A Micropropagation Protocol for Mass Multiplication and Off-Site Conservation ofCelastrus paniculatus-

In vitro propagation, micromorphological studies and ex vitro rooting of cannon ball tree (Couroupita guianensis aubl.): a multipurpose threatened species

“…The effectiveness of BAP over Kin in bud breaking and shoot proliferation has been well documented in several plant species (Singh and Tiwari 2012;Shekhawat et al 2015b;Patel et al 2014). The rejuvenation of meristem was achieved in plants by selecting the age of the mother plant, nature of explant, season of explant collection, plant growth regulators and additives (ascorbic and citric acids, adenine sulphate and arginine) used (Arya et al 2002).…”

Development of a Successful Protocol for <i>in vitro</i> Mass Propagation of <i>Celastrus paniculatus</i> Willd. – A Valuable Medicinal Plant

“…Organogenesis depends upon hormonal balance and other factors such as plant tissue, environmental conditions, composition of the medium, polarity and growth substances (Razdan, 1994). Shoots were induced in MS medium supplemented with 8.44 µM BAP in C. paniculatus (Rao and Purohit, 2006) while in another study MS medium supplemented with 1.5 mg/L BAP, 0.1 mg/L NAA, 50.0 mg/L ascorbic acid, 25.0 mg/L each of adenine sulfate, arginine and citric acid as additives (Arya et al, 2001). Although MS medium as well as WPM were used as basal media, it was observed that MS provides better medium for C. paniculatus.…”

“…However, a mixture of top soil: coir dust: compost also gave a significantly higher survival percentage (75%) suggesting both mixtures could be used as substrates for accilimatization of tissue cultured C. paniculatus plants. Use of a mixture of black soil: fine sand: farmyard manure: soilrite (4:2:1:1) gave only 53% survival (Arya et al, 2001).…”

“…In Sri Lanka, this species is very rare and only few live specimens are to be found. This species has been identified as a threatened plant, requiring scientific efforts for conservation and commercial cultivation (Arya et al, 2001).…”

In vitro Effect of Various Plant Growth Regulator on Micro Propagation of Celastrus paniculatus: An Important Medicinal Plant

“…They induced Multiple shoots in the MS media with 5.0 μM BAP and 0.5 μM IAA. Arya et al (2002) reported multiplication in a medium containing NAA and BA. Martin et al, (2006) achieved Maximum shoot induction (five shoots per explant) in MS medium supplemented with 1.5 mg l−1 BA and 0.1 mg l−1 NAA.…”