A Modified Colorimetric Method for the Estimation of N-Acetylamino Sugars

Reissig, José L.; Strominger, Jack L.; Leloir, Luis F.

doi:10.1016/s0021-9258(18)65959-9

Cited by 2,357 publications

(175 citation statements)

References 8 publications

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“…The activity of hyaluronidase was determined through UV spectroscopy, by measuring the amount of N-acetylglucosaminoglycan, which is formed by the degradation of hyaluronic acid [53]. An amount of 400 U/mL of Hyalurorindase from bovine testes (Type I-S, lyophilized powder, 400-1000 units/mg, Sigma, Germany) was dissolved in 0.1 M acetate buffer, pH 3.5 and incubated with the aqueous solutions of the respective ligands at concentrations of 300 µg/mL and 500 µg/mL for 20 min at 37 • C. After the incubation of the mixture, 0.5 mg/mL BSA (Bovine Serum Albumin, lyophilized powder, crystallized ≥ 98%, Sigma, Germany) was added in each sample, and then water to reach a total volume of 200 µL.…”

Section: Evaluation Of the Inhibition Of S Euboea Ethyl Acetate Residue And Its Isolated Compounds Towards Hyaluronidasementioning

confidence: 99%

Anti-Ageing Potential of S. euboea Heldr. Phenolics

et al. 2021

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In recent years, the use of Sideritis species as bioactive agents is increasing exponentially. The present study aimed to investigate the chemical constituents, as well as the anti-ageing potential of the cultivated Sideritis euboea Heldr. The chemical fingerprinting of the ethyl acetate residue of this plant was studied using 1D and 2D-NMR spectra. Isomeric compounds belonging to acylated flavone derivatives and phenylethanoid glycosides were detected in the early stage of the experimental process through 2D-NMR techniques. Overall, thirty-three known compounds were isolated and identified. Some of them are reported for the first time not only in S. euboea, but also in genus Sideritis L. The anti-ageing effect of the ethyl acetate residue and the isolated specialized products was assessed as anti-hyaluronidase activity. In silico docking simulation revealed the interactions of the isolated compounds with hyaluronidase. Furthermore, the in vitro study on the inhibition of hyaluronidase unveiled the potent inhibitory properties of ethyl acetate residue and apigenin 7-O-β-d-glucopyranoside. Though, the isomers of apigenin 7-O-p-coumaroyl-glucosides and also the 4′-methyl-hypolaetin 7-O-[6′′′-O-acetyl-β-d-allopyranosyl]-(1→2)-β-d-glucopyranoside exerted moderate hyaluronidase inhibition. This research represents the first study to report on the anti-hyaluronidase activity of Sideritis species, confirming its anti-inflammatory, cytotoxic and anti-ageing effects and its importance as an agent for cosmetic formulations as also anticancer potential.

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Section: Evaluation Of the Inhibition Of S Euboea Ethyl Acetate Residue And Its Isolated Compounds Towards Hyaluronidasementioning

confidence: 99%

Anti-Ageing Potential of S. euboea Heldr. Phenolics

et al. 2021

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“…The tubes were heated for 3 min in a boiling water bath and cooled in tap water. p-Dimethylaminobenzaldehyde reagent (1.5 ml), prepared as described by Reissig et al, 16 was added to each sample, and these were then vortexed, heated at 37°C for 20 min, briefly centrifuged and read Hyaluronic acid as a marker of liver fibrosis using a colorimeter at 585 nm. Consequently, the amount of reaction product (reducing Nacetylglucosamine termini) was determined.…”

Section: Hyaluronidase Assaymentioning

confidence: 99%

Evaluation of Serum Hyaluronic Acid Level and Hyaluronidase Activity in Acute and Chronic Hepatitis C

et al. 2007

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Following major tissue injury, hyaluronic acid production increases as a rapid response survival mechanism. Increased hyaluronic acid production and turnover are often associated with increased hyaluronidase activity, the enzyme that degrades hyaluronic acid. We investigated whether hyaluronic acid and hyaluronidase can be used as non-invasive markers of acute disease activity in hepatitis C by studying 26 patients with acute hepatitis C, 89 with chronic hepatitis C and 32 healthy controls. Chronic hepatitis C subjects were classified into five subgroups according to the stage of liver fibrosis. Serum aspartate aminotransferase and alanine aminotransferase activities and hyaluronic acid levels were increased in hepatitis C patients compared with the controls. Serum hyaluronic acid elevation correlated with disease progression. Serum hyaluronidase activities were also increased in patients compared with the controls, but decreased with disease progression. We conclude that both hyaluronidase and hyaluronic acid may be useful as early non-invasive serum indicators of disease activity in acute hepatitis C.

