A modified low-cost colorimetric method for paracetamol (acetaminophen) measurement in plasma

Abstract

Background-Despite a significant increase in the number of patients with paracetamol poisoning in the developing world, plasma paracetamol assays are not widely available. The purpose of this study was to assess a low cost modified colorimetric paracetamol assay that has the potential to be performed in small laboratories with restricted resources.

Paracetamol overdose: Relevance of recent evidence for managing patients in Sri Lanka

“…This approach is consistent with international guidelines which use the ingested dose when paracetamol concentration is not available, easily interpreted or appropriate. However using the commonly recommended ingested dose threshold of 200 mg/kg would result in 41% of patients receiving antidote unnecessarily [23]. In this situation there is a strong case for providing effective lower risk and cheaper treatment.…”

“…Although a low cost paracetamol assay has been developed and validated in Sri Lanka, it is not available in routine patient care [23]. Thus treatment decisions are normally based upon the history of ingested dose [7,24,25].…”

A simple synthesis of nitrogen doped porous graphitic carbon: Electrochemical determination of paracetamol in presence of ascorbic acid and p-aminophenol

“…Several analytical techniques for above mentioned analysis have been developed using liquid M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 3 chromatography [15], spectrophotometry [16], chemiluminasence, flow injection methods [17], capillary electrophoresis [18], colorimetry [19], FTIR-Raman spectrometry [20]. But these methods are often expensive and time consuming with low sensitivity and selectivity.…”

Validation Thin Layer Chromatography for the Determination of Acetaminophen in Tablets and Comparison with a Pharmacopeial Method

“…Acetaminophen was quantitatively determined in different biological fluids, namely, plasma [1–6], urine [7–12], serum [11, 13], and tissue [14]. Moreover, acetaminophen was also determined in different pharmaceutical preparations in single and in combined dosage forms [15–30].…”

“…As was reported in the literature, several methods like liquid chromatography [1, 4, 5, 21], high performance liquid chromatography [3, 8, 10, 12, 17, 18], reversed-phase sequential injection chromatography (SIC) [20], spectrophotometric [7, 9, 13, 16], spectrophotometric with spectrodensitometric [22], spectrofluorimetric [15], capillary electrophoresis [11, 19], colorimetric [2, 6], chronoamperometric [14], thin layer chromatography with spectrophotometric [23], and thin layer chromatography with a fluorescence plate reader [24], and thin layer chromatography (HPTLC) with a densitometry were successfully applied in qualitative and quantitative acetaminophen analysis [25–30]. Generally, HPLC and UV-spectrophotometric methods have been reported in the United States and Polish Pharmacopeias for the analysis of acetaminophen in pharmaceutical preparations [31, 32].…”