A modified, hypoallergenic variant of the <i>Ricinus communis</i> Ric c1 protein retains biological activity

Pacheco-Soares, Thaís; Carvalho, André de Oliveira; Araújo, Jucélia da Silva; Souza, Giliane da Silva de; Machado, Olga Lima Tavares

doi:10.1042/bsr20171245

Cited by 5 publications

(6 citation statements)

References 25 publications

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“…Furthermore, the presence of 2S albumins, reserve proteins, and allergens in R. communis seeds may also be related to the mortality of M. persicae, as they act as inhibitors of the activity of the α-amylase enzyme of the Zabrotes subfasciatus Boheman (Coleoptera: Bruchidae), Callosobruchus maculatus Fab. (Coleoptera: Bruchidae) and Tenebrio molitor L. (Coleoptera: Tenebrionidae) insects [22]. Thus, probably, the substances existing in the different oils simultaneously affect the effect of mortality when they are in balance.…”

Section: Resultsmentioning

confidence: 99%

Interaction between Castor Bean oil and Jatropha oil to Control the Brassica Aphids

Piffer

Holtz

Botti

et al. 2023

IJPSS

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The aim of this work was to study the effect of the interaction between castor bean oil (Ricinus communis) and jatropha oil (Jatropha curcas) on the aphid, Myzus persicae. The experiment was realized at the Institute Federal of Espírito Santo in Brazil. Tests were conducted in a completely randomized design, with 11 treatments and 10 repetitions, arranged in a factorial arrangement of 11 (interactions between oils) x 2 (application forms). The concentration used in this test was 3 %. Treatment interaction ratios were 0-100; 10-90; 20-80; 30 -70; 40-60; 50-50; 60-40; 70-30; 80-20; 90-10; 100-0 % jatropha and castor bean oil, respectively. Tests of direct and indirect application were performed. The evaluations were performed 24, 48, and 72 hours after the applications. For both forms of application, there were significant mortalities. Mortalities greater than 70 % were observed when the interaction contained more significant amounts of jatropha than castor bean. However, a negative interaction was observed between concentrations with similar proportions. In conclusion, for both application forms, the interaction between jatropha and castor bean oils effectively reduce the population of M. persicae.

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Section: Resultsmentioning

confidence: 99%

Interaction between Castor Bean oil and Jatropha oil to Control the Brassica Aphids

Piffer

Holtz

Botti

et al. 2023

IJPSS

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“…Competent cells of these strains were chemically prepared, as previously reported (Pacheco-Soares and Carvalho, 2018).…”

Section: Biological Materials and Animalsmentioning

confidence: 99%

“…Anti-2S albumin serum: An anti-2S albumin from castor seeds was generated in rabbits as described (Deus-de-Oliveira et al, 2011) 2.3. Natural Ric c3 (nRic c3) isolation and insertion in a cloning vector nRic c3 isolation: The 2S albumin pool (Ric c1 and Ric c3) was isolated and characterized by SDS/PAGE and immunoblotting as previously described (Pacheco-Soares and Carvalho, 2018), and the isoforms Ric c1 and Ric c3 were isolated as described by Felix et al (2008).…”

Section: Biological Materials and Animalsmentioning

confidence: 99%

“…In silico study proposed the theoretical deactivation of the allergenic epitopes of Ric c1 and Ric c3 using mutations in the glutamic acid residues (E), present in IgE -binding. Glutamic residues were exchanged for leucine residues (L), making -them the least allergenic and without significantly altering the structures of these proteins (Nascimento et al, 2011;Pacheco-Soares and Carvalho, 2018). Based on this, Pacheco-Soares and Carvalho (2018) established conditions for expressing the Brazilian Journal of Biology, 2023, vol.…”

Section: Introductionmentioning

confidence: 99%

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Production of a Ric c3 hypo-allergen with no IgE binding or anaphylactogenic activity

Bartholazzi,

Lodi,

Mello

et al. 2023

Braz. J. Biol.

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Several studies have been carried out to expand the use of Ricinus communis L. castor bean (Ricinus communis L castor bean.). This oilseed finds appropriate conditions for its development in Brazil, with more than 700 applications. The main allergens of this plant are Ric c1 and Ric c3, that cross-react with various aeroallergens and food allergens such as peanuts, soybeans, corn, and wheat. This study aimed to determine the effect of mutations in Ric c3 amino acid residues known to affect IgE binding and allergy challenges. Based on the Ric c3 structure, B-cell epitopes, and amino acid involved in IgE binding, we produce recombinant mutant protein, mrRic c3, secreted from E. coli. Strategic glutamic acid residues in IgE-biding regions were changed by Leucine. The allergenicity of mrRic c3 was evaluated by determination of IgE, IgG1, and total IgG in immunized Balb/c mice and by degranulation assays of mast cells isolated from Wistar rats. The mrRic c3 presented a percentage of mast cell degranulation close to that seen in the negative control, and the immunization of mice with mrRic c3 presented lower levels of IgE and IgG1 than the group treated with the protein without mutations. The mutant mrRic c3 had an altered structure and reduced ability to stimulate pro-inflammatory responses and bind IgE but retained its ability to induce blocking antibodies. Thus, producing a hypoallergenic mutant allergen (mrRic c3) may be essential in developing new AIT strategies.

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“…The castor bean (Ricinus communis L. (Euphorbiaceae)) has insecticidal properties in virtually all its plant parts, such as leaves, stems, roots, and seeds (RIOBA & STEVENSON 2020). For example, in the case of the seeds, they are rich in different inhibitors, which act on α-amylases and prevent starch absorption by the insect and may lead to starvation death (PACHECO-SOARES et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Management of Myzus persicae with leaves of Jatropha curcas and Ricinus communis in different vegetative stages

Holtz

Stinguel

Ataide

et al. 2022

Rev. Ciênc. Agrovet.

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Although chemical insecticides are successfully used in agriculture, several problems may be related to their indiscriminate use. This has encouraged the development of alternative methods for pest insect control and societal pressure for pesticide-free products. The jatropha (J. curcas) and castor bean (Ricinus communis) are plants that have shown insecticidal potential. Thus, the objective of this work was to study the effects of leaves of R. communis and J. curcas of different ages on the cabbage aphid (Myzus persicae). Five solution concentrations and five leaf types were used. Each treatment consisted of ten individuals M. persicae, kept in Petri dishes (10.0 x 1.2 cm), about kale leaf discs. Each Petri dish was considered a repeat, totaling ten. An airbrush connected to a compressor calibrated at constant pressure and 5 mL of solution per repetition was used for direct application. The cabbage discs were immersed in the different treatments for five seconds and offered to aphids in the indirect application. For both tests, evaluations were performed 72 hours after application. Data were submitted to linear regression analysis and means test. There was an interaction between the application type, leaf type, and concentrations for both plants. We argue that at their different ages, jatropha and castor bean leaves have insecticidal potential in the management of M. persicae.

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A modified, hypoallergenic variant of the Ricinus communis Ric c1 protein retains biological activity

Cited by 5 publications

References 25 publications

Interaction between Castor Bean oil and Jatropha oil to Control the Brassica Aphids

Interaction between Castor Bean oil and Jatropha oil to Control the Brassica Aphids

Production of a Ric c3 hypo-allergen with no IgE binding or anaphylactogenic activity

Management of Myzus persicae with leaves of Jatropha curcas and Ricinus communis in different vegetative stages

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