A miRNA stabilizing polydopamine nano-platform for intraocular delivery of miR-21-5p in glaucoma therapy

Tan, Chen; Jia, Fan; Zhang, Peng; Sun, Xinghuai; Qiao, Yunsheng; Chen, Xueli; Wang, Youxiang; Chen, Junyi; Lei, Yuan

doi:10.1039/d0tb02881a

Cited by 22 publications

(21 citation statements)

References 40 publications

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“…The FT-IR spectra of PEI and PDA–PEI nanodots showed two weak peaks at 2942 and 2819 cm –1 , which were attributed to the C–H stretching vibration of the −CH 2 – spacer in PEI (Figure B) . A new absorption band at 1650 cm –1 was observed in the PDA–PEI nanodots, which was ascribed to the CN stretching vibration in the nanodot skeleton, which stemmed from the Michael addition reactions between the −NH 2 or PEI and the double bonds in DA. , …”

Section: Resultsmentioning

confidence: 96%

PDA–PEI-Copolymerized Nanodots with Tailorable Fluorescence Emission and Quenching Properties for the Sensitive Ratiometric Fluorescence Sensing of miRNA in Serum

Deng

Zang

et al. 2022

Anal. Chem.

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Dopamine and polyethyleneimine (PEI) copolymerized nanodots (PDA–PEI nanodots) with both fluorescence emission and quenching features were synthesized by a simple one-step reaction at room temperature. By adjusting the dopamine and PEI ratio as well as the chain length of PEI, the fluorescence emission and quenching properties of PDA–PEI nanodots can be controlled well. Under optimal conditions, the nanodots showed strong green fluorescence emission with an absolute quantum yield of 1–2% and a quenching efficiency of more than 99% to several fluorophores with emission wavelengths ranging from blue to red light regions. The nanodots with a large number of functional groups also showed strong affinity to nucleic acid strands, excellent solubility in aqueous solution, long-term stability, and uniform size distribution. Integrating these attractive features with the specific enzymatic digestion reaction of the DSN enzyme, a highly sensitive ratiometric fluorescence nanoprobe for miRNA analysis was developed. Aminomethylcoumarin acetate (AMCA), which possesses the same excitation wavelength but a well-resolved blue fluorescence emission with PDA–PEI nanodots, was selected as the signal-reporting unit for capture probe labeling, while the inherent green fluorescence of PDA–PEI nanodots served as the reference. According to the ratiometric fluorescence signal, the ratiometric fluorescence nanoprobes showed high sensitivity and good accuracy for the miRNA assay. Because of the high and universal quenching efficiency, stable fluorescence emission, easily assembled interface, and uniform morphology, the nanodots may have great application prospects to serve as a universal nanoplatform for the fabrication of ratiometric fluorescence nanoprobes.

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Section: Resultsmentioning

confidence: 96%

PDA–PEI-Copolymerized Nanodots with Tailorable Fluorescence Emission and Quenching Properties for the Sensitive Ratiometric Fluorescence Sensing of miRNA in Serum

Deng

Zang

et al. 2022

Anal. Chem.

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“…Finally, through intracameral injection, Tan et al (2021) delivered miRNA through polydopamine-polyethylenimine (PDA/PEI) Nanoparticles. The drug-delivery system improved penetration and stability, allowing for comparable transfection efficacy with lower cytotoxicity, and was also effective in lowering IOP in vivo [ 52 ].…”

Section: Nano-based Dds For Glaucomamentioning

confidence: 99%

Breaking Barriers in Eye Treatment: Polymeric Nano-Based Drug-Delivery System for Anterior Segment Diseases and Glaucoma

Ashkar

Jain

et al. 2023

Polymers

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The eye has anatomical structures that function as robust static and dynamic barriers, limiting the penetration, residence time, and bioavailability of medications administered topically. The development of polymeric nano-based drug-delivery systems (DDS) could be the solution to these challenges: it can pass through ocular barriers, offering higher bioavailability of administered drugs to targeted tissues that are otherwise inaccessible; it can stay in ocular tissues for longer periods of time, requiring fewer drug administrations; and it can be made up of polymers that are biodegradable and nano-sized, minimizing the undesirable effects of the administered molecules. Therefore, therapeutic innovations in polymeric nano-based DDS have been widely explored for ophthalmic drug-delivery applications. In this review, we will give a comprehensive overview of polymeric nano-based drug-delivery systems (DDS) used in the treatment of ocular diseases. We will then examine the current therapeutic challenges of various ocular diseases and analyze how different types of biopolymers can potentially enhance our therapeutic options. A literature review of the preclinical and clinical studies published between 2017 and 2022 was conducted. Thanks to the advances in polymer science, the ocular DDS has rapidly evolved, showing great promise to help clinicians better manage patients.

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“…Glaucoma is known to be the second leading cause of blindness worldwide [117,118]. Cellular senescence, mitochondrial dysfunction, oxidative stress, and neuroinflammation are all involved in the process of glaucoma.…”

Section: Glaucomamentioning

confidence: 99%

Immune modulating nanoparticles for the treatment of ocular diseases

Fang

Liu

et al. 2022

J Nanobiotechnol

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Ocular diseases are increasingly influencing people’s quality of life. Complicated inflammatory mechanisms involved in the pathogenic process of ocular diseases make inflammation-targeting treatment a potential therapeutic approach. The limited efficacy of conventional anti-inflammatory therapeutic strategies, caused by various objective factors, such as complex ocular biological barriers, and subjective factors, such as poor compliance, are promoting the development of new therapeutic methods. With the advantages of considerable tissue permeability, a controllable drug release rate, and selective tissue targeting ability, nanoparticles have successfully captured researchers’ attention and have become a research hotspot in treating ocular diseases. This review will focus on the advantages of nanosystems over traditional therapy, the anti-inflammation mechanisms of nanoparticles, and the anti-inflammatory applications of nanoparticles in different ocular diseases (ocular surface diseases, vitreoretinopathy, uveal diseases, glaucoma, and visual pathway diseases). Furthermore, by analyzing the current situation of nanotherapy and the challenges encountered, we hope to inspire new ideas and incentives for designing nanoparticles more consistent with human physiological characteristics to make progress based on conventional treatments. Overall, some progress has been made in nanoparticles for the treatment of ocular diseases, and nanoparticles have rather broad future clinical translation prospects.

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A miRNA stabilizing polydopamine nano-platform for intraocular delivery of miR-21-5p in glaucoma therapy

Abstract: Polydopamine–polyethylenimine nanoparticles (PDA/PEI NPs) have been successfully applied as miRNA carriers, and PDA/PEI NPs/miR-21-5p plays a role in increasing the aqueous humor drainage and lowering the intraocular pressure.

Cited by 22 publications

References 40 publications

PDA–PEI-Copolymerized Nanodots with Tailorable Fluorescence Emission and Quenching Properties for the Sensitive Ratiometric Fluorescence Sensing of miRNA in Serum

PDA–PEI-Copolymerized Nanodots with Tailorable Fluorescence Emission and Quenching Properties for the Sensitive Ratiometric Fluorescence Sensing of miRNA in Serum

Breaking Barriers in Eye Treatment: Polymeric Nano-Based Drug-Delivery System for Anterior Segment Diseases and Glaucoma

Immune modulating nanoparticles for the treatment of ocular diseases

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