Anti-Ageing Potential of S. euboea Heldr. Phenolics

Over the last years, Sideritis extracts were shown to improve memory. However, their potential to promote the generation of new neurons, starting with the neuronal differentiation of neural stem cells, remains unexplored. Therefore, the present study aimed to evaluate the neurogenic effects of different Sideritis infusions in neural stem and precursor cells and their impacts on cell viability. Moreover, the metabolic fingerprints were recorded using LC-DAD, LC-ESIHRMS, and GC-MS. The neurogenic potential from infusions of the eight Sideritis taxa tested was as potent as the classical neuronal inducer combination of retinoic acid and valproic acid. Further cytotoxicity assays revealed that the IC50 values of extracts were comprised between 163 µg/mL and 322 µg/mL. Hierarchical cluster analyses of the metabolic fingerprints unveiled, that the two Sideritis taxa with the lowest IC50 values were the most divergent in the analytical techniques used. As the analysis focused on polyphenols, it is reasonable to assume that these compounds are responsible for the effect on the cell viability of SH-SY5Y neuroblastoma cells. This study is the first report on the neurogenic potential of Sideritis taxa and might support the use of Sideritis herbal preparations in the context of neurodegenerative diseases.

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“…4 c ). This is in accordance with previous findings in the specific plants 10 , 28 , 29 , 30 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 .…”

Section: Resultssupporting

confidence: 94%

“…4 c). This is in accordance with previous findings in the specific plants [10,[28][29][30][32][33][34][35][36][37][38][39][40].…”

Section: Resultssupporting

confidence: 94%

Metabolic Fingerprinting of Different Sideritis Taxa Infusions and Their Neurogenic Activity

et al. 2023

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“…perfoliata with those of S. cypria, we compared them with NMR spectra of previously isolated compounds from our works in Sideritis spp. [5,8,9,12,13]. Through the screening of the 1 H-NMR spectra of different compounds, we carefully noticed that the main different hydrogen signals in the spectrum of infusion of S. perfoliata subsp.…”

Section: Resultsmentioning

confidence: 99%

NMR Fingerprint Comparison of Cultivated Sideritis spp. from Cyprus

et al. 2021

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Medicinal and Aromatic Plants (MAPs) play an essential role in global health systems, since more than 80% of population use natural products in primary healthcare. Given that the global use of herbal medicines is exponentially increasing, as well as many MAPs products are introduced into the market, many cultivation practices are applied to produce high quality and standardized plant raw materials. Thus, the present study focuses on the chemical fingerprints of two cultivated Sideritis species. In Cyprus, Sideritis cypria Post and S. perfoliata L. subsp. perfoliata are widely used in traditional medicine. To date, there is no research work presenting the comparative chemical profiling between the aforesaid species using NMR methods. In this study, 1D and 2D NMR experiments were used to compare the chemical fingerprints of these species originated from conventional cultivation practices. Iridoids (ajugol, monomelittoside, and melittoside) and one flavone (4′-methyl-isoscutellarein 7-O-[6‴-O-acetyl]-β-D-allosyl(1→2)glucoside) were present in the infusion of S. perfoliata subsp. perfoliata. The phenylethanoid glycoside, acteoside, was detected in both samples. The phytochemical profiles of these cultivated species were similar to those of Sideritis species, indicating the positive impact of cultivation practices in MAPs.

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“…The management of inflammation and neuropathic pain mainly succeed by the management of pro-inflammatory mediators (NF-κB, TNF-α, IL-1β, and IL-6) [ 12 , 13 ]. Other studies have demonstrated the anti-aging effect of Sideritis extracts, by anti-hyaluronidase activity [ 32 , 33 ] and the antimicrobial activities [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

Siderol Inhibits Proliferation of Glioblastoma Cells and Acts Synergistically with Temozolomide

et al. 2022

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Glioblastoma (GBM) is the most aggressive primary central nervous system (CNS) tumor in adults with dismal prognosis. Currently, the therapeutic interventions include gross total resection, when possible, followed by radiotherapy and chemotherapy. However, despite treatment, tumor usually recurs within 7–9 months. The presence of glioma cells with stem-like properties and tumor’s heterogeneity have been identified as the most important factors driving recurrence. Recently, research efforts have been focused on the use of natural substances as treatment for GBM. Siderol is an ent-kaurane diterpenoid, isolated from the genus Sideritis. Sideritis extracts have already been investigated for their anti-inflammatory, antioxidant, and anticancer effects. In this study, we investigated the antitumoral effects of siderol in GBM T98 and U87 cell lines, as well as the effects of combined treatment with temozolomide (TMZ). Cell viability was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and trypan blue exclusion assay. Different concentrations of siderol were used in order to calculate the IC50 values at 72 h after treatment. Flow cytometry used for the DNA cell cycle analysis after treatment with siderol in concentrations of IC50 and twice the IC50 values for 72 h. Furthermore, the effect of siderol in cell’s migratory ability was tested using wound healing assay. Cell viability and proliferation, after combined treatment with siderol and TMZ, also were evaluated with the trypan blue exclusion assay and the effects of the combination treatment were analyzed with CompuSyn software. Treatment with siderol significantly reduced cell viability in T98 and U87 cell lines in a dose-dependent manner and IC50 values were calculated, 18 μM and 13 μM, respectively. Moreover, siderol induced G0/G1 cell cycle arrest in a dose-dependent manner and inhibited the migration in both cell lines. In addition, siderol and TMZ seem to have synergistic action in the majority of tested concentrations in both T98 and U87 cells. In conclusion, siderol may represent an innovative strategy for the treatment of GBM, and further studies are needed on siderol’s efficacy and mode of action.

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Anti-Ageing Potential of S. euboea Heldr. Phenolics

Cited by 9 publications

References 60 publications

Metabolic Fingerprinting of Different Sideritis Taxa Infusions and Their Neurogenic Activity

Metabolic Fingerprinting of Different Sideritis Taxa Infusions and Their Neurogenic Activity

NMR Fingerprint Comparison of Cultivated Sideritis spp. from Cyprus

Siderol Inhibits Proliferation of Glioblastoma Cells and Acts Synergistically with Temozolomide

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