A modified method for the turbidimetric assay of nisin

Flôres, Simone Hickmann; Braga, Ana Luíza de Mattos; Alegre, Ranulfo Monte

doi:10.1590/s1516-89132003000300020

Cited by 8 publications

(2 citation statements)

References 7 publications

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“…The supernatant was carried on corn starch at 1:9 W/W and then dried at 40°C for 24 h; therefore, each 1 g of BLS contained 100 mg bacteriocin and 900 mg corn starch as a carrier. The bioassay of the produced BLS was conducted as described by Flôres et al (2003). Briefly, one tube of Lactococcus lactis subsp.…”

Section: Methodsmentioning

confidence: 99%

A newly developed bacteriocin like substance to replace monensin in diets of lactating ewes

Azzaz

Kholif

Tawab

et al. 2023

Animal Science Journal

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The aim of the present experiment was to evaluate the effect of feeding a newly produced bacteriocin‐like substance (BLS) as a replacement for monensin in the diets of lactating ewes. In Experiment 1, the effects of BLS or monensin at 0.5, 1, 1.5, and 2 g/kg diet on in vitro ruminal fermentation were compared. In Experiment 2, 30 multiparous Barki ewes were divided into three treatments in a complete randomized design for 90 days. The ewes were fed a basal diet without supplementation or supplemented with monensin or BLS at 0.5 g/kg DM diet. In Experiment 1, the highest levels of BLS and monensin decreased gas production, while all levels of additives linearly decreased methane production. In Experiment 2, BLS increased nutrient digestibility. Additives increased ruminal total and individual volatile fatty acids and decreased ruminal ammonia‐N. The BLS increased serum albumin and decreased the concentrations of serum liver enzymes, while both additives increased serum glucose and decreased urea‐N. Additives increased daily production of milk, while the BLS treatment increased the feed efficiency. It is concluded that dietary inclusion of BLS at 0.5 g/kg DM for lactating ewes is recommended to replace monensin.

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Section: Methodsmentioning

confidence: 99%

A newly developed bacteriocin like substance to replace monensin in diets of lactating ewes

Azzaz

Kholif

Tawab

et al. 2023

Animal Science Journal

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“…Further attempts are made to develop a quantitative method using automated turbidometry to assess the antimicrobial efficacy of bacteriocin-like inhibitors produced by pseudomonas aeruginosa, pediococus dammosus and pediococcus pentosaccus and the growth of the test strain namely pseudomonas aeruginosa was kinetically monitored and various growth curve parameters were used as quantitative indicators of inhibition 14 . Flores 15 , et al reported a modified turbidimetric assay method for nisin in total incubation period of approximately 6 h. using Lactococcus lactis subsp. cremoris as test microorganism.…”

Section: Introductionmentioning

confidence: 99%

Turbidimetric Assay of Nisin in Tender Coconut Water

Chauhan¹,

Kumar

Nadanasabapathi

2017

Def. Life. Sc. Jl.

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Nisin activity in tender coconut water using Lactococcus lactis subspecies cremoris as test microorganism was estimated using turbidimetry by measuring optical density at 600 nm after 6 h. of incubation at 37 °C. The test microorganism was cultivated in nutrient broth with different concentration of nisin along with tender coconut water. A standard curve was drawn between optical density and known concentration of nisin. The nisin concentration of the test samples was measured by curve fitting method using the optical density values. The results thus obtained by this method were reproducible, rapid and more accurate in tender coconut water and hence it may be employed for the determination of nisin activity in tender coconut water on regular basis for quality control purposes.

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Modification of the data-processing method for the turbidimetric bioassay of nisin

2007

Appl Microbiol Biotechnol

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The data processing method of the turbidimetric bioassay of nisin was modified to facilitate its industrial application. The influence of the initial indicator concentration was minimized by a redefined specific dose of the bacteriocin as the quotient between the titer of the added bacteriocin and the initial population density of the indicator in the suspension. It was found that dc = 0.125 microg ml(-1) was the critical dose of nisin that can cause a complete inhibition of the indicator, Pediococcus acidilactici UL5, with an initial OD of 0.135. To eliminate the interference of the cell debris, an equation, epsilonI = ODi(1-di)/OD0 = epsilonA(1-di/dc), exploiting dc, was formulated to obtain the intrinsic survival proportion. The use of the specific dose of the bacteriocin and the intrinsic survival proportion as parameters of the dose/response curve greatly enhanced its repeatability and feasibility. A dual-dosage approach was developed to further simplify the conventional standard dose/response curve method.

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A modified method for the turbidimetric assay of nisin

Cited by 8 publications

References 7 publications

A newly developed bacteriocin like substance to replace monensin in diets of lactating ewes

A newly developed bacteriocin like substance to replace monensin in diets of lactating ewes

Turbidimetric Assay of Nisin in Tender Coconut Water

Modification of the data-processing method for the turbidimetric bioassay of nisin

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