A miniature microelectrode array to monitor the bioelectric activity of cultured cells

Thomas, Clémence; Springer, P.A.; Loeb, Gerald E.; Berwald‐Netter, Yoheved; Okun, Lawrence M.

doi:10.1016/0014-4827(72)90481-8

Cited by 514 publications

(309 citation statements)

References 4 publications

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“…Similar to previous reports (Thomas et al, 1972), we find that the effective electrode capacitance decreases by 50% or more after incubation with tissue culture medium. After 2 weeks in culture, electrode capacitances average 40009 1000 pF (averaged over 75 electrodes on five neurochips), still large enough for safe stimulation (see Section 3.3).…”

Section: Electrode Platinizationsupporting

confidence: 92%

“…Recording systems using planar arrays of extracellular metal electrodes (Thomas et al, 1972;Gross et al, 1977;Pine, 1980;Novak and Wheeler, 1988;Jimbo et al, 1993) or field-effect transistor electrodes (Stett et al, 1997) have been built to circumvent these problems. Electrodes are arranged on the surface of a glass or silicon substrate, upon which neurons are cultured.…”

Section: Introductionmentioning

confidence: 99%

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The neurochip: a new multielectrode device for stimulating and recording from cultured neurons

Maher

Pine

Wright

et al. 1999

Journal of Neuroscience Methods

304

180

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Section: Electrode Platinizationsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

The neurochip: a new multielectrode device for stimulating and recording from cultured neurons

Maher

Pine

Wright

et al. 1999

Journal of Neuroscience Methods

304

180

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“…Taking advantage of MEA-based recording for neuronal network activity, we also measured the electrical activity of cultured cortical networks (Thomas et al, 1972;Gross et al, 1977;Pine, 1980). The networks showed synchronized bursts, and the network activity (firing rate and synchronized burst rate) became saturated, with large deviations, after initially increasing during the 1-month culture period under this culture condition (Fig.…”

Section: Relationship Between Synaptic Densities and Network Electricmentioning

confidence: 99%

Measurement of saturation processes in glutamatergic and GABAergic synapse densities during long-term development of cultured rat cortical networks

2013

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The aim of this study was to clarify the saturation processes of excitatory and inhibitory synapse densities during the long-term development of cultured neuronal networks. For this purpose, we performed a long-term culture of rat cortical cells for 35 days in vitro (DIV). During this culture period, we labeled glutamatergic and GABAergic synapses separately using antibodies against vesicular glutamate transporter 1 (VGluT1) and vesicular transporter of -aminobutyric acid (VGAT). The densities and distributions of both types of synaptic terminals were measured simultaneously. Observations and subsequent measurements of immunofluorescence demonstrated that the densities of both types of antibody-labeled terminals increased gradually from 7 to 21-28 DIV. The densities did not show a further increase at 35 DIV and tended to become saturated.Triple staining with VGluT1, VGAT, and microtubule-associated protein 2 (MAP2) enabled analysis of the distribution of both types of synapses, and revealed that the densities of the two types of synaptic terminals on somata were not significantly different, but that glutamatergic synapses predominated on the dendrites during long-term culture.However, some neurons did not fall within this distribution, suggesting differences in synapse distribution on target neurons. The electrical activity also showed an initial increase and subsequent saturation of the firing rate and synchronized burst rate during long-term culture, and the number of days of culture to saturation from the initial increase followed the same pattern under this culture condition.

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“…161 Multiple electrode arrays (MEAs) of up to 100 electrodes patterned by photolithography on glass or silicon substrates have been used for decades; transparent MEAs can be made of indium tin oxide. 162,163 Planar MEAs, consisting of 10-100 electrode sites (with diameters on the order of the cells-i.e., ranging from a few microns up to a few tens of microns) spaced at 100-200 μm interdistance on glass plates, were used by Tomas et al, 164 Gross et al, 165 Novak and Wheeler, 166 and many others followed in studies of the activity and plasticity of developing cultured neuronal networks 167,168 or slices. 162,163,[169][170][171][172][173] MEAs are now commercially available in a variety of formats.…”

Section: Extracellular Electrode Arrays For Multisite Recording and Smentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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The ability to culture cells in vitro has revolutionized hypothesis testing in basic cell and molecular biology research and has become a standard methodology in drug screening and toxicology assays. However, the traditional cell culture methodology-consisting essentially of the immersion of a large population of cells in a homogeneous fluid medium-has become increasingly limiting, both from a fundamental point of view (cells in vivo are surrounded by complex spatiotemporal microenvironments) and from a practical perspective (scaling up the number of fluid handling steps and cell manipulations for high-throughput studies in vitro is prohibitively expensive). Micro fabrication technologies have enabled researchers to design, with micrometer control, the biochemical composition and topology of the substrate, the medium composition, as well as the type of neighboring cells surrounding the microenvironment of the cell. In addition, microtechnology is conceptually well suited for the development of fast, low-cost in vitro systems that allow for high-throughput culturing and analysis of cells under large numbers of conditions. Here we review a variety of applications of microfabrication in cell culture studies, with an emphasis on the biology of various cell types.

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A miniature microelectrode array to monitor the bioelectric activity of cultured cells

Cited by 514 publications

References 4 publications

The neurochip: a new multielectrode device for stimulating and recording from cultured neurons

The neurochip: a new multielectrode device for stimulating and recording from cultured neurons

Measurement of saturation processes in glutamatergic and GABAergic synapse densities during long-term development of cultured rat cortical networks

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

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