A mineralocorticoid-like receptor in the rainbow trout, Oncorhynchus mykiss: cloning and characterization of its steroid binding domain

Colombe, Laurent; Fostier, Alexis; Bury, Nicolas R.; Pakdel, Farzad; Guiguen, Yann

doi:10.1016/s0039-128x(00)00090-8

Cited by 132 publications

(74 citation statements)

References 37 publications

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“…1. The salmon sequence is 98.5% identical to the published rainbow trout GR sequence (Ducouret et al, 1995) and 39.7% identical to the rainbow trout mineralocorticoid-like receptor sequence (Colombe et al, 2000) at amino acid levels. Fig.…”

Section: Partial Masu Salmon Cr Cdna Cloning and Sequence Determinationmentioning

confidence: 74%

“…CR mRNA and protein have been localized to the gill chloride cell in chum salmon (Uchida et al, 1998). Besides, mineralocorticoid-like receptor, which is closely related to GR, cDNA has been recently isolated from rainbow trout testis (Colombe et al, 2000). Nonetheless, changes in CR transcript levels during smoltification have not been studied.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Changes in Transcript Levels of Gill Cortisol Receptor during Smoltification in Wild Masu Salmon, Oncorhynchus masou

Mizuno

Ura²,

Onodera³

et al. 2001

Zoological Science

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Section: Partial Masu Salmon Cr Cdna Cloning and Sequence Determinationmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Changes in Transcript Levels of Gill Cortisol Receptor during Smoltification in Wild Masu Salmon, Oncorhynchus masou

Mizuno

Ura²,

Onodera³

et al. 2001

Zoological Science

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“…(Orthologs are related genes in different genomes, descended from a speciation event; paralogs are related genes in the same genome, descended from a gene duplication.) Orthologs of all steroid receptors present in tetrapods have been identified in teleosts (7,8), indicating that all six types existed by the time ray-finned fish split from the lineage leading to tetrapods some 400 million years ago (9). PCR screens have identified short fragments of an ER, GR, and AR in shark and a single steroid receptor of indeterminate type in hagfish (8), but this approach cannot distinguish a failure to amplify a gene from its true absence in an organism.…”

mentioning

confidence: 99%

Evolution of vertebrate steroid receptors from an ancestral estrogen receptor by ligand exploitation and serial genome expansions

Thornton

2001

Proc. Natl. Acad. Sci. U.S.A.

629

472

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The evolution of novelty in tightly integrated biological systems, such as hormones and their receptors, seems to challenge the theory of natural selection: it has not been clear how a new function for any one part (such as a ligand) can be selected for unless the other members of the system (e.g., a receptor) are already present. Here I show-based on identification and phylogenetic analysis of steroid receptors in basal vertebrates and reconstruction of the sequences and functional attributes of ancestral proteins-that the first steroid receptor was an estrogen receptor, followed by a progesterone receptor. Genome mapping and phylogenetic analyses indicate that the full complement of mammalian steroid receptors evolved from these ancient receptors by two large-scale genome expansions, one before the advent of jawed vertebrates and one after. Specific regulation of physiological processes by androgens and corticoids are relatively recent innovations that emerged after these duplications. These findings support a model of ligand exploitation in which the terminal ligand in a biosynthetic pathway is the first for which a receptor evolves; selection for this hormone also selects for the synthesis of intermediates despite the absence of receptors, and duplicated receptors then evolve affinity for these substances. In this way, novel hormone-receptor pairs are created, and an integrated system of increasing complexity elaborated. This model suggests that ligands for some ''orphan'' receptors may be found among intermediates in the synthesis of ligands for phylogenetically related receptors.

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“…In fish it has been shown that cortisol transactivate in vitro the two glucocorticoid receptors (Bury et al, 2003). Interestingly, a mineralocorticoid-like receptor initially isolated from a testes cDNA library was described to have high affinity for cortisol (Colombe et al, 2000). The receptivity for the cortisol is present in fish testes but receptors by which they exert a physiological effect are not well understood.…”

Section: Discussionmentioning

confidence: 99%

17,20β-P and cortisol are the main in vitro metabolites of 17-hydroxy-progesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae)

Cantonnet¹,

Mateo²,

Alberro³

et al. 2015

Neotrop. ichthyol.

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The aim was to investigate the major C21 steroids produced by spermiating white croaker Micropogonias furnieri (Sciaenidae) in order to establish the potential mediator of gamete maturation in males of this species. The testes steroid production at the spawning season was identified incubating the 3 H-17-hydroxy-4-pregnene-3,20-dione precursor through thin layer chromatography, high pressure liquid chromatography, enzymatic oxydation, acetylation and immunochemistry analyses. 17,17, were the main metabolites produced. Contrary to what we expected, 17,20β,21-Trihydroxy-4-pregnen-3-one was not detected. Circulating levels of 17,20β-P were undetectable in immature testes and in those at the first spermatogenesis stages, while a clear increase was observed during the whole spermatogenesis and spermiation phases (from undetectable to 1047 pg mL -1 ).In vitro studies together with plasma detection suggest that 17,20β-P is a good steroid candidate involved in M. furnieri testes maturation. The role of cortisol during late phases of testes development needs further studies.El objetivo fue investigar cuales eran los esteroides C21 más importantes producidos por los testículos en espermiación de la corvina blanca Micropogonias furnieri (Sciaenidae) para poder identificar los potenciales mediadores de la maduración gamética de los machos de esta especie. Los esteroides producidos por los testículos en espermiación fueron identificados después de su incubación con el precursor 3 H-17-hidroxi-4-pregnene-3,20-diona a través de cromatografía de capa fina y cromatografía líquida de alta presión y posteriormente por oxidación enzimática, acetilación e inmunoquímica. Los principales metabolitos producidos por los testículos en espermiación fueron la 17,20β-Dihidroxi-4-pregnen-3-ona (17,20β-P) y la 11β,17,21-Trihidroxi-4-pregnene-3,20-diona (cortisol). Contrariamente a lo esperado, no se encontró el derivado tri-hidroxilado de la progesterona llamado 17,20β,21-Trihidroxi-4-pregnen-3-ona. Los niveles circulantes de 17,20β-P fueron no detectable en los testículos inmaduros y en aquellos en inicios de espermatogénesis, mientras que un aumento claro en las concentraciones circulantes fue detectada en corvinas en plena espermatogénesis y en espermiación (desde no detectable a 1047 pg mL -1 ). Los resultados obtenidos in vitro junto a los cambios a nivel plasmático sugieren que la 17,20β-P es un buen candidato a proponer como esteroide involucrado en la regulación del proceso de maduración testicular de la corvina. La función del cortisol a nivel testicular debería ser mejor estudiada en las etapas finales del desarrollo testicular de esta especie.

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A mineralocorticoid-like receptor in the rainbow trout, Oncorhynchus mykiss: cloning and characterization of its steroid binding domain

Cited by 132 publications

References 37 publications

Changes in Transcript Levels of Gill Cortisol Receptor during Smoltification in Wild Masu Salmon, Oncorhynchus masou

Changes in Transcript Levels of Gill Cortisol Receptor during Smoltification in Wild Masu Salmon, Oncorhynchus masou

Evolution of vertebrate steroid receptors from an ancestral estrogen receptor by ligand exploitation and serial genome expansions

17,20β-P and cortisol are the main in vitro metabolites of 17-hydroxy-progesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae)

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