A Microfluidic System for One‐Chip Harvesting of Single‐Cell‐Laden Hydrogels in Culture Medium

Nan, Lang; Yang, Zhenyu; Lyu, Hao; Lau, Kitty Yu Yeung; Shum, Ho Cheung

doi:10.1002/adbi.201900076

Cited by 21 publications

(21 citation statements)

References 39 publications

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“…Therefore, the type of crosslinking dictates droplet formation and adjustments that need to be made to device design. Commonly used types are ionic cross-linking (Choi et al, 2007;Workman et al, 2007;Utech et al, 2015;Ahmed and Stokke, 2021), photo-cross-linking (Zhao et al, 2016;Mohamed et al, 2019;Nan et al, 2019), and thermoresponsive cross-linking (Dolega et al, 2015;Yanakieva et al, 2020;Tiemeijer et al, 2021;Zhang et al, 2021). By design, the latter two do not require on-chip mixing of different aqueous phases and can still be used in conventional device designs.…”

Section: Microfluidicsmentioning

confidence: 99%

Hydrogels for Single-Cell Microgel Production: Recent Advances and Applications

Tiemeijer

Tel

2022

Front. Bioeng. Biotechnol.

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Single-cell techniques have become more and more incorporated in cell biological research over the past decades. Various approaches have been proposed to isolate, culture, sort, and analyze individual cells to understand cellular heterogeneity, which is at the foundation of every systematic cellular response in the human body. Microfluidics is undoubtedly the most suitable method of manipulating cells, due to its small scale, high degree of control, and gentle nature toward vulnerable cells. More specifically, the technique of microfluidic droplet production has proven to provide reproducible single-cell encapsulation with high throughput. Various in-droplet applications have been explored, ranging from immunoassays, cytotoxicity assays, and single-cell sequencing. All rely on the theoretically unlimited throughput that can be achieved and the monodispersity of each individual droplet. To make these platforms more suitable for adherent cells or to maintain spatial control after de-emulsification, hydrogels can be included during droplet production to obtain “microgels.” Over the past years, a multitude of research has focused on the possibilities these can provide. Also, as the technique matures, it is becoming clear that it will result in advantages over conventional droplet approaches. In this review, we provide a comprehensive overview on how various types of hydrogels can be incorporated into different droplet-based approaches and provide novel and more robust analytic and screening applications. We will further focus on a wide range of recently published applications for microgels and how these can be applied in cell biological research at the single- to multicell scale.

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Section: Microfluidicsmentioning

confidence: 99%

Hydrogels for Single-Cell Microgel Production: Recent Advances and Applications

Tiemeijer

Tel

2022

Front. Bioeng. Biotechnol.

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“…In recent years, with the breakthrough progress of materials science, micro-nanoprocessing technology and microelectronics, microfluidic chips have been developed rapidly. [121,122] Specially, utilizing the structural characteristics of microchannels and microwells in the microfluidic chips, cell suspension can be injected into the chips and form spheroids. [96,97] Hierlemann's group described a hanging drop microfluidic device composed of microchannels and microwells, with PDMS as the main material.…”

Section: Spheroids Formation In the Microfluidic Channelsmentioning

confidence: 99%

Development of Cell Spheroids by Advanced Technologies

Shao

Chi

Zhang

et al. 2020

Adv Materials Technologies

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3D cell culture has been strongly advocated as a highly useful culture technique instead of the traditional 2D cell culture. Specially, spheroids, as the primary mode of 3D cell culture, allow cells to establish a connection between the cell and the extracellular matrix to form a specific 3D structure that better mimics the growing environment of cells in vivo. Benefiting from the emergence of various advanced micromachining platforms, spheroids have received increasing attention in diverse fields. Herein, the combination of the cell spheroid culture with microfluidic technology is reviewed. After concisely introducing the traditional methods for fabricating cell spheroids, an outline of the approaches for preparing cell spheroids using different advanced techniques is given. Then, the various applications of the generated cell spheroids in the field of biomedical engineering are presented and the current limitations, challenges, and future directions are summarized.

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“…In addition, other microfluidic approaches based on GelMA hydrogels were studied in biomedical fields recently [ 74 , 83 , 99 ].…”

Section: Applications Of Gelma Hydrogels As Simulation Units In Timentioning

confidence: 99%

“…Therefore, cell encapsulation in 3-D hydrogel posts within the microfluidic channels was used to enhance the capabilities of the microfluidic devices. Nan et al [ 99 ] developed a one-chip harvesting of single cell-laden GelMA hydrogels based on a microfluidic system in culture medium. The hydrogels were generated by using an on-chip gelation technique, which included droplet generation under UV light.…”

Section: Applications Of Gelma Hydrogels As Simulation Units In Timentioning

confidence: 99%

Applications of Gelatin Methacryloyl (GelMA) Hydrogels in Microfluidic Technique-Assisted Tissue Engineering

et al. 2020

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In recent years, the microfluidic technique has been widely used in the field of tissue engineering. Possessing the advantages of large-scale integration and flexible manipulation, microfluidic devices may serve as the production line of building blocks and the microenvironment simulator in tissue engineering. Additionally, in microfluidic technique-assisted tissue engineering, various biomaterials are desired to fabricate the tissue mimicking or repairing structures (i.e., particles, fibers, and scaffolds). Among the materials, gelatin methacrylate (GelMA)-based hydrogels have shown great potential due to their biocompatibility and mechanical tenability. In this work, applications of GelMA hydrogels in microfluidic technique-assisted tissue engineering are reviewed mainly from two viewpoints: Serving as raw materials for microfluidic fabrication of building blocks in tissue engineering and the simulation units in microfluidic chip-based microenvironment-mimicking devices. In addition, challenges and outlooks of the exploration of GelMA hydrogels in tissue engineering applications are proposed.

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A Microfluidic System for One‐Chip Harvesting of Single‐Cell‐Laden Hydrogels in Culture Medium

Cited by 21 publications

References 39 publications

Hydrogels for Single-Cell Microgel Production: Recent Advances and Applications

Hydrogels for Single-Cell Microgel Production: Recent Advances and Applications

Development of Cell Spheroids by Advanced Technologies

Applications of Gelatin Methacryloyl (GelMA) Hydrogels in Microfluidic Technique-Assisted Tissue Engineering

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