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“…The fluorimetric Morgan-Elson assay method was performed according to Reissig, et al [62] that modified by Takahashi, et al [63]. In a brief description, a 5 µL of tested EOs and a mixture of EOs of the two plants (1:1, w/w), separately, were incubated for 10 min at 37 • C with bovine hyaluronidase (1.50 U) in 100 µL of 20 mM sodium phosphate buffer solution (pH 7.0), sodium chloride (77 mM), in addition to 0.01% bovine serum albumin (BSA).…”

Section: Anti-hyaluronidase Assaymentioning

confidence: 99%

Chemical Profiles, Anticancer, and Anti-Aging Activities of Essential Oils of Pluchea dioscoridis (L.) DC. and Erigeron bonariensis L.

Elgamal

Ahmed²,

Abd‐ElGawad

et al. 2021

Plants

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Plants belonging to the Asteraceae family are widely used as traditional medicinal herbs around the world for the treatment of numerous diseases. In this work, the chemical profiles of essential oils (EOs) of the above-ground parts of Pluchea dioscoridis (L.) DC. and Erigeron bonariensis (L.) were studied in addition to their cytotoxic and anti-aging activities. The extracted EOs from the two plants via hydrodistillation were analyzed by gas chromatography-mass spectroscopy (GC-MS). GC-MS of EO of P. dioscoridis revealed the identification of 29 compounds representing 96.91% of the total oil. While 35 compounds were characterized from EO of E. bonariensis representing 98.21%. The terpenoids were found the main constituents of both plants with a relative concentration of 93.59% and 97.66%, respectively, including mainly sesquiterpenes (93.40% and 81.06%). α-Maaliene (18.84%), berkheyaradulen (13.99%), dehydro-cyclolongifolene oxide (10.35%), aromadendrene oxide-2 (8.81%), β-muurolene (8.09%), and α-eudesmol (6.79%), represented the preponderance compounds of EO of P. dioscoridis. While, trans-α-farnesene (25.03%), O-ocimene (12.58%), isolongifolene-5-ol (5.53%), α-maaliene (6.64%), berkheyaradulen (4.82%), and α-muurolene (3.99%), represented the major compounds EO of E. bonariensis. A comparative study of our results with the previously described data was constructed based upon principal component analysis (PCA) and agglomerative hierarchical clustering (AHC), where the results revealed a substantial variation of the present studied species than other reported ecospecies. EO of P. dioscoridis exhibited significant cytotoxicity against the two cancer cells, MCF-7 and A-549 with IC50 of 37.3 and 22.3 μM, respectively. While the EO of the E. bonariensis showed strong cytotoxicity against HepG2 with IC50 of 25.6 μM. The EOs of P. dioscoridis, E. bonariensis, and their mixture (1:1) exhibited significant inhibitory activity of the collagenase, elastase, hyaluronidase, and tyrosinase comparing with epigallocatechin gallate (EGCG) as a reference. The results of anti-aging showed that the activity of mixture (1:1) > P. dioscoridis > E. bonariensis against the four enzymes.

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A Modified Colorimetric Method for the Estimation of N-Acetylamino Sugars

Cited by 2,357 publications

References 8 publications

Anti-Ageing Potential of S. euboea Heldr. Phenolics

Anti-Ageing Potential of S. euboea Heldr. Phenolics

Evaluation of Serum Hyaluronic Acid Level and Hyaluronidase Activity in Acute and Chronic Hepatitis C

Chemical Profiles, Anticancer, and Anti-Aging Activities of Essential Oils of Pluchea dioscoridis (L.) DC. and Erigeron bonariensis L.

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