A modification of the cell culture agar diffusion test using fluoresceindiacetate staining

Schmalz, Gottfried; Netuschil, L

doi:10.1002/jbm.820190605

Cited by 23 publications

(7 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, this method is considered to be only qualitative, or at most, semi-quantitative in nature. Furthermore, some dental filling materials contain or produce considerable amounts of ingredients, which if applied to cells in culture; the morphology of the cells will appear to be normal, indicating no cell damage even though the cells are no longer vital [38]. The use of membrane effects, cell activity and proliferation rate have no such drawbacks.…”

Section: Means Of Cytotoxicity Testingmentioning

confidence: 97%

Biocompatibility of dental alloys used in dental fixed prosthodontics

Elshahawy

Watanabe

2014

Tanta Dental Journal

View full text Add to dashboard Cite

Many different types of alloys are now available in the market to be used for dental restorations and fixed prostheses. The common criterion for all these fixed prosthodontic materials is the permanent existence of them in the oral cavity for prolonged time without the ability to be removed by the patient. Therefore, knowledge about the biocompatibility of dental alloys is of great importance. This article presents a literature review on the biocompatibility of dental alloys. A PubMed database search was conducted for studies pertaining to the biocompatibility of dental alloys. The search was limited to peer-reviewed articles published in English between 1985 and 2013. Available data revealed that substances are released from alloys into the surrounding tissues; mainly nickel, zinc, and copper. Some alloys such as nickelechromium alloy have shown to be cytotoxic in vitro. Also, elements released from gold alloy showed in vitro cytotoxic effect. Therefore, clinicians should give up assuming that gold alloy is completely inert and biocompatible with oral tissues. The clinical relevance of these findings remains unclear. Further in vitro studies, as well as controlled clinical trials, are needed due to possible exceptions.

show abstract

Section: Means Of Cytotoxicity Testingmentioning

confidence: 97%

Biocompatibility of dental alloys used in dental fixed prosthodontics

Elshahawy

Watanabe

2014

Tanta Dental Journal

View full text Add to dashboard Cite

show abstract

“…The second involved a more basic change (series 3): instead of using SDHactivity as a marker for cell vita!ity, fluorescein diacetate (FDA) was used for that purpose. FDA was introduced in the in vitro cytotoxicity testing of dental materials [18][19][20][21]. Through the transformation of non-fluorescent FDA into flourescing fluorescein by non-specific hydrolases, metabolic activity of a cell can easily be visualized [19].…”

confidence: 99%

New developments in the filter test system for cytotoxicity testing

Schmalz

Hiller

Dörter-Aslan

1994

J Mater Sci: Mater Med

Self Cite

View full text Add to dashboard Cite

The objective of this study was (1) to improve the succinate dehydrogenase (SDH) staining procedure of the filter test system,.and (2) to study the suitability of hydrolases as markers for cell vitality by means of fluorescein diacetate, instead of SDH. The test materials included zinc phosphate cements, conventional and light-cured glass ionomer cements, a composite resin, and methylmethacrylate monomer. Four series of experiments were performed using L-929 mouse fibroblasts: (1) original method, (2) 24 h incubation time with procedural modifications; (3) use of FDA as marker for cell vitality; and (4) the agar overlay method. The staining intensity of the cells in the first series was insufficient. In the second series filter staining was good and showed distinct zones of damaged cells. In the third series cell staining was very distinct and easier to handle than with SDH. The results obtained in the second, third, and fourth series were in agreement with results from other cell culture tests. To compare results from different series an evaluation system based on the area of damaged cells was introduced. Correlations were high between series 2 and 3 as well as between 3 and 4. Our results indicate that the modifications of the filter test improve the method.

show abstract

“…The L-929 cells were used in agar diffusion and filter diffusion tests, which served as the screening tests. Originally, these tests were developed for testing the biocompatibility of materials and compounds coming into contact with the human body (11)(12)(13), and they are now accepted as an in vitro alternative to experiments conducted on laboratory animals (14). Hep 2 cells, used in the second part of our study, were chosen because of the known effect of chromium on the human respiratory system (15), also taking into consideration the previous studies on chromium toxicity employing the same model cells (3,16).…”

Section: Discussionmentioning

confidence: 99%

Toxic Effects of Chromium Acetate Hydroxide on Cells Cultivated In Vitro

2001

View full text Add to dashboard Cite

Many human activities, particularly industrial ones, result in an ever-growing production of toxic waste materials. The dynamics of the toxic effects of chromium acetate hydroxide, which is found in high concentrations in a waste sediment produced in the Czech Republic, were assessed by using a battery of in vitro tests carried out on two cell lines: L-929 (mouse fibroblasts) and Hep 2 (human laryngeal cells). Various markers of cell damage were assessed by phase-contrast, video and fluorescence microscopy, fluorometry, and DNA analysis. Chromium acetate hydroxide, over a concentration range of 1–0.02mol/l induced immediate cell death by fixation, whereas, at 0.002mol/l, the treated cells died in a much slower, more discrete manner. All the detected markers of cell damage, whether immediate or slow, clearly demonstrated that the cells died by necrosis. On the other hand, test concentration of 0.001mol/l appeared to constitute a threshold at which no pathological changes of Hep 2 cells were observed over 96 hours. We conclude that chromium acetate hydroxide has a high toxic potential in vitro, which should be considered when studying the toxicity of waste materials containing it.

show abstract

A modification of the cell culture agar diffusion test using fluoresceindiacetate staining

Cited by 23 publications

References 10 publications

Biocompatibility of dental alloys used in dental fixed prosthodontics

Biocompatibility of dental alloys used in dental fixed prosthodontics

New developments in the filter test system for cytotoxicity testing

Toxic Effects of Chromium Acetate Hydroxide on Cells Cultivated In Vitro

Contact Info

Product

Resources

About