Applications of Gelatin Methacryloyl (GelMA) Hydrogels in Microfluidic Technique-Assisted Tissue Engineering

Liu, Taotao; Weng, Wenxian; Zhang, Yuzhuo; Sun, Xiaoting; Yang, Haw

doi:10.3390/molecules25225305

Cited by 53 publications

(25 citation statements)

References 100 publications

(122 reference statements)

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“…Depending on the source, hydrogels can be divided into natural hydrogels and synthetic hydrogels. Natural hydrogels (e.g., HA, alginate, chitosan, collagen, and polyethylene glycol) have already been widely used in the field of regenerative medicine ( Liu et al, 2020a ). They are generally highly biocompatible, as reflected in their successful use in the peritoneum and other sites in vivo ( T et al, 2016 ).…”

Section: The Design Of Hydrogels For Iuamentioning

confidence: 99%

Application of Bioactive Hydrogels for Functional Treatment of Intrauterine Adhesion

Wang

Yang

Xie

et al. 2021

Front. Bioeng. Biotechnol.

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Intrauterine adhesion (IUA) is a common endometrial disease and one of the main causes of infertility in women of childbearing age. Current treatment strategies, such as hysteroscopic adhesion resection, hysteroscopic transcervical resection of adhesion (TCRA), the use of local hormone drugs, and anti-adhesion scaffold implantation, do not provide a satisfactory pregnancy outcome for moderate-severe IUA, which presents a great challenge in reproductive medicine. With the development of material engineering, various bioactive and functional hydrogels have been developed using natural and synthetic biomaterials. These hydrogels are not only used as barely physical barriers but are also designed as vectors of hormone drugs, growth factors, and stem cells. These characteristics give bioactive hydrogels potentially important roles in the prevention and treatment of IUA. However, there is still no systematic review or consensus on the current advances and future research direction in this field. Herein, we review recent advances in bioactive hydrogels as physical anti-adhesion barriers, in situ drug delivery systems, and 3D cell delivery and culture systems for seeded cells in IUA treatment. In addition, current limitations and future perspectives are presented for further research guidance, which may provide a comprehensive understanding of the application of bioactive hydrogels in intrauterine adhesion treatment.

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Section: The Design Of Hydrogels For Iuamentioning

confidence: 99%

Application of Bioactive Hydrogels for Functional Treatment of Intrauterine Adhesion

Wang

Yang

Xie

et al. 2021

Front. Bioeng. Biotechnol.

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“…In particular, the FRESH method allows integration of thermosensitive hydrogels such as gelatin and Pluronics for the generation of hollow structures [34]. Additionally, direct bioprinting of perfusable constructs is possible using multiple print-heads containing an outer cross-linkable hydrogel (e.g., GelMA) and an inner head with the appropriate cross-linking solution [35].…”

Section: A D V a N C E D O N L I N E A R T I C L Ementioning

confidence: 99%

Current state and future of 3D bioprinted models for cardio-vascular research and drug development

Polonchuk

Gentile

2021

ADMET DMPK

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In the last decade, 3D bioprinting technology has emerged as an innovative tissue engineering approach for regenerative medicine and drug development. This article aims at providing an overview about the most commonly used bioengineered tissues, focusing on 3D bioprinted cardiac cells and how they have been utilized for drug discovery and development. The review describes that, while this field is still developing, cardiovascular research may benefit from laboratory-engineered heart tissues built of specific cell types with precise 3D architecture mimicking the native cardiac microenvironment. It also describes the role played by regulatory agencies and potential commercialization pathways for direct translation from the bench to the bedside of studies using 3D bioprinted cardiac tissues. ©2021 by the authors. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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“…This study aims to develop a bioink that can satisfy both biocompatibility and printability all at once by employing the optimized composition of four different biomaterials, such as gelatin methacryloyl (GelMA), alginate, carboxymethyl cellulose (CMC), and xanthan gum (XG). GelMA is a hydrophilic light-responsive polymer produced by reacting methacrylic anhydride with gelatin and has an advantage of providing good cell adhesion along with tunable physicochemical properties [ 28 ]. In particular, GelMA-based bioink has been proven to show characteristics similar to natural ECM and has been widely used for 3D cell culture and tissue formation [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Polysaccharide Hydrocolloid for the Development of Bioink with High Printability/Biocompatibility for Coextrusion 3D Bioprinting

et al. 2021

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Bioink is the main component of 3D bioprinting process and is crucial for the generation of sophisticated 3D structures through precise spatial control. Therefore, bioink’s core material must have characteristics that support good printability as well as biocompatibility. However, there is a lack of bioinks developed that satisfy these characteristics at the same time. In this work, our aim was to develop a bioink that satisfies the needs for both printability and biocompatibility through effectively utilizing hydrocolloid materials. To do so, carboxymethyl cellulose (CMC) and xanthan gum (XG) were used to maintain proper shear properties at high pressure and increase the mechanical properties of bioink without excessively affecting the viscosity, and thus enhance printability and biocompatibility. Various bioink formulations were applied to 3D printing process and the printability optimization was carried out through adjusting the hydrocolloid contents in connection with different cross-linking methods. Through utilization of hydrocolloids, the printability and rheological analysis showed that the bioink has improved mechanical properties and confirmed that the printability could be adjusted by controlling the CMC and XG ratio. Moreover, cell viability and immunocytochemical staining analyses showed cell compatibility with enhanced stability. The proposed convenient method to control the printability with improved biocompatibility suggests more appropriate use of bioink for co-axial 3D bioprinting.

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Applications of Gelatin Methacryloyl (GelMA) Hydrogels in Microfluidic Technique-Assisted Tissue Engineering

Cited by 53 publications

References 100 publications

Application of Bioactive Hydrogels for Functional Treatment of Intrauterine Adhesion

Application of Bioactive Hydrogels for Functional Treatment of Intrauterine Adhesion

Current state and future of 3D bioprinted models for cardio-vascular research and drug development

Optimization of Polysaccharide Hydrocolloid for the Development of Bioink with High Printability/Biocompatibility for Coextrusion 3D Bioprinting

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