Validation Thin Layer Chromatography for the Determination of Acetaminophen in Tablets and Comparison with a Pharmacopeial Method

Pyka, Alina; Budzisz, Marika; Dołowy, Małgorzata

doi:10.1155/2013/545703

Cited by 19 publications

(14 citation statements)

References 32 publications

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“…The nonsteroidal analgesic PA is extremely safe and highly effective 31. There have been several reports on the determination of Paracetamol using various techniques, including high performance liquid chromatography 32, reverse phase performance liquid chromatography 33, thin layer chromatography 34, gas chromatography 35, 36, UV‐spectrophotometric method 37, spectrophotometric flow injection 38 respectively. Nevertheless, they are of high costs and time‐consuming.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Preparation of Yttrium Hexacyanoferrate on Reduced Graphene Oxide and Its Application to Analgesic Drug Sensor

et al. 2014

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Herein, we report a template free and surfactant less electrochemical approach for the preparation of flower‐like yttrium hexacyanoferrate (YHCF) particles on reduced graphene oxide (RGO) modified glassy carbon electrode (GCE). The morphology of YHCF particles has been controlled by varying the molar ratio of Y(NO3)2 and K3Fe(CN)6 for the first time. The surface morphology of as‐prepared RGO/YHCF composite was characterized using SEM, EDX, IR and XRD methods. The electrocatalytic activity of the RGO/YHCF composite modified GCE towards Paracetamol (PA) oxidation has been investigated by using cyclic voltammetry (CV) and linear sweep voltammetry (LSV). Besides, the practical feasibility of the fabricated modified GCE has been demonstrated through the determination of PA from commercially purchased Paracetamol tablets.

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Section: Introductionmentioning

confidence: 99%

Electrochemical Preparation of Yttrium Hexacyanoferrate on Reduced Graphene Oxide and Its Application to Analgesic Drug Sensor

et al. 2014

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“…The levels of factors investigated and the experimental design matrix (2 3 ) are shown in Table 1 . A high level is represented by “+” and a low level by “−.” The ways of calculation of the effects ( E ) characterizing the particular individual factors and rank probabilities were earlier presented [ 18 – 23 ].…”

Section: Methodsmentioning

confidence: 99%

Validation of a Thin-Layer Chromatography for the Determination of Hydrocortisone Acetate and Lidocaine in a Pharmaceutical Preparation

Dołowy

Kulpińska-Kucia

Pyka

2014

The Scientific World Journal

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A new specific, precise, accurate, and robust TLC-densitometry has been developed for the simultaneous determination of hydrocortisone acetate and lidocaine hydrochloride in combined pharmaceutical formulation. The chromatographic analysis was carried out using a mobile phase consisting of chloroform + acetone + ammonia (25%) in volume composition 8 : 2 : 0.1 and silica gel 60F254 plates. Densitometric detection was performed in UV at wavelengths 200 nm and 250 nm, respectively, for lidocaine hydrochloride and hydrocortisone acetate. The validation of the proposed method was performed in terms of specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, and robustness. The applied TLC procedure is linear in hydrocortisone acetate concentration range of 3.75 ÷ 12.50 μg·spot−1, and from 1.00 ÷ 2.50 μg·spot−1 for lidocaine hydrochloride. The developed method was found to be accurate (the value of the coefficient of variation CV [%] is less than 3%), precise (CV [%] is less than 2%), specific, and robust. LOQ of hydrocortisone acetate is 0.198 μg·spot−1 and LOD is 0.066 μg·spot−1. LOQ and LOD values for lidocaine hydrochloride are 0.270 and 0.090 μg·spot−1, respectively. The assay value of both bioactive substances is consistent with the limits recommended by Pharmacopoeia.

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“…Thus, in this study, thin-layer chromatography was used to test for the separation performance of alternative solvents with the presence of analgesics (i.e., acetaminophen) in a commercial product setting. Other studies have used caffeine for the same reason as well [33,34].…”

Section: Addition Of Caffeine As a Second Analytementioning

confidence: 99%

“…Caffeine was added to the previously prepared acetaminophen solution to compare the abilities of the solvents to separate the two different components within a mixture. Caffeine was used as a stimulant for API (i.e., acetaminophen in this case), while acetaminophen served as the model API for evaluating the separation efficiency of safer solvents [33,34]. Notably, caffeine is the most widely used stimulant because it has fatigue-reducing properties [35].…”

Section: Addition Of Caffeine As a Second Analytementioning

confidence: 99%

Designing Safer Solvents to Replace Methylene Chloride for Liquid Chromatography Applications Using Thin-Layer Chromatography as a Screening Tool

Sharma

Morose

et al. 2021

Separations

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Methylene chloride, commonly known as dichloromethane (DCM), is a widely used chemical for chromatography separation within the polymer, chemical, and pharmaceutical industries. With the ability to effectively solvate heterocyclic compounds, and properties including a low boiling point, high density, and low cost, DCM has become the solvent of choice for many different applications. However, DCM has high neurotoxicity and is carcinogenic, with exposure linked to damage to the brain and the central nervous system, even at low exposure levels. This research focuses on sustainability and works towards finding safer alternative solvents to replace DCM in pharmaceutical manufacturing. The research was conducted with three active pharmaceutical ingredients (API) widely used in the pharmaceutical industry: acetaminophen, aspirin, and ibuprofen. Thin-layer chromatography (TLC) was used to investigate if an alternative solvent or solvent blend could show comparable separation performance to DCM. The use of the Hansen Solubility Parameter (HSP) theory and solubility testing allowed for the identification of potential alternative solvents or solvent blends to replace DCM. HSP values for the three APIs were experimentally determined and used to identify safer solvents and blends that could potentially replace DCM. Safer solvents or binary solvent blends were down-selected based on their dissolution power, safety, and price. The down-selected solvents (e.g., ethyl acetate) and solvent blends were further evaluated using three chemical hazard classification approaches to find the best fitting nonhazardous replacement to DCM. Several safer solvent blends (e.g., mixtures composed of methyl acetate and ethyl acetate) with adequate TLC performance were identified. Results from this study are expected to provide guidance for identifying and evaluating safer solvents to separate APIs using chromatography.

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Validation Thin Layer Chromatography for the Determination of Acetaminophen in Tablets and Comparison with a Pharmacopeial Method

Cited by 19 publications

References 32 publications

Electrochemical Preparation of Yttrium Hexacyanoferrate on Reduced Graphene Oxide and Its Application to Analgesic Drug Sensor

Electrochemical Preparation of Yttrium Hexacyanoferrate on Reduced Graphene Oxide and Its Application to Analgesic Drug Sensor

Validation of a Thin-Layer Chromatography for the Determination of Hydrocortisone Acetate and Lidocaine in a Pharmaceutical Preparation

Designing Safer Solvents to Replace Methylene Chloride for Liquid Chromatography Applications Using Thin-Layer Chromatography as a Screening Tool

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