A modified chemiluminescence method for hydrogen peroxide determination in apple fruit tissues

Lu, Shengmin; Song, Jun; Campbell-Palmer, Leslie

doi:10.1016/j.scienta.2008.11.003

Cited by 35 publications

(21 citation statements)

References 31 publications

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“…(Bellincampi et al 2000). Fresh leaves (4 g) were homogenized with 8 mL of 100 mM sodium phosphate buffer (pH 7.2), and 10% (W/V) polyvinylpolypyrrolidone (PVPP) (Lu et al 2009). The homogenates were centrifuged at 10,000g for 10 min at 4°C.…”

Section: Growth and Chlorophyll Content Assaysmentioning

confidence: 99%

Effects of nitrogen on the activity of antioxidant enzymes and gene expression in leaves of Populus plants subjected to cadmium stress

Zhang

Wan

Zheng

et al. 2014

Journal of Plant Interactions

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The research aimed to verify the important physiological effect of nitrogen (N) on plants exposed to cadmium (Cd). The poplar plants were grown in a Hoagland nutrient solution and treated with extra N, Cd, and N + Cd. After treatment, plant growth and chlorophyll content were recorded. The oxidative stress, the activity of antioxidant enzymes, and the expression of related genes were also examined. The results indicated the plants treated with sole Cd presented obvious toxicity symptoms, i.e. growth inhibition, reactive oxygen species accumulation, and chlorophyll content decrement. However, when N was added to the plants under Cd stress, plant growth was enhanced, chlorophyll synthesis was promoted, and the oxidative stress was alleviated. Further, the expression of antioxidant enzymes genes was upregulated by N. The results indicated that N partially reversed the toxic effect of Cd on poplar plants, which can provide new methodology to enhance the phytoremediation technology for heavy metal pollution soil.

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Section: Growth and Chlorophyll Content Assaysmentioning

confidence: 99%

Effects of nitrogen on the activity of antioxidant enzymes and gene expression in leaves of Populus plants subjected to cadmium stress

Zhang

Wan

Zheng

et al. 2014

Journal of Plant Interactions

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“…The result of this study showed the similar results with a study conducted by Reksodiputro et al in 2004 andSchenk in 2007, suggested the effectivity of the tooth bleaching by strawberry juice due to the presence of hydrogen peroxide and ellagic acid in strawberries. 7,8 Group II (immersed in apple juice three times a day) showed a significant difference in the teeth whitening degree compared to group III (immersed in mineral water three times a day). The result of this study showed the similar results with a study conducted by Schenk in 2007 and Aulia et al in 2009, suggested the effectivity of the tooth bleaching by apple juice due to the presence of hydrogen peroxide and ellagic acid in apples.…”

Section: Discussionmentioning

confidence: 95%

“…Many similar studies have been developed recently in the search for alternative natural teeth whitening compounds relatively safe (minimum side effect) for the oral cavity tissues. 4,5,7 Schenk at the California State Science Fair in 2007 described several types of fruits and vegetables with tooth whitening potentials, which were carrots, oranges, pears, apples, strawberries, and lemons. Strawberry and apple contain ellagic acid (C 14 H 6 O 8 ).8 Ellagic acid has potentials OH group as a strong oxidizing agent, as well as hydrogen peroxide.…”

Section: 45mentioning

confidence: 99%

Differences in the tooth whitening effect between strawberry juice and apple juice in-vitro

2012

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Bleaching is the tooth whitening by applying chemical materials oxidizing the organic tooth pigmentation and creating smaller and lighter molecules. Commonly used in the tooth bleaching is hydrogen peroxide. Strawberry and apple contain hydrogen peroxide and ellagic acid which will bond with an unsaturated bond of the tooth pigmentation. The purpose of this research was to finding out and measuring the tooth whitening level and effectivity between the strawberry and apple juice. The type of this research was a true experimental (in-vitro), using 30 samples of maxillary premolars with cutted radicular until the CEJ. Samples were divided into 3 groups (immersed in strawberry juice; apple juice; and mineral water); with three times daily immersion in one week. The tooth colour level was measured using a spectrophotometer. Data were analyzed using the one-way ANOVA and LSD test. The results showed significant differences among all groups. Normality test showed the variance between homogenous groups, with the p-value of 0.198 (p ≥ 0.05). The one-way ANOVA test results showed a significance value (0.000), indicated a significant degree of the tooth whitening level between all groups. The LSD test results showed that the tooth whitening level in group I (immersed in strawberry juice) was significantly different to group II (immersed in apple juice) and group III (immersed in mineral water), with a significance value of 0.01 and 0.00 ( p ≤ 0.05). The tooth whitening level in group II was significantly different from group III, with a significance value of 0.03 (p ≤ 0.05). There were differences in the tooth whitening level between immersion in strawberry juice, apple juice, and mineral water, with the most effective tooth whitening level found in the strawberry juice immersion.

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“…The quantitative determination of hydrogen peroxide is important in numerous studies since H 2 O 2 is involved in oxidative cellular damages as well as in signalling processes [2] [3]. Owing to its importance, numerous methods and kits have been developed based on spectrophotometry, chemiluminescence [4], enzymatic method [5] and electrochemistry [6]. However, some of these methods are very expensive.…”

Section: Introductionmentioning

confidence: 99%

Optimized Assay for Hydrogen Peroxide Determination in Plant Tissue Using Potassium Iodide

Junglee¹,

Urban²,

Sallanon³

et al. 2014

AJAC

318

137

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Here, we present an optimization of colorimetric determination of hydrogen peroxide content in plants using potassium iodide. Our method is based on a one step buffer (extraction and reaction) for the determination of H 2 O 2 in different plant tissues and overcomes interference of soluble antioxidant and color background. A particular attention is paid to buffer pH shown to be tissue dependent. With this inexpensive microplate method, it is possible to analyze 12 experimental samples in about 45 min all in triplicates, with blanks, controls and standard curve.

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A modified chemiluminescence method for hydrogen peroxide determination in apple fruit tissues

Cited by 35 publications

References 31 publications

Effects of nitrogen on the activity of antioxidant enzymes and gene expression in leaves of Populus plants subjected to cadmium stress

Effects of nitrogen on the activity of antioxidant enzymes and gene expression in leaves of Populus plants subjected to cadmium stress

Differences in the tooth whitening effect between strawberry juice and apple juice in-vitro

Optimized Assay for Hydrogen Peroxide Determination in Plant Tissue Using Potassium Iodide

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