A model of peroxidase activity with inhibition by hydrogen peroxide

Nicell, Jim A.; Wright, H. E.

doi:10.1016/s0141-0229(97)00001-x

Cited by 238 publications

(170 citation statements)

References 12 publications

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“…Since no significant increase in oxidation was observed with the 1:10 ratio, compared with the lower ones, this could mark the limit for the enzymatic oxidation. This is clear for HRP mediated reactions since a H 2 O 2 concentration higher than 5 mmol·L -1 might affect the enzyme, as shown by previous studies (Nicell and Wright, 1997;La Rotta et al 2007). However, these oxidation levels combined the enzymatic oxidation and the substrate CO due to H 2 O 2 , as previously shown.…”

Section: Enzymatic Oxidation Of (R)-limonene Employing H 2 O 2 Damentioning

confidence: 57%

“…On the other hand, the use of peroxidases in biocatalysis was also limited by the use of hydrogen peroxide as co-substrate, due to its inhibitory effect at high concentrations and slow reaction rates at lower concentrations (Nicell and Wright, 1997). One solution for this problem is the use of very low and controlled hydrogen peroxide supplementation, necessary just for the maintenance of an effective oxidation level, with no inhibitory effects.…”

mentioning

confidence: 99%

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Electrogeneration of hydrogen peroxide applied to the peroxide-mediated oxidation of (R)-limonene in organic media

Hernández¹,

Werberich²,

Mattos³

et al. 2007

Electron. J. Biotechnol.

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Section: Enzymatic Oxidation Of (R)-limonene Employing H 2 O 2 Damentioning

confidence: 57%

mentioning

confidence: 99%

Electrogeneration of hydrogen peroxide applied to the peroxide-mediated oxidation of (R)-limonene in organic media

Hernández¹,

Werberich²,

Mattos³

et al. 2007

Electron. J. Biotechnol.

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“…The AAP radicals then react with CTA radicals in solution and H 2 O 2 to produce blue quinoneimine dye. By increasing the AAP in the reagent mixture, the probability of direct oxidation of AAP by oxidised HRP is increased, which then increases the concentration of aminyl radicals and subsequently the probability of their dimerization [21]. The result of this is a decreased concentration of blue quinoneimine dye and an increased concentration of AAP dimers.…”

Section: Glucose Assay Optimisationmentioning

confidence: 99%

Fabrication and optimisation of a fused filament 3D-printed microfluidic platform

Tothill

Partridge

James

et al. 2017

J. Micromech. Microeng.

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A 3D-printed microfluidic device was designed and manufactured using a low cost ($2000) consumer grade fusion deposition modelling (FDM) 3D printer. FDM printers are not typically used, or are capable, of producing the fine detailed structures required for microfluidic fabrication. However, in this work, the optical transparency of the device was improved through manufacture optimisation to such a point that optical colorimetric assays can be performed in a 50 µl device. A colorimetric enzymatic cascade assay was optimised using glucose oxidase and horseradish peroxidase for the oxidative coupling of aminoantipyrine and chromotropic acid to produce a blue quinoneimine dye with a broad absorbance peaking at 590 nm for the quantification of glucose in solution. For comparison the assay was run in standard 96 well plates with a commercial plate reader. The results show the accurate and reproducible quantification of 0–10 mM glucose solution using a 3D-printed microfluidic optical device with performance comparable to that of a plate reader assay.

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“…Nicell and Wright [40] reported the dependence of horseradish peroxidase activity over a wide range of hydrogen peroxide concentrations. They observed an increase in the inhibitory effect on the enzyme catalytic activity.…”

Section: Electrochemical Behaviour Of the Biosensormentioning

confidence: 99%