mainly included β-glycerophosphate (44.1 %), α-glycerophosphate (13.5 %), and glucose 6-phosphate (13.5 %). Orthophosphate monoesters in aquatic macrophytes mainly included β-glycerophosphate (27.9 %), α-glycerophosphate (24.6 %), and adenosine 5′ monophosphate (8.2 %). Results derived from this study will be useful in better understanding nutrient cycling, relevant eutrophication processes, and pollution control for freshwater lakes.

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Section: Aquatic Macrophytes and Algae Materials Collectionmentioning

confidence: 99%

Characterization of phosphorus forms in lake macrophytes and algae by solution 31P nuclear magnetic resonance spectroscopy

Feng

Zhu

et al. 2015

Environ Sci Pollut Res

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“…Ammonium (NH 4 + ) concentration was measured with the indophenol-blue method (Mackereth et al 1989), nitrate (NO 3 -) analysis was performed with the Cd-reduction method (Elliott and Porter 1971), while the amount of urea was determined as described by Newell et al (1967). The amount of total phosphorus (TP) was measured according to Gales et al (1966) Murphy and Riley (1962).…”

Section: Laboratory Measurementsmentioning

confidence: 99%

Unique picoeukaryotic algal community under multiple environmental stress conditions in a shallow, alkaline pan

et al. 2013

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Winter phytoplankton communities in the shallow alkaline pans of Hungary are frequently dominated by picoeukaryotes, sometimes in particularly high abundance. In winter 2012 the ice-covered alkaline Zab-szék pan was found to be extraordinarily rich in picoeukaryotic green algae (42-82 × 10 6 cells ml -1 ) despite the simultaneous presence of multiple stressors (low temperature and light intensity with high pH and salinity). The maximum photosynthetic rate of the picoeukaryote community was 1.4 µg C µg chlorophyll a -1 h -1 at 125 µmol m -2 s -1 .The assimilation rates compared with the available light intensity measured on the field show that the community was considerably light-limited. Estimated areal primary production was 2 180 mg C m -2 d -1 . On the basis of the 18S rRNA gene analysis (cloning and DGGE), the community was phylogenetically heterogeneous with several previously undescribed chlorophyte lineages, which indicates the ability of picoeukaryotic communities to maintain high genetic diversity under extreme conditions.

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“…The tailings had a pH value of 8.68 (1:2.5 tailings to water), 1.63% organic matter, 0.031% total N. Total N content and organic matter were determined by the Kjeldahl method (Kjeldahl, 1883) and modified Tiurin's method (Mebius, 1960) respectively. The tailings were in short supply of available P with a content of 1.56 mg kg À1 that extracted by 0.5 mol L À1 NaHCO 3 , and determined colorimetrically by the vanadomolybdate method following the methods described by Olsen et al (1954) and Murphy and Riley (1962). The tailings contained 232 mg Cu kg, 81 mg Pb kg À1 , 54 mg As kg À1 , 2.5 mg Cd kg À1 and 189 mg Zn kg À1 .…”

Section: Mine Tailings Samplesmentioning

confidence: 99%

Effects of the arbuscular mycorrhizal fungus Glomus mosseae on growth and metal uptake by four plant species in copper mine tailings

Chen

Zhu

Duan

et al. 2007

Environmental Pollution

173

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A modified single solution method for the determination of phosphate in natural waters

Cited by 18,100 publications

References 6 publications

Characterization of phosphorus forms in lake macrophytes and algae by solution 31P nuclear magnetic resonance spectroscopy

Characterization of phosphorus forms in lake macrophytes and algae by solution 31P nuclear magnetic resonance spectroscopy

Unique picoeukaryotic algal community under multiple environmental stress conditions in a shallow, alkaline pan

Effects of the arbuscular mycorrhizal fungus Glomus mosseae on growth and metal uptake by four plant species in copper mine tailings

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